A high density barley microsatellite consensus map with 775 SSR loci

Varshney, Rajeev K.; Marcel, Thierry C.; Ramsay, Luke; Russell, Joanne; Röder, Marion S.; Stein, Nils; Waugh, Robbie; Langridge, Peter; Niks, Rients E.; Graner, Andreas

doi:10.1007/s00122-007-0503-7

Cited by 303 publications

(248 citation statements)

References 49 publications

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“…In our study, two significant QTL with effect on SL in all 3 years were found. Examining the 2H and 7H linkage maps of Varshney et al (2007) revealed that Qsl2-15 is close to the centromere of the 2HL, and is different from those QTL on chromosomes 2H previously reported (Hori et al, 2003;Li et al, 2005;Sameri et al, 2006;Wang et al, 2010). Qsl7-7 is likely the same as Qel7.1 on chromo-some 7H previously reported (Li et al, 2005).…”

Section: Qtl For Plant Height Componentscontrasting

confidence: 69%

“…QTL conferring internode length are reported to be linked with the genes vrs1 (2HL), Ppd-H1 (2HS), uzu (3HL), sgh1 (4HL), Ema5 (5HL), sgh2 (5HL), qCUL.ak-7H (7HL) and dsp1 (7HS) in barley (Sameri et al, 2006(Sameri et al, , 2009. Examining the 2H, 3H, 5H, 6H, and 7H linkage maps of Varshney et al (2007) and GrainGenes2.0 (http://wheat.pw.usda.gov) revealed that the location of Qion3-9 is close to the gene uzu on chromosome 3HL, and thirteen QTL for the internode length are different from those QTL reported by Sameri et al (2006Sameri et al ( , 2009. In our study, all seven plant height components showed a highly significant positive correlation with each other (Table 3).…”

Section: Qtl For Plant Height Componentsmentioning

confidence: 99%

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Molecular detection of QTL controlling plant height components in a doubled haploid barley population

Ren¹,

Sun²,

Dong³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Yield losses caused by lodging in barley can be partially controlled by reducing plant height. In order to understand dwarfing mechanisms and efficiently use new dwarf germplasms for a breeding program, it is important to identify QTL of plant height components. QTL analysis was performed for seven plant height component traits using a DH population of 122 lines derived from the cross of Huaai 11 x Huadamai 6. Composite interval mapping procedures detected 20 QTL, which were mapped onto chromosomes 2H, 3H, 5H, 6H, and 7H. Eleven QTL were detected in 3 years and four QTL were detected in 2 years. QTL controlling all seven plant height component traits were found near the dwarfing gene btwd1 on chromosome 7H. These QTL accounted for 27.19 to 59.73% of phenotypic variation in seven plant height component traits. Positive transgressive segregation was found for all traits. Some of the QTL identified in this study will be useful for understanding the dwarfing mechanism and for developing new dwarf varieties using marker-assisted selection.

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Section: Qtl For Plant Height Componentscontrasting

confidence: 69%

Section: Qtl For Plant Height Componentsmentioning

confidence: 99%

Molecular detection of QTL controlling plant height components in a doubled haploid barley population

Ren¹,

Sun²,

Dong³

et al. 2014

Genet. Mol. Res.

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“…For the purposes of this analysis, an initial screening was performed by using the 14 SSR markers selected from the work of Varshney et al (8). SSR markers were amplied and their PIC values assessed using a subset of samples belonging to both the local and the commercial varieties.…”

Section: Discussionmentioning

confidence: 99%

“…The set of 14 SSR marker loci investigated in this study was obtained from Varshney et al (8). An initial screening of a subset of 12 DNA samples, randomly cho- °C.…”

Section: Analysis Of Ssr Markersmentioning

confidence: 99%

“…Information on the simple sequence repeat (SSR) marker loci analysed in this study, including linkage group, locus name, primer sequence and amplicon size. The polymorphism information content (PIC) value is also reported for each locus (8). Loci written in bold were the ones assayed in this study The genetic structure of the Agordino landrace and of the entire barley core collection based on a total of 95 DNA samples, including the individuals of the local population and commercial materials (i.e.…”

Section: Marker Analysismentioning

confidence: 99%

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Developing a Molecular Identification Assay of Old Landraces for the Genetic Authentication of Typical Agro-Food Products: the Case Study of the Barley ‘Agordino’

Palumbo¹,

Galla²,

Barcaccia³

2017

Food Technol. Biotechnol.

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SummaryThe orzo Agordino is a very old local variety of domesticated barley (Hordeum vulgare ssp. distichum L.) that is native to the Agordo District, Province of Belluno, and is widespread in the Veneto Region, Italy. Seeds of this landrace are widely used for the preparation of very famous dishes of the dolomitic culinary tradition such as barley soup, bakery products and local beer. Understanding the genetic diversity and identity of the Agordino barley landrace is a key step to establish conservation and valorisation strategies of this local variety and also to provide molecular traceability tools useful to ascertain the authenticity of its derivatives. The gene pool of the Agordino barley landrace was reconstructed using 60 phenotypically representative individual plants and its genotypic relationships with commercial varieties were investigated using 21 pure lines widely cultivated in the Veneto Region. For genomic DNA analysis, following an initial screening of 14 mapped microsatellite (SSR) loci, seven discriminant markers were selected on the basis of their genomic position across linkage groups and polymorphic marker alleles per locus. The genetic identity of the local barley landrace was determined by analysing all SSR markers in a single multi-locus PCR assay. Extent of genotypic variation within the Agordino barley landrace and the genotypic di erentiation between the landrace individuals and the commercial varieties was determined. Then, as few as four highly informative SSR loci were selected and used to develop a molecular traceability system exploitable to verify the genetic authenticity of food products deriving from the Agordino landrace. This genetic authentication assay was validated using both DNA pools from individual Agordino barley plants and DNA samples from Agordino barley food products. On the whole, our data support the usefulness and robustness of this DNA-based diagnostic tool for the orzo Agordino identi cation, which could be rapidly and e ciently exploited to guarantee the authenticity of local varieties and the typicality of food products.

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Significance, Adaptation, Production, and Trade of Barley

Ullrich

2010

Barley

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A high density barley microsatellite consensus map with 775 SSR loci

Cited by 303 publications

References 49 publications

Molecular detection of QTL controlling plant height components in a doubled haploid barley population

Molecular detection of QTL controlling plant height components in a doubled haploid barley population

Developing a Molecular Identification Assay of Old Landraces for the Genetic Authentication of Typical Agro-Food Products: the Case Study of the Barley ‘Agordino’

Significance, Adaptation, Production, and Trade of Barley

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