Role of Fat Body Lipogenesis in Protection against the Effects of Caloric Overload in Drosophila

Drosophilaas a model to study obesity and metabolic disease

“…Lipogenesis is regulated by a number of factors and substrates other than IIS, including acetyl-CoA and the activated carrier NADPH (Teesalu et al, 2017). The conserved transcription factors sterol regulatory element-binding protein (SREBP) and carbohydrate-responsive element-binding protein (ChREBP; also called Mondo and Mio in the fly) promote the expression of lipogenic enzymes such as fatty acid synthase, stearoylCoA desaturase and diacylglycerol O-acyltransferase 1 (DGAT1; also called Midway in flies), which all increase triglyceride storage (Buszczak et al, 2002;Garrido et al, 2015;Havula et al, 2013;Kunte et al, 2006;Musselman et al, 2013;Sassu et al, 2012). Other genes required for lipogenesis in the fat body include those encoding the phosphatidate phosphatase dLipin (Schmitt et al, 2015;Ugrankar et al, 2011), pantothenate kinase and phosphopantothenoylcysteine synthase (Musselman et al, 2016) and Myc (Parisi et al, 2013).…”

CoA protects against the deleterious effects of caloric overload in Drosophila

“…TG, FFA, and glycogen were assayed from groups of six frozen wandering L3 larvae as previously described ( 17 ). Hemolymph was isolated from groups of fi ve to twenty wandering larvae and assayed as previously described ( 17 ).…”

“…Homogenates were defrosted, TCA precipitated, and washed six times with ether to remove lipids. Aqueous fractions were dried, resuspended, and run on a Waters HPLC, as described ( 17 ).…”

“…Hemolymph was isolated from groups of fi ve to twenty wandering larvae and assayed as previously described ( 17 ).…”

Influence of Dietary Yeast Restriction on Pathological Changes in the Body of the Fruit Fly under High Consumption of Amylose Starch

“…Однак експери-ментально доведено, що у лабораторних умовах цих комах можна сміливо використовувати як мо-дель для дослідження метаболічних перебудов при надмірному споживанні вуглеводів [3,5,14]. Існує фенотипова подібність метаболічних пору-шень, пов'язаних з ожирінням, у безхребетних та хребетних тварин, індукована переїданням моно-та дисахаридів [13][14][15]. Однак даних щодо відмін-ностей між моно-та полісахаридами на метаболіч-ні показники дрозофіл досі недостатньо.…”