A high-density poly(ethylene glycol) polymer brush for immobilization on glass-type surfaces

Piehler, Jacob; Brecht, Andreas; Valiokas, Ramūnas; Liedberg, Bo; Gauglitz, Günter

doi:10.1016/s0956-5663(00)00104-4

Cited by 229 publications

(220 citation statements)

References 27 publications

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“…The silanization has been extensively studied in literature, and was therefore not further investigated. [142,[149][150] However, up-scaling of the reaction to coat the 22 x 21 cm² laser printer slides is still an on-going process. So far, only "lower quality" coatings could be achieved even if higher concentrations of 3-GPS in the silanization step were applied.…”

Section: Discussionmentioning

confidence: 99%

“…In a SPOT synthesis approach, AST et al pre-modified a cellulose sheet with epibromohydrin and opened the resulting epoxy group with 1,13-diamino-4,7,10-trioxatridecane (DATT) to obtain a homogenous amino coating. [141] Using a bifunctional compound to open the epoxy rings of a 3-(glycidyl)oxypropyl trimethoxysilane (3-GPS) SAM was extensively studied by PIEHLER et al and SALES et al [142][143] The technique has been further developed by MEHNE et al to obtain PEG-NH2 SAMs of mixed chain length for reflectometric interference spectroscopy (RIfS) in the label-free detection of biomolecules. [144] Hence, the synthesis supports for the peptide array synthesis should be silanized with 3-GPS (Figure 13, a) and, then, reacted with DATT to give an amino terminated SAM (Figure 13, b).…”

Section: Initial Considerationsmentioning

confidence: 99%

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Purification of Peptides in High-Complexity Arrays

Schirwitz

2013

Springer Theses

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Compared to the human genome, which is formed by approximately 25,000 genes, the human proteome comprises over a million functional proteins and thus represents a much higher diversity. Although genetic research provides valuable information on the proteins which can be translated, a great task in the future will be the exploration of the entire protein interaction network. Understanding protein interactions will greatly help scientists to identify the mechanisms behind fatal diseases such as cancer, AIDS, and tuberculosis and, hopefully, provide new

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Section: Discussionmentioning

confidence: 99%

Section: Initial Considerationsmentioning

confidence: 99%

Purification of Peptides in High-Complexity Arrays

Schirwitz

2013

Springer Theses

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“…Since a passivated titanium surface exhibits similar surface chemistry to that of silica [12], a bifunctional linker, glycidoxypropyltrimethoxysilane (GOPTS), was used as the linking molecule [13,141. GOPTS presents, on one end, a trialkyloxysilane group, like APS, which allows its incorporation on activated silica or silica-like surfaces. Once GOPTS is deposited on a surface, it exposes epoxy moieties which are unstable and therefore very reactive toward nucleophilic groups such as amines, thiols, and alcohols [14]. These groups will promptly react causing opening of the bond angle strained epoxide ring.…”

Section: Methodsmentioning

confidence: 99%

Real-Time Salmonella Detection Using Lead Zirconate Titanate-Titanium Microcantilevers

et al. 2004

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Current methods for analysis of unknown powders in suspicious packages involve sending samples to laboratory facilities where a variety of time-consuming tests are performed. We have developed and investigated the use of a lead zirconate titanate -titanium (PZT-Ti) microcantilever for in situ detection of the common food-and water-born pathogen, Salmonella typhimurium. Using a bifunctional linking molecule to immobilize antibody on the titanium surface of the microcantilever, we can directly detect salmonella cells in suspensions of differing concentration. This novel surface functionalization technique along with the sub-nanogram sensitivity of the cantilever has allowed for direct quantification of S. typhimurium cells in suspension.

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“…Microarray coatings of type 2B can be prepared using a variety of chemically different linker systems including glutaraldehyde ("aldehyde slides"), phenylendiisothiocyanate, PEGs of different molar mass, or various heterobifunctional crosslinker [7,22,[25][26][27][28][29][30][31]. To date, only a few of these slide surfaces are commercially available.…”

Section: Molecular Architecturementioning

confidence: 99%

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Sobek¹,

Bartscherer²,

Jacob³

et al. 2006

CCHTS

110

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Over the last years microarray technology has become one of the principal platform technologies for the highthroughput analysis of biological systems. Starting with the construction of first DNA microarrays in the 1990s, microarray technology has flourished in the last years and many different new formats have been developed. Peptide and protein microarrays are now applied for the elucidation of interaction partners, modification sites and enzyme substrates. Antibody microarrays are envisaged to be of high importance for the high-throughput determination of protein abundances in translational profiling approaches. First cell microarrays have been constructed to transform microarray technology from an in vitro technology to an in vivo functional analysis tool. All of these approaches share a common prerequisite: the solid support on which they are generated. The demands on this solid support are thereby as manifold as the applications themselves. This review is aimed to display the recent developments in surface chemistry and derivatization, and to summarize the latest developments in the different application areas of microarray technology.

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A high-density poly(ethylene glycol) polymer brush for immobilization on glass-type surfaces

Cited by 229 publications

References 27 publications

Purification of Peptides in High-Complexity Arrays

Purification of Peptides in High-Complexity Arrays

Real-Time Salmonella Detection Using Lead Zirconate Titanate-Titanium Microcantilevers

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

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