A high sensitivity wireless mass-loading surface acoustic wave DNA biosensor

Abstract

In this paper, a surface acoustic wave (SAW) biosensor with gold delay area on LiNbO 3 substrate detecting DNA sequences is proposed. By well-designed device parameters of the SAW sensor, it achieves a high performance for highly sensitive detection of target DNA. In addition, an effective biological treatment method for DNA immobilization and abundant experimental verification of the sensing effect have made it a reliable device in DNA detection. The loading mass of the probe and target DNA sequences is obtained from the frequency shifts, which are big enough in this work due to an effective biological treatment. The experimental results show that the biosensor has a high sensitivity of 1.2 pg/ml/Hz and high selectivity characteristic is also verified by the few responses of other substances. In combination with wireless transceiver, we develop a wireless receiving and processing system that can directly display the detection results.

Emerging on-chip surface acoustic wave technology for small biomaterials manipulation and characterization

“…Recently, several works show that the concentration of ssDNAs can actually be quantified, where the concentration is in proportional to the measured phase shift of the SAW. 55,[60][61][62][63] Some of these SAW sensors can detect the mismatch with the resolution of up to single base pair. Such high sensitivity can be achieved by applying GHz SAW as reported by Cai et al, 60 employing silver-nanoparticle amplification technique as reported by Zhang et al 62 and coating the piezoelectric surface with a sensitive layer as reported by Ji et al 61 Sensing the mismatch is important in modern structural DNA nanotechnology where ssDNAs are used to self-assemble complicated structures for different applications, 64 as the mismatch is an indicator of the defect in the DNA-assembled biomaterials.…”

Development and Applications of Portable Biosensors

“…212 An implantable wireless biosensor for the immediate detection of upper gastrointestinal bleeding 213 has been developed. A high-sensitivity wireless mass-loading SAW-based DNA biosensor 214 has been developed. Continuous monitoring and evaluation of the prognostics of human health require biosensor networks to be deployed both in the environment and on patients.…”

Wireless Biological Electronic Sensors

“…Figure 2 a shows a two-port SAW-based wireless biosensor, using a delay line to transmit the signal [ 64 ]. The gold interdigital electrodes and the center gold delay area are fabricated onto a piezoelectric substrate by microfabrication.…”

“…( a ) Two-port SAW-based biosensor with antenna. Reprinted with permission from [ 64 ]. Copyright (2014) Worldscientific.…”