A hemagglutinin with mitogenic activity from dark red kidney beans

Xia, Lixin; Ng, T.B.

doi:10.1016/j.jchromb.2006.07.042

Cited by 25 publications

(15 citation statements)

References 24 publications

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“…who recorded the lectin content in Phaseolus vulgaris seeds (red kidney beans) as 12 mg/g. On the other hand, our results disagreed with those of Ye et al [8] and Xia and Ng [25] who reported lower values of lectins from red and dark kidney beans (0.275 and 1.07 mg/g, respectively).…”

Section: Discussioncontrasting

confidence: 99%

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

El-S¹,

El-A²,

S³

2017

J Microb Biochem Technol

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Section: Discussioncontrasting

confidence: 99%

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

El-S¹,

El-A²,

S³

2017

J Microb Biochem Technol

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“…102,103 Liener (1991) 104 first time reported that lectin shows antitumor activity is the soybean agglutinin which capable to inhibit the growth of a transplanted tumor in rats. Xia and Ng 105 have reported the dark red kidney bean hemagglutinin exerted an antiproliferative activity toward leukemia L1210 cells. There are some other investigation shows that lectin has excellent antitumor activity that includes Banana lectin retarded proliferation of (L1210) cells and hepatoma (HepG2) cells.…”

Section: Antitumor Activitymentioning

confidence: 99%

Plant as a plenteous reserve of lectin

Ingale

Hivrale

2013

Plant Signaling & Behavior

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Lectins are clusters of glycoproteins of nonimmune foundation that combine specifically and reversibly to carbohydrates, mainly the sugar moiety of glycoconjugates, resulting in cell agglutination and precipitation of glycoconjugates. They are universally distributed in nature, being established in plants, fungi, viruses, bacteria, crustacea, insects, and animals, but leguminacae plants are rich source of lectins. The present review reveals the structure, biological properties, and application of plant lectins.

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“…The two black soybean lectins were adsorbed on SP Sepharose and Q Sepharose. Their other characteristics, including carbohydrate specificity and antiproliferative activity toward tumor cells, may not be all alike [24][25][26]30]. A 29-fold purification was obtained in the present study for small glossy black soybean lectin compared to 10-fold purification in the case of French bean lectin [28].…”

Section: Fig 6 Mitogenic Response Of Small Glossy Black Soybean Lectmentioning

confidence: 98%

“…French bean lectin was isolated using a similar procedure involving chromatography on SP Sepharose, Affi-gel blue gel, Q Sepharose and Superdex 200 [28]. This finding is reminiscent of the observation that lectins from different cultivars of the bean Phaseolus vulgaris, such as the pinto bean [24], haricot bean [25], red kidney bean [30], and dark red kidney bean [26], have similar but not identical N-terminal sequences. Thus, the three leguminous lectins appear to have similar chromatographic behavior.…”

Section: Fig 6 Mitogenic Response Of Small Glossy Black Soybean Lectmentioning

confidence: 99%

Purification of melibiose-binding lectins from two cultivars of Chinese black soybeans

Lin

2008

ABBS

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A dimeric 50 kDa melibiose-binding lectin was isolated from the seeds of the cultivar of soybean (Glycine max), called the small glossy black soybean. The isolation procedure comprised ion exchange chromatography on Q Sepharose, SP Sepharose and Mono Q followed by gel filtration on Superdex 75. The lectin was adsorbed on all three ion exchangers, and it exhibited an N-terminal sequence identical to that of soybean lectin. Of all the sugars tested, melibiose most potently inhibited the hemagglutinating activity of the lectin, which was stable between pH 3-12 and 0-70 degrees C. The lectin evoked maximal mitogenic response at about the same molar concentration as Con A. However, the response was much weaker. The soybean lectin inhibited the activity of HIV-1 reverse transcriptase as well as the proliferation of breast cancer MCF7 cells and hepatoma HepG2 cells with an IC50 of 2.82 microM, 2.6 microM and 4.1 microM, respectively. There was no antifungal activity. Another lectin was isolated from a different cultivar of soybean called little black soybean. The lectin was essentially similar to small glossy black soybean lectin except for a larger subunit molecular mass (31 kDa), a more potent mitogenic activity and lower thermostability. The results indicate that different cultivars of soybean produce lectins that are not identical in every aspect.

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A hemagglutinin with mitogenic activity from dark red kidney beans

Cited by 25 publications

References 24 publications

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

Plant as a plenteous reserve of lectin

Purification of melibiose-binding lectins from two cultivars of Chinese black soybeans

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