A high-performance liquid chromatographic analysis of the benzoate esters of sapogenins isolated from Agave

Saponins of Agave: Chemistry and bioactivity

“…Results of the HPLC quantification of hecogenin (1) and tigogenin (2) in the leaves of A. sisalana were comparable to that obtained by GLC method (Sitton et al, 1982). Benzoyl esters of sapogenins of Agave were also analyzed by RP-HPLC (Higgins, 1976). Hyphenated techniques such as HPLC coupled with mass spectrometry (HPLC-MS or LC-MS), HPLC coupled with nuclear magnetic resonance spectroscopy (LC-NMR) and capillary electrophoresis coupled with mass spectroscopy (CE-MS) have also been used for the rapid screening of the plant extracts/fractions for the presence of saponins (Sarkar and Nahar, 2006;Hostettmann et al, 1996).…”

Production of diosgenin by hairy root cultures of Trigonella foenum-graecum L.

“…The selected procedure was based on the formation of the 3-benzoyl derivative essentially as described by Higgins (1976): 2 ml of dried pyridine (Merck, Germany) and 0.1 ml of benzoyl chloride (Fluka) were added to the dried extract. The sample was heated at 80°C for 30 min then, after addition of 2 ml of methanol, heated again at 100°C for 30 min.…”

Analysis of Spirostans, Spirosolanes, and Solanidanes

“…A quantitative method for the determination of the commercially important compound hecogenin (2a,12oxo,22a,25S) in Agave species has been reported (Higgins, 1976). After hydrolysis of the glycosides, the aglycones hecogenin and tigogenin (2a,22a,25R) were converted to the benzoates and separated on a C8 column, with ACN/H 2 0 (80:20) as the mobile phase at a relatively high flow rate of 3.9 ml/min and detection at 235 nm.…”