A heteropolysaccharide purified from leaves of Ilex latifolia displaying immunomodulatory activity in vitro and in vivo

Shi, Zhaoyu; An, Li; Zhang, Shaojie; Li, Zhengguo; Li, Yuhao; Cui, Jianlin; Zhang, Jie; Jin, Da-Qing; Tuerhong, Muhetaer; Abudukeremu, Munira; Xu, Jing; Guo, Yuanqiang

doi:10.1016/j.carbpol.2020.116469

Cited by 28 publications

(13 citation statements)

References 46 publications

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“…In the aggregated state, an irregular branch network structure with an uneven surface was noted, suggesting an amorphous structure of GFPA. The surface of GFPA was uneven in thickness and showed crests and sags, which may have been caused by the branched structure of GFPA ( Figures 2G–J ) ( 31 ).…”

Section: Resultsmentioning

confidence: 99%

The anti-obesity effects of a water-soluble glucan from Grifola frondosa via the modulation of chronic inflammation

et al. 2022

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Polysaccharides from Grifola frondosa (G. frondosa) have anti-obesity and anti-inflammatory activities. In this study, the major type, molecular weight, homogeneity and structure of a polysaccharide purified from G. frondosa (denoted GFPA) were determined. In high-fat diet (HFD)-treated mice, 8 weeks of GFPA administration efficiently decreased body weight and blood glucose concentration and counteracted hyperlipidemia. GFPA efficiently decreased adipocyte size and ameliorated inflammatory infiltration in the three types of white adipose tissue and alleviated steatosis, fat accumulation and inflammatory infiltration in the livers of HFD-fed mice. GFPA also decreased the concentrations of aspartate aminotransferase, alanine aminotransferase and pro-inflammatory factors in the sera and livers of HFD-treated mice. Furthermore, GFPA was found to regulate lipid metabolism via the inhibition of ceramide levels in HFD-treated mice. GFPA exhibited strong anti-obesity effects via the modulation of chronic inflammation through Toll-like receptor 4/nuclear factor kappa-B signaling, which supports the use of GFPA for the treatment of obesity.

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Section: Resultsmentioning

confidence: 99%

The anti-obesity effects of a water-soluble glucan from Grifola frondosa via the modulation of chronic inflammation

et al. 2022

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“…Shi et al . (2020) suggested that Ilex latifolia Thunb polysaccharide (ILP50‐2) could significantly promote the secretion of NO and ROS in zebrafish, which exhibited immunoregulatory effects in vivo . In the present study, we only evaluated the in vitro immunomodulatory activity of PGPIV‐1‐a on macrophages.…”

Section: Resultsmentioning

confidence: 99%

“…Effects of PGPIV-1-a on NO, TNF-a and IL-6 production of RAW 264.7 cells Figure 5 Triple-helical conformation analysis of PGPIV-1-a. When macrophages are activated, they can induce the production of NO and cytokine (TNF-a and IL-6), which plays indispensable roles in immunomodulatory responses (Sun et al, 2015). Nitric oxide (NO) is an intracellular messenger, which contributes to defend the host against the invasion of pathogens and mediates a variety of biological functions (Du et al, 2016).…”

Section: Immunomodulatory Activity Of Pgpiv -1-amentioning

confidence: 99%

“…Gong et al (2020) found that PHPD-IV-4, a polysaccharide isolated from Porphyra haitanensis, induced macrophages activation by up-regulating the phosphorylation of MAPK signalling molecules. Shi et al (2020) suggested that Ilex latifolia Thunb polysaccharide (ILP50-2) could significantly promote the secretion of NO and ROS in zebrafish, which exhibited immunoregulatory effects in vivo.…”

Section: Immunomodulatory Activity Of Pgpiv -1-amentioning

confidence: 99%

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Structural characterization and immunomodulatory activity of a new polysaccharide isolated from the radix of Platycodon grandiflorum

Shan

Xiong

Liu

et al. 2020

Int J of Food Sci Tech

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“…The phagocytic ability of RAW264.7 macrophages after treating with EGCG was determined by the neutral red assay, according to the previous method. , Briefly, the cells seeded in 96-well plates (1 × 10 5 cells/mL) were stimulated into the M1 phenotype and treated with different concentrations of EGCG (1, 5, 10, 25 μM) for 24 h simultaneously. The control group was set up as the cells without treating with EGCG.…”

Section: Methodsmentioning

confidence: 99%

Promotion Effect of EGCG on the Raised Expression of IL-23 through the Signaling of STAT3-BATF2-c-JUN/ATF2

Wang

et al. 2021

J. Agric. Food Chem.

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Tea polyphenol of epigallocatechin-3-gallate (EGCG) has been verified to possess multiple biological activities. is a heterodimeric cytokine consisting of two subunits of IL-23p19 and IL-12p40, with the functionality in regulating the production of cytokines under physiological or pathological conditions. By serendipity, the raised expression of IL-23 was observed after treating cells with EGCG, whereas the detailed mechanism remains poorly understood. This study was proposed to investigate the signaling related to EGCG-induced IL-23. The raised expression of IL-23 was confirmed primarily by intraperitoneally injecting with different concentrations of EGCG (0, 20, 50, 80 mg/kg) into BALB/c mice, and the raised expression was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Results from enzymelinked immunosorbent assay (ELISA) revealed the increase of IL-23 in serum from 116.09 to 153.90 pg/mL after treating with EGCG. The same results were also observed in RAW264.7 and peritoneal macrophages after treating with EGCG (0, 1, 5, 10, 25 μM) with the increased tendency of IL-23 in cultural medium (7.98 to 25.38 pg/mL for RAW264.7; 3.64 to 260.93 pg/mL for peritoneal macrophages). After preliminary exploration of the signaling related to the increased IL-23, the classical signaling pathways and key transcription factors, such as nuclear factor kappa-B (NF-κB), mitogen-activated protein kinase (MAPK) signaling pathways, and interferon regulatory factor 5 (IRF5), were demonstrated with no relevant contribution. A further study revealed the involvement of the key transcription factor of BATF2, which could antagonistically modulate the transcription and translation of IL-23. The signaling of STAT3-BATF2-c-JUN/ATF2-IL-23 has been further verified in RAW264.7 macrophages using the STAT3 inhibitor of AG490 and the activator of Colivelin TFA. The results indicated that EGCG inhibits the phosphorylation of STAT3 to facilitate the decreased level of BATF2, which contributed to the increased level of IL-23 by the enhancing heterodimerization of c-JUN and ATF2.

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A heteropolysaccharide purified from leaves of Ilex latifolia displaying immunomodulatory activity in vitro and in vivo

Cited by 28 publications

References 46 publications

The anti-obesity effects of a water-soluble glucan from Grifola frondosa via the modulation of chronic inflammation

The anti-obesity effects of a water-soluble glucan from Grifola frondosa via the modulation of chronic inflammation

Structural characterization and immunomodulatory activity of a new polysaccharide isolated from the radix of Platycodon grandiflorum

Promotion Effect of EGCG on the Raised Expression of IL-23 through the Signaling of STAT3-BATF2-c-JUN/ATF2

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