Fluorescent<i>in situ</i>hybridization mapping of three fecundity genes on cattle, river buffalo, sheep and goat

Iannuzzi, Alessandra; Perucatti, A.; Pauciullo, Alfredo; Genualdo, V.; Lorenzi, L. De; Parma, P.; Iannuzzi, L.

doi:10.1080/00087114.2014.996038

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…However, FISH can be used in animals to localize genes of interest involved in fertility, milk and meat production, or other economically important traits. By using the FISH technique, three essential fecundity genes (TNF, STAT5A, and MTNR1A) were mapped on R-banded chromosomes of cattle, river buffalo sheep, and the goat for the first time, and extended the cytogenetic maps of this important species [67]. Tumor necrosis factor-α (TNF-α) is important for male fertility [68,69]; signal transducer and activator of transcription 5A (STAT5A) is correlated to reproduction activity and milk production [70,71]; melatonin receptor 1A (MTNR1A) is essential for seasonality of reproduction [72,73].…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Multi-OMICS and Molecular Biology Perspective in Buffalo Genome

et al. 2021

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The bovine species buffalo was domesticated from its wild strain Bubalus arnee and is widely used livestock in southern Asia. There are two distinct types of Buffalo- the swamp buffalo (B. bubalis kerebau) and the river buffalo (B. bubalis bubalis), which diverged from the wild Asian water buffalo and then evolved in separate geographical regions. Several research studies performed on buffalo, like- characterization of trait-specific Single Nucleotide Polymorphism (SNP), genetic and phenotypic diversity, gene prediction and function annotation, mapping of the draft genome, have helped our understanding of the buffalo genome. Some advanced discovery as identification of Single Nucleotide Variant (SNVs), Simple Sequence Repeats (SSR) marker and their association with various phenotypic traits, MicroRNA's expression profiling, whole-genome sequencing, etc. have also enabled us to track the chromosomal evolution, physiological processes, and gene expression of buffalo. Proper enhancement of these traits can lead us to apply multi-omics-based tools for better animal health and production. Recent advancement in genomic research on buffalo is being accelerated with the association of modern tools like- Genome-Wide Association Study (GWAS), genotyping by sequencing, epigenomic screening, microRNA's expression profiling, microarray technology, and whole-genome sequencing. All these tools bear great significance in breed up-gradation, identification of the phylogenetic relationship between species in proteome and genomic level, study gene expression level, diagnose diseases or developmental stages, phenotypic diversity, etc. All this knowledge paved the way for better optimization of production efficiency, product quality, and resistance to certain health hazards.

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Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Multi-OMICS and Molecular Biology Perspective in Buffalo Genome

et al. 2021

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Identification of Some Errors in the Genome Assembly of Bovidae by FISH

Lorenzi¹,

Parma²

2020

Cytogenet Genome Res

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From an economic point of view, Bovidae represent the most important family of the Ruminantia suborder. Thus, the mitochondrial and nuclear genomes of Bos taurus were among the first genomes to be sequenced after the sequencing of the human genomes. Over the millennia, the evolution of the genomes of the 3 main species belonging to the Bovidae family - B. taurus (BTA), Ovis aries (OAR), and Capra hircus (CHI) - has led to few chromosome rearrangements. Certainly, the availability and free access to the animal genomes significantly contributed to the improvement of animal genetics; however, some errors may exist due to the high automation in the genomic assembly construction process. In this work, some differences between the genomes of cattle, goat, and sheep highlighted by bioinformatics analysis have been verified by FISH, confirming that some errors persist even in the most recent genome assemblies. This type of approach has allowed us to detect a misassembly of a region belonging to BTA16 and to the homologues OAR12 and CHI16, a misassembly of a short tract in BTA22, OAR19, and CHI22, an incorrect mapping of a region of BTA21 and of CHI27 and OAR26, a discrepancy in the BTA26, OAR22, and CHI26 assemblies, a missed inversion in CHI1 compared to BTA1 and OAR1, and the exact assembly of a region of about 7 Mb in OAR10 and CHI12. Incorrect positioning of genomic tracts can cause unintended consequences in genetic analyses, especially when the data represent a starting point for the construction of genetic tools. In the new genomic assemblies published after the conclusion of our experiments, however, the accuracy in the construction of animal assemblies has been much improved, even if the new assemblies present more extended unmapped portions than the previous versions. The gap could be filled by comparative analyses between similar species or FISH.

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Fluorescentin situhybridization mapping of three fecundity genes on cattle, river buffalo, sheep and goat

Cited by 2 publications

References 26 publications

Multi-OMICS and Molecular Biology Perspective in Buffalo Genome

Multi-OMICS and Molecular Biology Perspective in Buffalo Genome

Identification of Some Errors in the Genome Assembly of Bovidae by FISH

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