A high-sugar diet produces obesity and insulin resistance in wild-type <i>Drosophila</i>

Musselman, Laura Palanker; Fink, Jill L.; Narzinski, Kirk; Ramachandran, Prasanna Venkatesh; Hathiramani, Sumitha S.; Cagan, Ross; Baranski, Thomas J.

doi:10.1242/jcs.102947

Cited by 94 publications

(147 citation statements)

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“…This effect is likely advantageous to Drosophila feeding on many natural fruits (sugar content 2.5-16% (http://ndb.nal.usda.gov/ndb/ foods)) because Drosophila reared on sugar-rich diets display reduced feeding rates, resulting in inadequate acquisition of other limiting nutrients, as well as hyperlipidaemia, hyperglycaemia and insulin resistance [9,10]. Our results are also relevant to the use of Drosophila in nutritional research, especially as a biomedical model for metabolic diseases, including obesity [9]. These experiments should include empirical determination of the nutrient content of food, to account for likely microbial-mediated changes to diet composition.…”

Section: Discussionmentioning

confidence: 99%

Consumption of dietary sugar by gut bacteria determinesDrosophilalipid content

Huang

Douglas

2015

Biol. Lett.

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Gut microorganisms are essential for the nutritional health of many animals, but the underlying mechanisms are poorly understood. This study investigated how lipid accumulation by adult Drosophila melanogaster is reduced in flies associated with the bacterium Acetobacter tropicalis which displays oralfaecal cycling between the gut and food. We demonstrate that the lower lipid content of A. tropicalis-colonized flies relative to bacteria-free flies is linked with a parallel bacterial-mediated reduction in food glucose content; and can be accounted for quantitatively by the amount of glucose acquired by the flies, as determined from the feeding rate and assimilation efficiency of bacteria-free and A. tropicalis-colonized flies. We recommend that nutritional studies on Drosophila include empirical quantification of food nutrient content, to account for likely microbial-mediated effects on diet composition. More broadly, this study demonstrates that selective consumption of dietary constituents by microorganisms can alter the nutritional balance of food and, thereby, influence the nutritional status of the animal host.

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Section: Discussionmentioning

confidence: 99%

Consumption of dietary sugar by gut bacteria determinesDrosophilalipid content

Huang

Douglas

2015

Biol. Lett.

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“…Knockdown was confirmed by semiquantitative RT-PCR of RNA from transgene-expressing flies. Diets were a modification of Bloomington semi-defined food and contained 0.15 or 0.7 M sucrose as the sugar source as described previously (4). Larvae were reared from egg lay until wandering third instar and harvested from the vial wall for all experiments except tracer studies.…”

Section: Uas-dcr2 Uas-mio Imentioning

confidence: 99%

“…When fed excess calories in the form of an HSD, larvae routed energy from the diet into lipid storage in the fat body (4). We hypothesized that in the face of chronic exposure to HSD this storage capacity is limiting, leading to altered processing of dietary carbon and the model T2D phenotypes.…”

Section: Metabolic Channeling Of Glucose Is Reduced By Hsd Feeding-mentioning

confidence: 99%

“…Lean Larvae Are Intolerant of an HSD-In response to excessive nutrient consumption, both mammals and flies increase fatty acid synthesis and esterification into TAG storage pools (2,4,38,39). We previously observed that HSD-fed wandering third instar larvae exhibited gene expression changes that reflect increased lipogenesis and decreased lipolysis (4).…”

Section: The Fat Body Controls Metabolic Homeostasismentioning

confidence: 99%

“…More recently, caloric excess has been shown to elicit many of the same negative consequences in model organisms as have long been observed in humans (1)(2)(3)(4)(5)(6)(7). Work from our laboratory and that of others has shown that feeding Drosophila a high sugar diet (HSD) 2 results in a model of type 2 diabetes (T2D) that includes hyperglycemia, obesity, insulin resistance, cardiac arrhythmias, and reduced lifespan (4,8,73).…”

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Role of Fat Body Lipogenesis in Protection against the Effects of Caloric Overload in Drosophila

Musselman

Fink

Ramachandran

et al. 2013

Journal of Biological Chemistry

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Sargassum fusiforme polysaccharide attenuates high‐sugar–induced lipid accumulation in HepG2 cells and Drosophila melanogaster larvae

Yan

Zhang

et al. 2021

Food Science & Nutrition

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Chronic consumption of sugar-rich beverages or foods promotes the development of non-alcoholic fatty liver disease (NAFLD) and reduces life expectancy (Tilman & Clark, 2014). Among the various sugars, sucrose is widely used as an inexpensive sweetener in beverages or foods; upon ingestion, sucrose is converted to glucose and fructose, which are subsequently absorbed and transported by the blood to the cells and tissues to be further metabolized. In humans, excessive sugar intake disrupts normal lipid metabolism and

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A high-sugar diet produces obesity and insulin resistance in wild-type Drosophila

Cited by 94 publications

References 1 publication

Consumption of dietary sugar by gut bacteria determinesDrosophilalipid content

Consumption of dietary sugar by gut bacteria determinesDrosophilalipid content

Role of Fat Body Lipogenesis in Protection against the Effects of Caloric Overload in Drosophila

Sargassum fusiforme polysaccharide attenuates high‐sugar–induced lipid accumulation in HepG2 cells and Drosophila melanogaster larvae

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