A high-frequency cyclic secondary somatic embryogenesis system for Cyclamen persicum Mill

You, Cui Rong; Fan, Ting Jun; Gong, Xue; Bian, Fu Hua; Li, Liang; Qu, Fu Ning

doi:10.1007/s11240-011-9974-y

Cited by 30 publications

(16 citation statements)

References 33 publications

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“…Aplikasi auksin yang dikombinasikan dengan sitokinin sering ditambahkan ke dalam media untuk menginduksi baik kalus maupun embrio somatik dengan gradasi konsentrasi auksin. Auksin yang sering digunakan adalah 2,4-D karena daya induksinya lebih kuat dan harganya lebih murah dibanding dengan jenis auksin lainnya (Alkhateeb, 2006;Boufis et al, 2014;Cai et al, 2009;Hajong et al, 2013;MunozConcha et al, 2011;You et al, 2011). Penelitian embriogenesis somatik tanaman sagu telah berhasil dikembangkan oleh Tahardi et al (2002) dengan menggunakan sistem kultur media padat.…”

Section: Pendahuluanunclassified

“…Hal ini sesuai dengan hasil penelitian induksi dan proliferasi SSE pada tanaman Cyclamen persicum yang dapat mencapai 3.306 embrio/g bobot segar dengan penambahan 2,4-D 2 mg/l dikombinasikan dengan 0,2 mg/l BA (You et al, 2011). ZPT 2,4-D yang dikombinasikan dengan kinetin pada penelitian ini tampaknya cukup untuk memacu proliferasi kalus dan pembentukan embrio somatik primer yang diikuti dengan SEE sehingga produksi embrio somatik lebih banyak.…”

Section: Induksi Embriogenesis Somatikunclassified

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Proliferasi Kalus dan Induksi Embriogenesis Somatik Tanaman Sagu (Metroxylon saguRottb.) Menggunakan Tiga Metode Kultur: Sistem Perendaman Sesaat, Suspensi dan Media Padat

et al. 2018

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The accurate method of in vitro culture will increase the effectiveness and efficiency of sago palm callus proliferation and somatic embryogenesis induction. The research was conducted to evaluate the effectiveness of three methods for tissue culture, i.e. suspension, temporary immersion system (TIS), and solid media culture, for indirect somatic embryogenesis of sago palm "Alitir" derived from Merauke, Papua. A clump of friable calli derived from sucker's tip meristem culture was used as starting material. The calli were cultured on a modified Murashige and Skoog medium with 5.0-15.0 mg/l 2,4-D combined with 0.1 mg/l kinetin using three culture methods. There were twelve treatment combinations. The results showed that the highest fresh weight of calli was 12.0 g/flask achieved by suspension culture method supplemented with 15 mg/l 2.4-D combined with 0.1 mg/l kinetin. The highest number of somatic embryo was 384.7 pcs/flask achieved by suspension culture method with supplemented 5 mg/l 2.4-D combined with 0.1 mg/l kinetin. The best survival rate (100%) was obtained by suspension culture method for all treatments with 2.4-D concentration. During the somatic embryo induction of sago palm, the color of calli has changed from mostly yellowish to light yellow and yellowish-white.Keywords: Calli proliferation, culture method, Metroxylon sagu Rottb., plant growth regulator, somatic embryo induction. ABSTRAKMetode kultur in vitro yang tepat akan meningkatkan efektivitas dan efisiensi pada proses penggandaan kalus dan induksi embriogenesis somatik. Penelitian ini bertujuan mengevaluasi efektivitas tiga metode kultur jaringan, yaitu sistem kultur suspensi, sistem perendaman sesaat (SPS) atau temporary immersion system (TIS), dan media padat, untuk proliferasi kalus dan pembentukan embrio somatik secara tidak langsung pada tanaman sagu "Alitir" yang berasal dari Merauke, Papua. Bahan tanaman atau eksplan awal yang digunakan adalah kalus remah hasil induksi dari kultur meristem pucuk tunas anakan sagu. Kalus tersebut dikulturkan pada media Murashige dan Skoog (MS) modifikasi dengan penambahan 2,4-D 5,0-15,0 mg/l dikombinasikan dengan kinetin 0,1 mg/l menggunakan ketiga metode kultur sehingga terdapat dua belas kombinasi perlakuan. Hasil penelitian menunjukkan bobot segar kalus tertinggi sebesar 12,0 g/bejana dicapai pada metode kultur suspensi dengan penambahan 2,4-D 15,0 mg/l dikombinasikan dengan kinetin 0,1 g/l. Perolehan jumlah embrio somatik tertinggi dicapai pada metode kultur suspensi dengan penambahan 2,4-D 5,0 mg/l dikombinasikan dengan kinetin 0,1 g/l sebesar 384,7 buah/bejana. Daya hidup kultur sagu terbaik dan tertinggi (100%) diperoleh pada metode kultur suspensi pada semua perlakuan konsentrasi 2,4-D. Selama proses induksi embrio somatik, terjadi perubahan warna kalus dari sebagian besar kekuningan menjadi krem dan putih-kekuningan.Kata kunci: Induksi embrio somatik, metode kultur, Metroxylon sagu Rottb., zat pengatur tumbuh, proliferasi kalus.Hak Cipta © 2016, BB Biogen Jurnal AgroBiogen 12(1):37-44

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Section: Pendahuluanunclassified

Section: Induksi Embriogenesis Somatikunclassified

Proliferasi Kalus dan Induksi Embriogenesis Somatik Tanaman Sagu (Metroxylon saguRottb.) Menggunakan Tiga Metode Kultur: Sistem Perendaman Sesaat, Suspensi dan Media Padat

et al. 2018

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“…Daya hidup yang tinggi dapat mendorong peningkatan biomassa dan jumlah induksi embrio somatik sehingga pada saat panen dapat menghasilkan bobot basah maupun jumlah embrio somatik tertinggi (Gambar 1 & 2). Hal ini mirip pada hasil penelitian yang dilakukan oleh You et al (2011) mengenai embriogenesis somatik pada tanaman Cyclamen persicum Mill. Hasil penelitiannya menunjukkan bahwa persentase hidup kultur yang tinggi mendorong peningkatan biomassa sehingga saat panen dihasilkan planlet yang lebih banyak.…”

Section: Daya Hidup Kulturunclassified

Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods)

Riyadi¹,

Efendi²,

Purwoko³

et al. 2018

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“…One possible reason for low callus induction may be the recalcitrance of wild or endemic species, supported by evidence in the literature, as follows next. For example, You et al (2011) noted that some C. persicum cultivars were recalcitrant to somatic embryogenesis. C. persicum showed very low levels of somatic embryogenesis when mature tissues or organs were used as explants Schmidt et al 2006).…”

Section: Callus Induction From Different Explant Types and Pgrs Of Enmentioning

confidence: 99%

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

İzgü

Sevindik

Çürük

et al. 2016

Plant Cell Tiss Organ Cult

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In this study, embryo-like structures (ELSs) were induced in four endemic Turkish Cyclamen species (C. cilicium Boiss. et Heldr., C. parviflorum Pobed., C. mirabile Hildebr. and C. pseudibericum Hildebr.) in the presence of 13 combinations of two plant growth regulators (PGRs) (2,4-dichlorophenoxyacetic acid and 6-(c,cdimethylallylamino)purine) and four explant types (ovules, ovaries, leaves and petioles). The ratio of callus induction, different stages of ELS formation and the conversion of ELSs to plantlets were quantified. The most effective explant types for callus induction were leaves (56 % for C. cilicium and 59 % for C. parviflorum) and petioles (80 % for C. mirabile and 100 % for C. pseudibericum). Callus growth from the leaves and petioles of C. cilicium was 30 days earlier than that of C. mirabile and C. pseudibericum. In contrast, most callus formed from the petioles of C. pseudibericum (100 %) in medium with 2.5 mg l -1 2,4-D and 1 mg l -1 2iP. The highest number of ELSs was obtained succesfully from petioles (2.5 mg l -1 2,4-D and 1 mg l -1 2iP) and ovaries (2.5 mg l -1 2,4-D and 0.5 mg l -1 2iP) of C. pseudibericum, in 39 % and as 32 % of explants, respectively. The percentage conversion of ELSs to plantlets was 38, 31, 16 and 15 % for C. mirabile, C. cilicium, C. pseudibericum and C. parviflorum, respectively. The plantlets were successfully acclimatized in the greenhouse with 54, 70, 63 and 25 % of C. cilicium, C. mirabile, C. pseudibericum and C. parviflorum plantlets, respectively surviving after transfer to ex vitro conditions. This paper describes a unique, reliable and consistent protocol for the induction of ELSs from four endangered endemic Turkish Cyclamen species, opening up the possibility of preserving these valuable genetic resources in vitro and also other applied biotechnologies that rely on a stable embryogenic or callus-based protocol.

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A high-frequency cyclic secondary somatic embryogenesis system for Cyclamen persicum Mill

Cited by 30 publications

References 33 publications

Proliferasi Kalus dan Induksi Embriogenesis Somatik Tanaman Sagu (Metroxylon saguRottb.) Menggunakan Tiga Metode Kultur: Sistem Perendaman Sesaat, Suspensi dan Media Padat

Proliferasi Kalus dan Induksi Embriogenesis Somatik Tanaman Sagu (Metroxylon saguRottb.) Menggunakan Tiga Metode Kultur: Sistem Perendaman Sesaat, Suspensi dan Media Padat

Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods)

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

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