A Greener and Faster Approach for Determination of Phenolic Compounds by Smartphone-Based Colorimetry

Abstract

This work aims the development of an analytical alternative to determine phenolic compounds in fruits exploiting a greener microwave-assisted extraction and photometric detection by smartphone-based colorimetry. Acerola cherry (Malpighia emarginata) was selected as a model and the extraction was optimized by the Doehlert design, taking into account temperature, solvent composition, and extraction time. The Folin–Ciocalteau method was used to determine total phenolic content in the extracts, with a 5-fold reduction in reagent amount and waste generation. The optimal extraction was achieved with 17% v/v ethanol, at 38 °C, for 50 min. The total phenolic content determined by the proposed procedure (146 ± 4 mg GAE g−1 dry weight) agreed with the reference procedure (145.0 ± 0.7 mg GAE g−1 dry weight) at the 95% of confidence level. Due to these characteristics, the proposed approach is an alternative for screening fruits in research with bioactive compounds.

Metodologias analíticas alternativas para a avaliação de autenticidade de alimentos explorando análises hiperespectrais e fotometria por imagens digitais

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