Haploid Arabidopsis Thaliana Callus and Plants From Anther Culture

Gresshoff, P.M.; Doy, CH

doi:10.1071/bi9720259

Cited by 88 publications

(24 citation statements)

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“…The derivation and culture of haploid cell lines of Lycopersicon esculentum (tomato) ANU-H27-1 and Arabidopsis thaliana ANU-H872-1 have been described (2,3). L. esculentum ANU-H27-1 was maintained on DBM3 medium (2),t containing, in addition, napthylene acetic acid 8 Characterization of the T Gene Mutation.…”

Section: Methodsmentioning

confidence: 99%

“…The calluses described so far were maintained on media that do not lead to differentiation. Development and differentiation of the haploid cell lines of L. esculentum and A. thaliana can be controlled by variation of the components of the defined media (2,3). Cultures of A. thaliana greening on differentiation medium (1,3) are completely resistant to 480supF+.…”

Section: Transgenosis Of Gene Z (Lac+)mentioning

confidence: 99%

“…Preliminary reports (3,6) and a more detailed account of evidence and a discussion of background and results (1) have been presented.…”

mentioning

confidence: 99%

“…The recipient cells for transgenosis were mostly haploid cell lines of Lycopersicon esculentum and Arabidopsis thaliana recently developed in this laboratory (2,3). Haploidy is not essential for the present experiments, but has important implications for the future development of transgenosis (1).…”

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confidence: 99%

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Biological and Molecular Evidence for the Transgenosis of Genes from Bacteria to Plant Cells

Doy

Gresshoff

Rolfe

1973

Proc. Natl. Acad. Sci. U.S.A.

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Specialized transducing phages (X and 480) have been used as vectors in the transfer of genes We have tested for the possibility that bacterial genes can be transferred to, and then expressed within, plant cells. The experiments make use of the ability of specialized transducing phage (X, 480) to incorporate selected bacterial genes into their genome. These vector phage are simply added to plant cells in culture on fully defined media.For phenotypic expression, a sequence of gene transfer, maintenance, transcription, translation, and metabolic function must occur, except when the product of gene expression can function without translation (for example, a species of transfer RNA). After the initial transfer, the enzymic and structural elements for gene expression must be supplied by the plant cells. The overall phenomenon of transfer and subsequent expression has been termed transgenosis (1) to avoid a premature assumption of mechanisms inherent in the use of established terms, such as transduction, taken from bacterial genetics. Transgenosis is regarded as a particularly appropriate term when donor and recipient cells are separated widely by evolution and the mechanisms of gene transfer and maintenance (inheritance?) are obscure.The recipient cells for transgenosis were mostly haploid cell lines of Lycopersicon esculentum and Arabidopsis thaliana recently developed in this laboratory (2, 3). Haploidy is not essential for the present experiments, but has important implications for the future development of transgenosis (1).The biological detection of an advantageous transgenosis depended on survival and growth of plant cells on medium containing galactose and lactose as carbon sources. These environments are normally lethal (1, 4). Information for the metabolism of the sugars to usable sources of carbon was provided by the genes of the Escherichia coli galactose (gal) and lactose (lac) operons.A system where transgenosis might be a disadvantage was provided by "infection" with the E. coli mutant supF+ gene (also known as SulII +), which codes for a transfer RNA able to correct a nonsense mutation carried as a nonsense (amber) triplet in messenger RNA. Suppressor mutations lead to more mistakes in reading the genetic code and can be considered as unlikely to accumulate in higher organisms [see Watson (5) ]. If nonsense codons are vital to the life of plant cells, efficient suppression resulting from transgenosis of supF+ could be predicted to result in severe inhibition or death on normally optimal growth medium.Preliminary reports (3, 6) and a more detailed account of evidence and a discussion of background and results (1) have been presented. MATERIALS AND METHODSThe derivation and culture of haploid cell lines of Lycopersicon esculentum (tomato) ANU-H27-1 and Arabidopsis thaliana ANU-H872-1 have been described (2, 3). L. esculentum ANU-H27-1 was maintained on DBM3 medium (2)

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Section: Methodsmentioning

confidence: 99%

Section: Transgenosis Of Gene Z (Lac+)mentioning

confidence: 99%

“…Preliminary reports (3,6) and a more detailed account of evidence and a discussion of background and results (1) have been presented.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Biological and Molecular Evidence for the Transgenosis of Genes from Bacteria to Plant Cells

Doy

Gresshoff

Rolfe

1973

Proc. Natl. Acad. Sci. U.S.A.

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“…From these calli, plants are regenerated through organogenesis or indirect embryogenesis (Seguí-Simarro and Nuez 2007). This method is mostly studied in tomato, where it was possible to obtain complete DH plants in some cases (Seguí-Simarro and Nuez 2005;Zagorska et al 2004), and it was reported possible in some other species such as Arabidopsis (Gresshoff and Doy 1972), Vitis vinifera (Gresshoff and Doy 1974) or Digitalis purpurea (Corduan and Spix 1975).…”

Section: Other Androgenic Methodsmentioning

confidence: 99%

Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant

Sendra¹

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Thesis layout and cover design: Alba Rivas Sendra and André Arôxa Watercolor drawings: Alba Rivas Sendra AcknowledgementsEn primer lugar, quiero dar las gracias especialmente a Jose María, que me ha guiado en este proceso desde el primer momento. Me ha impulsado a crecer y, cuando no era posible, me ha traído un banquito para que pudiera alcanzar siempre más alto. Con tu ayuda me he convertido en una investigadora y todo lo que he aprendido me acompañará para siempre. Aunque a veces ponernos de acuerdo ha sido un reto, eso representa la esencia misma de la investigación y la ciencia, y lo agradezco sinceramente. Los retos siempre nos inspiran, nos obligan a aprender, a mantenernos activos y a tener menos miedo de las soluciones creativas. Y de esto es mejor tener los bolsillos llenos.También quiero expresar mi sincero agradecimiento a Kim Boutilier. Ella me abrió sus puertas el primer día, y las mantiene abiertas desde entonces. No solo aprendí cosas nuevas de los mejores expertos, sino que la estancia en su laboratorio me permitió ver un mundo diferente, encontrar un segundo hogar, sembrar y recoger muchísimo amor expatriado increíblemente verdadero, y darme cuenta de que son las cosas más diminutas (como puede ser una pequeña bici rosa) las que llenan nuestras vidas de felicidad.Familia, amigos, amigos que se convierten en familia, todos habéis tenido un papel en la parte de mí que queda reflejada en este libro. Los que siempre habéis caminado a mi lado, los que aparecisteis un día y ya nunca os marchasteis, los que acabáis de llegar pero es como si siempre hubieseis estado aquí, los que me dais la bienvenida cuando vuelvo a casa, donde quiera que mi casa esté, e incluso aquellos que solo me acompañaron durante una etapa... Todos vosotros habéis ayudado a dar forma a la persona que soy hoy. No hay un momento del que me arrepienta, un beso que desee nunca haber dado, una palabra que preferiría no haber oído. Ha sido un largo camino, pero esto es solo el comienzo. Las olas siguen llegando a la playa, las células siguen creciendo en nuestras placas de cultivo, y la vida, como el espectáculo, debe continuar. Lo único importante es ser feliz. Siempre hacia delante y apuntando alto, retroceder nunca, rendirse jamás (Corey Yuen, 1986).First of all, I specially want to thank Jose María, who has guided me through this life-changing process since the very beginning. He encouraged me to grow, and when it was not possible, he brought me a stool so I could always reach higher.With your help I became a researcher, and everything I learnt will be with me forever. Even though sometimes it was challenging to come to an agreement, this represents the core essence of research and science, and I truly appreciate it.Challenges always inspire us, make us learn, keep us active and take away our fear of creative solutions. And it is always better to have plenty of these.I also want to express my sincere thanks to Kim Boutilier. She opened her door to me on the first day, and still holds it open. Not only have I learnt new things from...

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