A hermaphrodite genotype in dioecious papaya progeny: sex reversal or contamination?

Pirovani, Adriana Azevedo Vimercati; Ramos, Helaine Christine Cancela; Santa-Catarina, Renato; Vettorazzi, Júlio Cesar Fiorio; Cortes, Diego Fernando Marmolejo; Boechat, Marcela Santana Bastos; Bohry, Dieimes; Pereira, Messias Gonzaga

doi:10.1007/s10681-018-2304-2

Cited by 7 publications

(5 citation statements)

References 31 publications

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“…This marker was developed from MSY regions, allowing for amplification in male and hermaphrodite plants, but not in female plants. The lack of bands in knowingly hermaphrodite plants has already been shown in other studies (Deputy et al 2002;Oliveira et al 2007;Pirovani et al 2018), but because the purity of DNA from endosperm is lower than that of DNA from leaves, the proportion of falsenegatives obtained in the present study was even more significant.…”

Section: Discussionsupporting

confidence: 70%

Molecular sexing in papaya (Carica papaya L.) seeds based on endosperm DNA

et al. 2020

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Section: Discussionsupporting

confidence: 70%

Molecular sexing in papaya (Carica papaya L.) seeds based on endosperm DNA

et al. 2020

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“…In papaya crop, this class of molecular marker has been used in phylogeographic and genetic structuring studies (Hasibuzzamanet al, 2020), analysis of genetic diversity and molecular characterization (Oliveira et al, 2010;Ramos et al, 2014;Pirovani et al, 2018), construction of a genetic map (Chen et al, 2007), identification of QTL (Blas et al, 2012), selection assisted by markers for the development of pure lines (Oliveira et al, 2012), among others.…”

Section: Introductionmentioning

confidence: 99%

MOLECULAR CHARACTERIZATION OF ELITE LINES OF PAPAYA (Carica papaya L.) VIA SSR MARKERS

Pirovani¹,

Ramos²,

Duarte³

et al. 2021

FPBJ

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This study aimed to analyze the genetic variability and to estimate diversity parameters of 23 elite papaya lines based on microsatellite molecular markers. The plant material was composed of elite lines, 18 from the 'Formosa' and five from the 'Solo' group. The expected heterozygosity (HE), observed heterozygosity (HO), and the coefficient of inbreeding (ƒ) for each genotype were estimated. The weighted index obtained the dissimilarity matrix, and the estimated genetic distance was presented graphically through the cluster analysis by the Ward method, as well as by the principal coordinates analysis (PCoA). The genotypes were also analyzed for genetic structure, using the Bayesian clustering method. Genetic variability was observed among the analyzed genotypes, mainly among the lines from the Formosa group. As for HO, four elite lines from the 'Solo' group had values equal to zero. However, the elite lines from the 'Formosa' group showed higher segregation in the loci with values ranging from 0.05 to 0.14. About ƒ, from the five lines in the 'Solo' group, four exhibited maximum fixation indexes for the analyzed loci, with the variation observed from 0.90 to 1.0, while the lines from the 'Formosa' group had a variation from 0.61 to 1.00. These results indicate the need for greater care in the process of obtaining inbreeding seeds to avoid pollen contamination, as well as the need to advance generations of self-fertilization with the lines from the 'Formosa' group to increase the level of inbreeding and ensure greater stability to hybrids that are developed from them.

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“…Likewise, the W11 and CpTrnL markers continue amplifying in male samples (Figure 3b) as well as in hermaphrodite-to-male sex reversal samples. Some authors reported good efficiency in a similar male-specific marker (PMSM1) in male-to-hermaphrodite sex reversal events (Liao et al 2017, Pirovani et al 2018, confirming their male specificity. Moreover, this marker serves dioecious varieties of papaya, leaving aside that papaya fruit production is mainly based on gynodioecious cultivars (Burns et al 2022).…”

Section: Jg áVila-hernández Et Almentioning

confidence: 80%

“…'Maradol' and, although faint amplification bands were observed, false negative results were not found (Figure 3a). Likewise, authors also reported false negative results (Deputy et al 2002, Oliveira et al 2007, Pirovani et al 2018, Duarte et al 2020. However, other markers related to hermaphrodite identification have been proposed yielding similar or better performance than W11 (Deputy et al 2002, Urasaki et al 2002, Chaves-Bedoya and Nuñez 2007, Urasaki et al 2012.…”

Section: Jg áVila-hernández Et Almentioning

confidence: 99%

Sex determination of papaya var. ‘Maradol’ reveals hermaphrodite-to-male sex reversal under greenhouse conditions

Ávila-Hernández,

Camas-Reyes,

Martinez-Antonio

2023

Crop Breed. Appl. Biotechnol.

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Papaya is a nutritious fruit cultivated worldwide under suitable climate conditions. This plant is polygamous, bearing female, male, and hermaphrodite sex types determined by sex chromosomes XX, XY, and XY h , respectively. In this paper, a molecular sex determination of papaya var. 'Maradol' was carried out based on PCR and specific primers. Specific molecular markers resulted in sixty-nine hermaphrodites and twenty-one female plants, matching 100% to the flower morphology. Nevertheless, since the summer stressing conditions rose in the greenhouse, sex reversal to male phenotype was observed in 43% of hermaphrodite plants due to high-temperature conditions. A specific male marker could not detect that change, supporting the proposal that sex reversal is caused by harsh environmental conditions aimed at epigenetic modification and genes related to hormones. Our study demonstrates the effectiveness of molecular sex determination and the importance of controlling the growing requirements of papaya to avoid sex reversal.

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A hermaphrodite genotype in dioecious papaya progeny: sex reversal or contamination?

Cited by 7 publications

References 31 publications

Molecular sexing in papaya (Carica papaya L.) seeds based on endosperm DNA

Molecular sexing in papaya (Carica papaya L.) seeds based on endosperm DNA

MOLECULAR CHARACTERIZATION OF ELITE LINES OF PAPAYA (Carica papaya L.) VIA SSR MARKERS

Sex determination of papaya var. ‘Maradol’ reveals hermaphrodite-to-male sex reversal under greenhouse conditions

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