A green chemistry approach towards synthesizing hydrogel for sustained ocular delivery of brinzolamide: In vitro and ex vivo evaluation

Chakole, Chandrashekhar Mahadeo; Sahoo, PK; Pandey, Jaya; Chauhan, Meenakshi Kanwar

doi:10.1016/j.jics.2021.100323

Cited by 10 publications

(7 citation statements)

References 36 publications

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“…The assay measures changes in cell membrane integrity as a result of cell death. By binding DNA in cells that have lost their membrane integrity (dead cells), the fluorescence property of the assay dye is greatly enhanced [36][37][38][39][40][41][42]. Pure brinzolamide suspension was used as a standard for comparison (Figure 8).…”

Section: Cytotoxicity Studymentioning

confidence: 99%

Preparation and In Vitro Testing of Brinzolamide-Loaded Poly Lactic-Co-Glycolic Acid (PLGA) Nanoparticles for Sustained Drug Delivery

Ako-Adounvo,

Karla

2024

JCTO

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Glaucoma therapy aims at lowering intra-ocular pressure (IOP). Brinzolamide, a carbonic anhydrase inhibitor, is utilized as a second-line medication for treating ocular hypertension and primary open-angle glaucoma (POAG). The drug lowers the IOP making it a therapeutic agent against glaucoma, and due to its poor water solubility, is commercially available as Azopt®, a 1% ophthalmic suspension. Adverse effects such as blurred vision, ocular irritation, discomfort, and bitter taste are associated with the use of the marketed brinzolamide formulation. This study aims to test the feasibility of formulating and in vitro testing of brinzolamide-PLGA nanoparticles for improved toxicity profile. The nanoparticles were prepared by the oil-in-water (O/W) emulsion-solvent evaporation method. Particle size and zeta potential were determined by dynamic light scattering (DLS). The morphology of the nanoparticles was determined by scanning electron microscopy (SEM). Encapsulation of the drug was verified by high-performance liquid chromatography (HPLC) and the compatibility of the polymer and drug was verified by Fourier transform infrared (FTIR) spectroscopy. The in vitro drug release profile was assessed employing the dialysis method. Intracellular localization of the nanoparticles was assessed by confocal microscopy utilizing Rhodamine 123-loaded nanoparticles. Cytotoxicity of the formulation was assessed on Statens Seruminstitut Rabbit Cornea (SIRC) and transfected Human Corneal Epithelial (SV40 HCEC) cell lines. The particle size of the nanoparticle formulations ranged from 202.3 ± 2.9 nm to 483.1 ± 27.9 nm for blank nanoparticles, and 129.6 ± 1.5 nm to 350.9 ± 8.5 nm for drug-loaded nanoparticles. The polydispersity of the formulations ranged from 0.071 ± 0.032 to 0.247 ± 0.043 for blank nanoparticles, and 0.089 ± 0.028 to 0.158 ± 0.004 for drug-loaded nanoparticles. Drug loading and encapsulation efficiencies ranged from 7.42–15.84% and 38.93–74.18%, respectively. The in vitro drug release profile for the optimized formulation was biphasic, with a ~54% burst release for the initial 3 h, followed by a cumulative 85% and 99% released at 24 and 65 h, respectively. Uptake study showed nanoparticles(NPs) localization in the cytoplasm and around the nuclei of the cells. Brinzolamide-PLGA nanoparticles were successfully developed, characterized, and tested in vitro. Preliminary data show intracellular localization of the nanoparticles in the cytoplasm of SIRC and SV40 HCEC cells. The formulations appeared to be relatively non-cytotoxic to the cells. The research data from the study provided preliminary data for successful development and promising in vitro absorption efficacy for brinzolamide-loaded PLGA nanoparticle formulation.

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Section: Cytotoxicity Studymentioning

confidence: 99%

Preparation and In Vitro Testing of Brinzolamide-Loaded Poly Lactic-Co-Glycolic Acid (PLGA) Nanoparticles for Sustained Drug Delivery

Ako-Adounvo,

Karla

2024

JCTO

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“…Due to the various properties like high strength, heat resistance, electrical conductivity, structure, and electron affinity it has wide commercial applications [49]. Fullerenes are extensively studied nanoparticles because of its uses in medicinal field such as in binding particular antibodies to the system to point resistant bacteria.…”

Section: Fullerenesmentioning

confidence: 99%

“…4 . Due to the various properties like high strength, heat resistance, electrical conductivity, structure, and electron affinity it has wide commercial applications 49 .…”

Section: Types Of Nanostructures Used For Drug Deliverymentioning

confidence: 99%

Nanomedicines: A Focus on Nanomaterials as Drug Delivery System with Current Trends and Future Advancement

et al. 2022

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The rapid advancement of nanomedicine presents novel alternatives that have the potential to transform health care. Targeted drug delivery as well as the synthesis of nanocarriers is a growing discipline that has been intensively researched to reduce the complexity of present medicines in a variety of diseases and to develop new treatment and diagnostic techniques. There are several designed nanomaterials used as a delivery system such as liposomes, micelles, dendrimers, polymers, carbon-based materials, and many other substances, which deliver the drug moiety directly into its targeted body area reducing toxic effect of conventional drug delivery, thus reducing the amount of drug required for therapeutic efficacy and offering many more advantages. Currently, these are used in many applications, including cancer treatment, imaging contrast agents, and biomarker detection and so on. This review provides a comprehensive update in the field of targeted nano-based drug delivery systems, by conducting a thorough examination of the drug synthesis, types, targets, and application of nanomedicines in improving the therapeutic efficiency.

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“…(Dalaf et al, 2022). Green chemistry It is a technique in chemical preparations that aims to reduce the emitted substances resulting from emissions in chemical manufacturing to the least possible extent (Quispe et al, 2022), as it introduces new techniques that can facilitate human life and activities without harming the environment (Chakole et al, 2022), as green chemistry includes the use of modern techniques and chemical methods that reduce substances polluting the environment or prohibiting the use of raw materials (Gangrade et al, 2015), solvents or catalytic auxiliary agents and materials that may disintegrate and which are hazardous to the environment and humans (Liu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization of some new Heterocyclic Compounds Derived from Chalcones Containing Schiff Bases

Ghanim¹,

Omar²

2023

RJS

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The present work involves the new chalcones containing Schiff bases, In this study, firstly, reaction of 3-acetylcoumarin with terephthaldehyde to prepared chalcones which contains a free aldehyde group which has been reacted with (4-Aminoantipyrine) to prepare Schiff bases, which was reacted (sodiumazide) as precursor to form (Tetrazole) followed by condensation of these chalcones with some compounds (hydroxylamine, aminophenol, phenylenediamine, hydrazine, semicarbazide, thiosemicarbazide, urea, thiourea and quanidine) as precursor to form Isoxazole, oxazepane, diazepine, pyrazoles and pyrimidine derivatives in basic medium using classical and ultrasonic technique. The comparison of the classical methods with ultrasonic methods. Was achieved. The compositions of the prepared compounds were confirmed by FT-IR and 1 H NMR spectroscopy.

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A green chemistry approach towards synthesizing hydrogel for sustained ocular delivery of brinzolamide: In vitro and ex vivo evaluation

Cited by 10 publications

References 36 publications

Preparation and In Vitro Testing of Brinzolamide-Loaded Poly Lactic-Co-Glycolic Acid (PLGA) Nanoparticles for Sustained Drug Delivery

Preparation and In Vitro Testing of Brinzolamide-Loaded Poly Lactic-Co-Glycolic Acid (PLGA) Nanoparticles for Sustained Drug Delivery

Nanomedicines: A Focus on Nanomaterials as Drug Delivery System with Current Trends and Future Advancement

Synthesis, Characterization of some new Heterocyclic Compounds Derived from Chalcones Containing Schiff Bases

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