A high‐performance multilane microdevice system designed for the DNA forensics laboratory

Goedecke, Nils; McKenna, Brian; El-Difrawy, Sameh; Carey, Loucinda; Matsudaira, Paul; Ehrlich, D. J.

doi:10.1002/elps.200305897

Cited by 61 publications

(48 citation statements)

References 14 publications

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“…More recently, a system supporting a glass chip with 16 lanes of 20 cm effective length and double-T cross injector was presented by Goedecke et al [103]. An absolute three-sigma peak accuracy of 0.4-0.9 bp was reported for the CODIS 13-locus multiplex, utilizing a single channel per sample.…”

Section: Forensic Dna and Rna Analysismentioning

confidence: 99%

Recent advances in the applications of CE to forensic sciences (2001–2004)

Tagliaro

Bortolotti

2006

Electrophoresis

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Recent advances in the applications of CE to forensic sciences (2001-2004)The present article reviews the applications of CE in forensic science covering the period from 2001 until the first part of 2005. The overview includes the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques in the following fields: (i) Forensic drugs and poisons, (ii) explosive analysis and gunshot residues, (iii) small ions of forensic interest, (iv) forensic DNA and RNA analysis, (v) proteins of forensic interest, and (vi) ink analysis.

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Section: Forensic Dna and Rna Analysismentioning

confidence: 99%

Recent advances in the applications of CE to forensic sciences (2001–2004)

Tagliaro

Bortolotti

2006

Electrophoresis

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“…Several DNA approaches has been performed on chip such as mutation analysis, SNPs analysis, forensic human identification, and genotyping, [55][56][57][58][59][60][61][62] therefore a sensitive, efficient and low viscous separation media has become increasingly important for DNA separation on chip. Below several low viscous separation media has been introduced for analysis on chip.…”

Section: Low Viscous Separation Medias For Dna Separationmentioning

confidence: 99%

“…[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Several types of chips has been introduced for the postgenomic researches such as DNA/RNA chips [28][29][30][31] and the serial analysis of gene expression. [32][33][34][35] The viscosity of the separation media is one important factor for chip separation since the bounded microfluidic devices can not withstand high loading pressure.…”

Section: Introductionmentioning

confidence: 99%

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

Jabasini

Murakami

Kaji

et al. 2006

Biological & Pharmaceutical Bulletin

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Microchip electrophoresis has widely grown during the past few years, and it has showed a significant result as a strong separation tool for genomic as well as proteomic researches. To enhance and expand the role of microchip electrophoresis, several studies have been proposed especially for the low viscous separation media, which is an important factor for the success of microchip with its narrow separation channels. In this paper we show an overview for the done researches in the field of low viscous media developed for the use in microchip electrophoresis. For genomic separation studies polyhydroxy additives have been used enhance the separation of DNA at low polymer concentration of HPMC (Hydroxypropylmethyl cellulose) which could keep the viscosity low. Mixtures of poly(ethylene oxide) as well as Hydroxyporpyl cellulose have been successfully introduced for chip separation. Furthermore high molecular mass polyacrylamides at low concentrations have been studied for DNA separation. A mixture of polymer nanoparticle with conventional polymers could show a better resolution for DNA at low concentration of the polymer. For the proteomic field isoelectric focusing on chip has been well overviewed since it is the most viscous separation media which is well used for the protein separation. The different types of isoelectric focusing such as the ampholyte-free type, the thermal type as well as the ampholyte-depended type have been introduced in this paper. Isoelectric focusing on chip with its combination with sodium dodecyl sulfate (SDS) page or free solution could give a better separation. Several application for this low viscous separation medias for either genomic or proteomic could clearly show the importance of this field.

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“…In the future, additional loci are likely to be added to routine forensic analysis. Forensic DNA fingerprinting utilizes a miniature system of electrophoretic columns to analyze the length of each of the 13 STR loci (5).…”

mentioning

confidence: 99%

Branch migration displacement assay with automated heuristic analysis for discrete DNA length measurement using DNA microarrays

Pourmand

Caramuta

Villablanca

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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The analysis of short tandem repeats (STRs) plays an important role in forensic science, human identification, genetic mapping, and disease diagnostics. Traditional STR analysis utilizes gel-or columnbased approaches to analyze DNA repeats. Individual STR alleles are separated and distinguished according to fragment length; thus the assay is generally hampered by its low multiplex capacity. However, use of DNA microarray would employ a simple hybridization and detection for field forensics and biology. Here we demonstrate a rapid, highly sensitive method for STR analysis that utilizes DNA microarray technology. We describe two adaptations to accomplish this: the use of competitive hybridization to remove unpaired ssDNA from an array and the use of neural network classification to automate the analysis. The competitive displacement technique mimics the branch migration process that occurs during DNA recombination. Our technique will facilitate the rapid deduction of identity, length, and number of repeats for the multiple STRs in an unknown DNA sample.DNA fingerprinting ͉ DNA profiling ͉ short tandem repeat

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A high‐performance multilane microdevice system designed for the DNA forensics laboratory

Cited by 61 publications

References 14 publications

Recent advances in the applications of CE to forensic sciences (2001–2004)

Recent advances in the applications of CE to forensic sciences (2001–2004)

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

Branch migration displacement assay with automated heuristic analysis for discrete DNA length measurement using DNA microarrays

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