1943
DOI: 10.1097/00010694-194308000-00010
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A Handbook of Plant Tissue Culture

Abstract: Pflanzen aus Zellen, deren selbstandiges Leben unmittelbar nachweisen lasst.Da nun alle Zellen nach denselben Gesetzen wachsen, also nicht in einem Falle der Grund des Wachstum in der Zelle selbst, im andern Falle im ganzen Organismus liegen kann, da sich ferner nachweisen lasst, dass einzelne, von den iibrigen in der Art des Wachstums nicht verschiedene Zellen selbstandig sich entwickeln, so miissen wir iiberhaupt den Zellen ein selbstandiges Leben zuschreiben. . . . Dass nicht wirklich jede einzelne Zelle, w… Show more

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Cited by 142 publications
(122 citation statements)
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“…The axes from dry seed or seed that had been on the germination paper for up to 1.5 d were divided by length with one-quarter the length from the apex desgnated as the radicle, the following one-quarter length as the hypocotyl, and the remaining part as the hypocotyl hook. The separation of the hypocotyl hook from the hypocotyl is based on the observation that the hypocotyl hook portion turns greenish in color within hours after exposure to light ofdetached embryonic axes cultured in a nutrient agar medium (20). The hook portion in darkgerminated seedlings is yellowish in color.…”
Section: Materiais and Methods Plant Material Seeds Of The Soybean (mentioning
confidence: 99%
“…The axes from dry seed or seed that had been on the germination paper for up to 1.5 d were divided by length with one-quarter the length from the apex desgnated as the radicle, the following one-quarter length as the hypocotyl, and the remaining part as the hypocotyl hook. The separation of the hypocotyl hook from the hypocotyl is based on the observation that the hypocotyl hook portion turns greenish in color within hours after exposure to light ofdetached embryonic axes cultured in a nutrient agar medium (20). The hook portion in darkgerminated seedlings is yellowish in color.…”
Section: Materiais and Methods Plant Material Seeds Of The Soybean (mentioning
confidence: 99%
“…Other mineral salt solutions (White 1943;Linsmaer and Skoog 1965;medium N of Vogel 1956medium N of Vogel , 1964, supplemented with vitamins-glycine solution, trace elements, iron chelate, sucrose [see Section II(c) as for DBM1], and 2,4-dichlorophenoxyacetic acid (2 mg/I), failed to initiate and support callus growth from A. thaliana stems. Therefore the supplemented MS1 and MS2 (in the form of DBM1 and DBM2) were used as the basis for haploid tissue culture.…”
Section: (A) Preliminary Studiesmentioning
confidence: 99%
“…An attempt to tissue culture the makapuno embryo was first undertaken more than five decades ago [58], and was achieved two years later when De Guzman and Del Rosario [24] made use of a basal medium formulated by White [59] and Nitsch [60]. Coconut water, a good source of energy, with added vitamins and mineral nutrients, was also used at 25% (v/v) in this study, to enhance the germination rate.…”
Section: Embryo Culturementioning
confidence: 99%
“…Even though many well-known basal media, including White [59], Nitsch [60] and Murashige and Skoog [67] have been tried, the Y3 medium [68] is the most beneficial, being now most commonly used for makapuno embryo culture [62,66]. This medium provides a greater quantity of micro-elements for tissue growth, including cobalt, copper and particularly iodine.…”
Section: Embryo Culturementioning
confidence: 99%