A high-performance VEGF aptamer functionalized polypyrrole nanotube biosensor

Kwon, Oh Seok; Park, Seon Joo; Jang, Jyongsik

doi:10.1016/j.biomaterials.2010.02.040

Cited by 134 publications

(110 citation statements)

References 30 publications

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“…Kown et al previously reported a VEGF-detection system using aptamer-conjugated nanotube 12 with a 400 fM lower detection limit. Zhao et al reported another detection system using conformational change of VEGF-binding aptamer with a 5 pM lower detection limit.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Vascular Endothelial Growth Factor with Aptamer Sandwich

Nonaka

Abe

2012

Electrochemistry

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We developed an electrochemical vascular endothelial growth factor (VEGF) detection system using two VEGFbinding aptamers. Sensitive detection systems of VEGF are highly required for cancer diagnosis. In this study, VEGF-A was detected using a sandwiching method with a pyrroquinoline quinone glucose dehydrogenase (PQQ-GDH)-labeled VEGF-binding aptamer and another aptamer immobilized onto a gold wire electrode. We evaluated various combinations of VEGF-binding aptamers to be employed in the process. In addition, we examined dose dependency for the electric current generated by PQQ-GDH, measured in the constructed VEGF detection system. Using this newly constructed system, we successfully detected VEGF 165 at 15 nM (M = mol dm −3 ) concentration.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Vascular Endothelial Growth Factor with Aptamer Sandwich

Nonaka

Abe

2012

Electrochemistry

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“…In particular, 1-D conducting polymer (CP) nanomaterials that have various advantages, such as facile functionalization 29,[46][47][48][49][50] , cost effectiveness 50 , and biocompatibility 46,51,52 , have been highlighted in various applications, including supercapacitors, solar cells, transistors, and sensors. Moreover, in the field of biosensors, 1-D CP nanomaterials integrated into sensing geometries, have demonstrated high-performance transducing capabilities due to their efficient charge transport along the long-axis direction 29,47,50 .…”

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confidence: 99%

Human dopamine receptor nanovesicles for gate-potential modulators in high-performance field-effect transistor biosensors

Kwon¹

2016

Intrinsic Act

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The development of molecular detection that allows rapid responses with high sensitivity and selectivity remains challenging. Herein, we demonstrate the strategy of novel bio-nanotechnology to successfully fabricate high-performance dopamine (DA) biosensor using DA Receptor-containing uniform-particle-shaped Nanovesicles-immobilized Carboxylated poly(3,4-ethylenedioxythiophene) (CPEDOT) NTs (DRNCNs). DA molecules are commonly associated with serious diseases, such as Parkinson's and Alzheimer's diseases. For the first time, nanovesicles containing a human DA receptor D1 (hDRD1) were successfully constructed from HEK-293 cells, stably expressing hDRD1. The nanovesicles containing hDRD1 as gate-potential modulator on the conducting polymer (CP) nanomaterial transistors provided high-performance responses to DA molecule owing to their uniform, monodispersive morphologies and outstanding discrimination ability. Specifically, the DRNCNs were integrated into a liquid-ion gated field-effect transistor (FET) system via immobilization and attachment processes, leading to high sensitivity and excellent selectivity toward DA in liquid state. Unprecedentedly, the minimum detectable level (MDL) from the field-induced DA responses was as low as 10 pM in real-time, which is 10 times more sensitive than that of previously reported CP based-DA biosensors. Moreover, the FET-type DRNCN biosensor had a rapid response time (,1 s) and showed excellent selectivity in human serum.

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“…21 However, this process occurs at an elevated rate in tumor tissue and is related to proliferation and metastasis of cancer cells. 21 Vascular endothelial growth factor (VEGF) is a potent proangiogenic agent that is pivotal to the angiogenic process, and thus an attractive target for cancer therapy, as well as a useful means for early cancer detection. 21 Current methods for the detection and quantification of VEGF include enzyme-linked immunosorbent assay, VEGF receptors, and field-effect transistor biosensors.…”

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confidence: 99%

“…21 Vascular endothelial growth factor (VEGF) is a potent proangiogenic agent that is pivotal to the angiogenic process, and thus an attractive target for cancer therapy, as well as a useful means for early cancer detection. 21 Current methods for the detection and quantification of VEGF include enzyme-linked immunosorbent assay, VEGF receptors, and field-effect transistor biosensors. 21 There, however, exists room for improvement in the sensitivity and ease of use of these tests.…”

mentioning

confidence: 99%

“…21 Current methods for the detection and quantification of VEGF include enzyme-linked immunosorbent assay, VEGF receptors, and field-effect transistor biosensors. 21 There, however, exists room for improvement in the sensitivity and ease of use of these tests. To this end, Kwon et al combined conducting polymer (polypyrrole) nanotubes with aptamers (nanosize molecules with a great degree of sensitivity and specificity for target molecules), bonded to the nanotubes, in a modified biosensor for the detection of VEGF.…”

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confidence: 99%

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Overview of the role of nanotechnological innovations in the detection and treatment of solid tumors

Frank¹,

Tyagi²,

Tomar³

et al. 2014

IJN

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Nanotechnology, although still in its infantile stages, has the potential to revolutionize the diagnosis, treatment, and monitoring of disease progression and success of therapy for numerous diseases and conditions, not least of which is cancer. As it is a leading cause of mortality worldwide, early cancer detection, as well as safe and efficacious therapeutic intervention, will be indispensable in improving the prognosis related to cancers and overall survival rate, as well as health-related quality of life of patients diagnosed with cancer. The development of a relatively new field of nanomedicine, which combines various domains and technologies including nanotechnology, medicine, biology, pharmacology, mathematics, physics, and chemistry, has yielded different approaches to addressing these challenges. Of particular relevance in cancer, nanosystems have shown appreciable success in the realm of diagnosis and treatment. Characteristics attributable to these systems on account of the nanoscale size range allow for individualization of therapy, passive targeting, the attachment of targeting moieties for more specific targeting, minimally invasive procedures, and real-time imaging and monitoring of in vivo processes. Furthermore, incorporation into nanosystems may have the potential to reintroduce into clinical practice drugs that are no longer used because of various shortfalls, as well as aid in the registration of new, potent drugs with suboptimal pharmacokinetic profiles. Research into the development of nanosystems for cancer diagnosis and therapy is thus a rapidly emerging and viable field of study.

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A high-performance VEGF aptamer functionalized polypyrrole nanotube biosensor

Cited by 134 publications

References 30 publications

Electrochemical Detection of Vascular Endothelial Growth Factor with Aptamer Sandwich

Electrochemical Detection of Vascular Endothelial Growth Factor with Aptamer Sandwich

Human dopamine receptor nanovesicles for gate-potential modulators in high-performance field-effect transistor biosensors

Overview of the role of nanotechnological innovations in the detection and treatment of solid tumors

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