A green homogeneous liquid-liquid microextraction method for spectrophotometric determination of daclatasvir in human plasma

Abdallah, Inas A.; Hammad, Sherin F.; Bedair, Alaa; Mansour, Fotouh R.

doi:10.1016/j.scp.2021.100498

Cited by 10 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Homogenous liquid-liquid microextraction (HLLME) is a novel sample preparation technique that has attracted much attention from many researchers [13,[25][26][27][28]. It is based on the phase separation of a homogeneous mixture and the simultaneous extraction of the target analytes into one of the separated phases.…”

Section: Introductionmentioning

confidence: 99%

Salting‐out homogeneous liquid‐liquid microextraction for the determination of azole drugs in human urine: Validation using total error concept

Manousi

Vlachaki

Kika

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

A salting-out homogeneous liquid-liquid microextraction was proposed for the quantification of four azole drugs in human urine prior to high-performance liquid chromatography analysis. The procedure involved the mixing of the sample with acetonitrile in appropriate volumes followed by the addition of sodium sulfate solution in order to facilitate phase separation. The parameters influencing the extraction performance were studied and optimized using a two-step experimental design. The analytical procedure was thoroughly validated using the accuracy profiles as a graphical decision-making tool.The β-expectation tolerance intervals did not exceed the acceptance criteria of ±15% meaning that 95% of future results will be included in the defined bias limits. The limits of detection of the procedure were satisfactory, ranging between 0.01 and 0.03 μg/mL. The mean analytical bias in the spiking levels was satisfactory and ranged between -10.3 and 4.2% while the relative standard deviation was lower than 5.6%. Monte-Carlo simulations followed by capability analysis were employed to investigate the ruggedness of the sample preparation protocol. The developed method offers advantages compared to previously reported approaches for the same type of analysis including extraction efficiency and scaling down of the sample volume and extraction time.

show abstract

Section: Introductionmentioning

confidence: 99%

Salting‐out homogeneous liquid‐liquid microextraction for the determination of azole drugs in human urine: Validation using total error concept

Manousi

Vlachaki

Kika

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…In HLLE, the organic solvent is mixed with the aqueous sample to form a homogenous phase, before being separated by addition of salt or sugar. The hydrophilic nature of the extractants in HLLE allows extraction of highly polar analytes [15] , [16] . Hydrophobic solvent-assisted HLLE (HSLLE) is as a special mode of HLLE in which a hydrophobic solvent (such as chloroform [17] , dichloromethane [18] or toluene [19] ) is used to induce phase separation instead of salts and sugars.…”

Section: Introductionmentioning

confidence: 99%

Menthol-assisted homogenous liquid-liquid microextraction for HPLC/UV determination of favipiravir as an antiviral for COVID-19 in human plasma

Abdallah

Hammad

Bedair

et al. 2022

Journal of Chromatography B

Self Cite

View full text Add to dashboard Cite

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Hammad

Abdallah

Bedair

et al. 2021

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Liquid-liquid extraction is a widely used technique of sample preparation in biomedical analysis. In spite of the high pre-concentration capacities of liquidliquid extraction, it suffers from a number of limitations including time and effort consumption, large organic solvent utilization, and poor performance in highly polar analytes. Homogeneous liquid-liquid extraction is an alternative sample preparation technique that overcomes some drawbacks of conventional liquid-liquid extraction, and allows employing greener organic solvents in sample treatment. In homogeneous liquid-liquid extraction, a homogeneous phase is formed between the aqueous sample and the water-miscible extractant, followed by chemically or physically induced phase separation. To form the homogeneous phase, aqueous samples are mixed with water-miscible organic solvents, waterimmiscible solvents/cosolvents, surfactants, or smart polymers. Then, phase separation is induced chemically (adding salt, sugar, or buffer) or physically (changing temperature or pH). This mode is rapid, sustainable, and cost-effective in comparison with other sample preparation techniques. Moreover, homogeneous liquid-liquid extraction is more suitable for the extraction of delicate macromolecules such as enzymes, hormones, and proteins and it is more compatible with liquid chromatography with tandem mass spectrometry, which is a vital technique in metabolomics and proteomics. In this review, the principle, types, applications, automation, and technical aspects of homogeneous liquid-liquid extraction are discussed.

show abstract

A green homogeneous liquid-liquid microextraction method for spectrophotometric determination of daclatasvir in human plasma

Cited by 10 publications

References 31 publications

Salting‐out homogeneous liquid‐liquid microextraction for the determination of azole drugs in human urine: Validation using total error concept

Salting‐out homogeneous liquid‐liquid microextraction for the determination of azole drugs in human urine: Validation using total error concept

Menthol-assisted homogenous liquid-liquid microextraction for HPLC/UV determination of favipiravir as an antiviral for COVID-19 in human plasma

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Contact Info

Product

Resources

About