A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.)

Abstract

A microsatellite consensus map was constructed by joining four independent genetic maps of bread wheat. Three of the maps were F(1)-derived, doubled-haploid line populations and the fourth population was 'Synthetic' x 'Opata', an F(6)-derived, recombinant-inbred line population. Microsatellite markers from different research groups including the Wheat Microsatellite Consortium, GWM, GDM, CFA, CFD, and BARC were used in the mapping. A sufficient number of common loci between genetic maps, ranging from 52 to 232 loci, were mapped on different populations to facilitate joining the maps. Four genetic maps were developed using MapMaker V3.0 and JoinMap V3.0. The software CMap, a comparative map viewer, was used to align the four maps and identify potential errors based on consensus. JoinMap V3.0 was used to calculate marker order and recombination distances based on the consensus of the four maps. A total of 1,235 microsatellite loci were mapped, covering 2,569 cM, giving an average interval distance of 2.2 cM. This consensus map represents the highest-density public microsatellite map of wheat and is accompanied by an allele database showing the parent allele sizes for every marker mapped. This enables users to predict allele sizes in new breeding populations and develop molecular breeding and genomics strategies.

A high density barley microsatellite consensus map with 775 SSR loci

“…Further, dense genetic maps are important to prepare contig-based local or genome wide physical maps, for map-based cloning and for genome sequencing projects. Since microsatellite markers are currently preferred over other molecular markers for a variety of reasons, high density microsatellite maps, such as those developed in rice (McCouch et al 2002), maize (Sharopova et al 2002) and wheat (Somers et al 2004), are very useful.…”

AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides × T. tauschii) as a source of favourable alleles for milling and baking quality traits

“…pw.usda.gov. The chromosomal positions of the SSR markers were obtained from the consensus map of Somers et al (2004) or estimated from flanking markers of the genetic map of R. Ward (2003, http://www.scabusa.org). The genotyped markers were assigned to bins according to information by Sourdille et al (2004).…”

“…An asterisk (*) indicates exotic QTL alleles showing a favourable effect. The map is based on Somers et al (2004) Falling number (FN) For the trait FN, five putative QTLs were found in B22. Of these QTLs, two loci showed an increase in trait performance by 11.7-19.6%, if the exotic allele was present.…”

Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

“…This QTL appeared to have a more consistent eVect than the other minor QTL. This locus is near marker Xbarc55 (Somers et al 2004), which was reported to be linked to the QTL in cultivar Clark Cream (http://maswheat.ucdavis.edu/protocols/PHS). Thus, it is possible that they share the same locus.…”

“…The two bulks consisted of Wve highly PHS-resistant RILs and Wve highly PHS-sensitive RILs, respectively, from the RIL population of Rio Blanco/ NW97S186. A total of 1,430 SSR primer pairs were screened between parents and bulks, which included BARC (Song et al 2005), GWM (Röder et al 1998), WMC (Somers et al 2004), GDM (Pestsova et al 2000), CFA, CFD (Guyomarc'h et al 2002;Sourdille et al 2003), DUP (Eujayl et al 2002), and KSM primers developed at Kansas State University, Manhattan, KS, USA. Polymorphic markers between the bulks were analyzed further in the RIL population.…”