A high-resolution radiation hybrid map of the river buffalo major histocompatibility complex and comparison with BoLA

Stafuzza, Nedenia Bonvino; Greco, Anthony J.; Grant, Jason R.; Abbey, Colette A.; Gill, C. A.; Raudsepp, Terje; Skow, Loren C.; Womack, James E.; Riggs, Penny K.; Amaral, M. E. J.

doi:10.1111/age.12015

Cited by 5 publications

(4 citation statements)

References 35 publications

(31 reference statements)

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“…The major histocompatibility complex (MHC) is characterized by an intimate relationship between genes that play a role in resistance/susceptibility to infectious diseases, in addition to acting on the innate and adaptive immune response ( 11 ). In cattle, MHC (BoLA – bovine leukocyte antigen) is located on chromosome 23 ( 12 ) and its genes are grouped into three classes, I, II, and III, according to their function ( 13 ). Class II genes are the most studied and are divided into two separate regions of the chromosome.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing

Casa,

Giovambattista,

Fonteque

et al. 2023

Front. Vet. Sci.

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IntroductionThe BoLA-DRB3 gene in cattle is associated with tolerance to several infectious diseases, such as neosporosis, dermatophilosis, leukosis, and mastitis.MethodsThis study used PCR-SBT and BoLA-DRB3 gene sequencing to determine the association between the presence or absence of Anaplasma marginale, Babesia bovis, and Babesia bigemina infections in 208 Crioulo Lageano cattle and alleles present in the population. The chi-square test and odds ratio analysis were employed to establish the association.ResultsOf the BoLA-DRB3 gene alleles present in the population, two alleles were significantly associated with resistance to A. marginale infections: BoLA-DRB3001:01 (p < 0.001; OR = 0.224), which had a frequency of 7.93%, and BoLA-DRB3024:06 (p = 0.007; OR < 0.00001), which had a frequency of 0.72%. Regarding B. bovis infection, the BoLA-DRB3*011:01 allele (p = 0.002; OR = 0.271) had a frequency of 6% in the population and was associated with resistance to the infection. None of the alleles was associated with resistance to infection by B. bigemina.DiscussionThe Crioulo Lageano breed has alleles that may confer resistance against infection by A. marginale and B. bovis.

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Section: Introductionmentioning

confidence: 99%

“…Class II genes are the most studied and are divided into two separate regions of the chromosome. These two parts are known as IIa and IIb ( 12 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing

Casa,

Giovambattista,

Fonteque

et al. 2023

Front. Vet. Sci.

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“…Class II genes are separated into two subtypes designated class IIa and class IIb. Class IIa genes are closely associated with class I and class III regions, while class IIb genes are positioned closer to the centromere (Stafuzza et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

Stafuzza¹,

Olivatto²,

Naressi³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The DRB3 gene is an MHC class II gene that has a high degree of polymorphism with more than 100 alleles described in cattle. This variation contributes to differences among individuals in immune responsiveness and disease resistance. In this study, we searched for allelic variants in exon 2 of the DRB3 gene in 80 river buffaloes of three breeds in Brazil using a PCR-RFLP technique. The PCR product showed genetic polymorphism when digested with RsaI, PstI or HaeIII restriction enzymes. In total, 16 restriction patterns were identified: nine restriction patterns and 16 genotypes were found with RsaI; four restriction patterns and nine genotypes were found with HaeIII; and, three restriction patterns and four genotypes were found with PstI. Three RFLP patterns were exclusive to Jafarabadi buffaloes (RsaI-b, RsaI-c and RsaI-f) and three others were only observed in Mediterranean buffaloes (RsaI-g, RsaI-h and PstI-y). Jafarabadi buffaloes had a larger number of RFLP patterns than Mediterranean and Murrah breeds. The analysis showed that the DRB3 exon 2 was highly polymorphic, with the highest degree of polymorphism in Mediterranean buffaloes. This study provides the first assessment of allelic variation among three different buffalo breeds from Brazil and provides a basis for further investigations into the association between the DRB3 alleles and disease resistance.

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“…(d) to precisely anchor linkage and RH maps (Stafuzza et al 2013), as well as genome sequence contigs to specific chromosome regions (Goldammer et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Fluorescentin situhybridization mapping of three fecundity genes on cattle, river buffalo, sheep and goat

et al. 2015

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One of the goals of molecular cytogenetics applied to livestock is the extension of their genetic physical maps, especially of loci containing genes related to productions. In this study, a comparative fluorescence in situ (FISH) mapping of three genes related to fecundity of cattle, river buffalo, sheep and goat is reported using bovine BAC-clones taking in account the data available on the BovMap database and considering their physical position and the data obtained from banding experiments. The following three gene sequences were mapped: tumor necrosis factor-α (TNF), correlated to male fertility; signal transducer and activator of transcription 5A (STAT5A), important for its influence on milk production and reproduction activity; melatonin receptor 1A (MTNR1A) important for reproductive seasonality. BAC probes containing these gene sequences were assigned by FISH, for the first time, on RB-banded chromosomes of these four important bovids. TNF was assigned to BTA/CHI23q21-22, OAR20q21-22 and BBU 2p21-22; STAT5A was assigned to BTA/CHI19q17-21, OAR11q17-21 and BBU3p15-21; MTNR1A was assigned to BTA/CHI27q14-15, OAR26q14-15 and BBU1p21-22. The three loci were located in homoeologous chromosomes and chromosome bands, underling the high degree of chromosome homologies among Bovids and extending the cytogenetic maps of this economically important species.

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A high-resolution radiation hybrid map of the river buffalo major histocompatibility complex and comparison with BoLA

Cited by 5 publications

References 35 publications

Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing

Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing

Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

Fluorescentin situhybridization mapping of three fecundity genes on cattle, river buffalo, sheep and goat

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