A gradient anion exchange chromatographic method for the speciation of arsenic in lobster tissue extracts

Brisbin, Judith A.

doi:10.1016/s0039-9140(02)00262-x

Cited by 54 publications

(22 citation statements)

References 38 publications

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“…20 -22 Cation exchange can be used to separate the cationic species AB, AC, TeMAs + and TMAO, 22 in which As III , As V , MMA and DMA are expected to co-elute together. 22 Alternatively, ion pairing reversed-phase HPLC can be used to resolve inorganic arsenic and organoarsenicals in a single chromatographic run with help of ion-pairing agents. Tetrabutyl ammonium hydroxide is a common ionpairing agent used for separating anionic species, 25,26 and pentanesulfonate 19,24 or hexanesulfonate 25,26 are used for separating cationic species.…”

Section: Speciation Analysis and Environmentmentioning

confidence: 99%

“…However, problems arise when other cationic species are present; for instance, if AC present, it usually co-elutes with AB, 18 -22 as does TeMAs + and TMAO. 20 -22 Cation exchange can be used to separate the cationic species AB, AC, TeMAs + and TMAO, 22 in which As III , As V , MMA and DMA are expected to co-elute together. 22 Alternatively, ion pairing reversed-phase HPLC can be used to resolve inorganic arsenic and organoarsenicals in a single chromatographic run with help of ion-pairing agents.…”

Section: Speciation Analysis and Environmentmentioning

confidence: 99%

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Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

Karthikeyan

Hirata

2004

Applied Organom Chemis

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Arsenic speciation analysis in marine samples was performed using ion chromatography (IC) with inductively coupled plasma mass spectrometry (ICP-MS) detection. The separation of eight arsenic species, viz. arsenite, monomethyl arsonic acid, dimethylarsinic acid, arsenate, arsenobetaine, tetramethylarsine oxide, arsenocholine and tetramethylarsonium ion was achieved on a Dionex AS4A (weaker anion exchange column) by using a nitric acid pH gradient eluent (pH 3.3 to 1.3). The entire separation was accomplished in 12 min. The detection limits for the eight arsenic species by IC-ICP-MS were in the range 0.03-1.6 µg l −1 , based on 3σ of the blank response (n = 6). The repeatability and day-to-day reproducibility were calculated to be less than 10% (residual standard deviation) for all eight species. The method was validated by analyzing a certified reference material (DORM-2, dogfish muscle) and then successfully applied to several marine samples, e.g. oyster, fish muscle, shrimp and marine algae. The low power microwave digestion was employed for the extraction of arsenic from seafood products.

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Section: Speciation Analysis and Environmentmentioning

confidence: 99%

Section: Speciation Analysis and Environmentmentioning

confidence: 99%

Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

Karthikeyan

Hirata

2004

Applied Organom Chemis

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“…Some online coupling systems of chromatographic separation with special element detection techniques are robust enough for As speciation analysis (Brisbin et al 2002;Day et al 2002). Among the reported methods, HPLC is the most commonly used separation technique because of its efficient, rapid and convenient separation and analysis procedure (Gong et al 2002).…”

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confidence: 99%

A comparative study of the extractability of arsenic species from silverbeet and amaranth vegetables

Rahman

Chen

Naidu

2009

Environ Geochem Health

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There is still no reliable standard extraction method for the speciation of arsenic (As) in plant tissue, and hence there is great interest in developing one for plants that are used as human food. Speciation and bioavailability are critical for accurate human health risk assessment, as As species vary in both their toxicity and bioavailability. Recent incidences of As poisoning in many countries have led to significant research into the fate and dynamics of As in the soil and water environment, including speciation. Although one of the major pathways of ingestion of As is via food, only limited research has been conducted to assess the nature and proportion of various As species present in food crops. In this study, we compared the efficacy of ammonium dihydrogen phosphate and protein extracting solution for the extractability of As from two different species of spinach (amaranth and silverbeet). We found that a microwave-assisted technique with protein extracting solution was most effective, yielding 76-114% extractability and excellent separation and speciation of all As species present in the spinach matrices. The stability test for extracted As species showed them as stable for 45 days without any significant loss or inter-conversion. Both As III and As V were identified in the shoots of amaranth and silverbeet. However, the percentage of As species varied between amaranth and silverbeet. The silverbeet shoot showed a somewhat higher percentage of As V , while the amaranth showed a higher percentage of As III . The samples contained mostly inorganic As, especially As III ([90%) in the edible part of the vegetables, a form that is more toxic and bioavailable than other organic and methylated species.

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“…Carbonate up to 70 mmol/L (pH 12) had to be applied on a AS9HC column to separate As-species within 5 minutes [40]. The advantage of a gradient (15-30 mmol/L) vs. isocratic (30 mmol/L) separation was demonstrated [154] using NH 4 HCO 3 pH = 8.5 on a PRP-X100 column. The gradient provided almost baseline separation of the cationic AC and the neutral AB.…”

Section: Selective Separation Methodsmentioning

confidence: 99%

Arsenic Speciation Analysis by Ion Chromatography - A Critical Review of Principles and Applications

Ammann¹

2011

AJAC

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Multiple acute and chronic toxicity of arsenic species and its mobilisation from geological deposits into ground and drinking water resources are one of the greatest threats to human health. Arsenic speciation analysis, mostly done by liquid chromatography, is a challenging task which requires an intense high quality work with respect to extraction, preservation, separation, detection and validation. A growing number of As-species and low regulatory limits (10 μg/L) may require more than one speciation method preferably performed by species specific procedures and detectors. Beside As-fractionation for special application there are many selective speciation methods based on high performance separation techniques like capillary electrophoreses, gas and liquid chromatography. Both, fractionation and speciation methods are reviewed. However, the focus is on scopes and limits of ion chromatographic separations, the most frequently used methods. Based on IC-principles the methods applied are critically discussed and recommendations given which should result in more robust and reliable As-speciation.

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A gradient anion exchange chromatographic method for the speciation of arsenic in lobster tissue extracts

Cited by 54 publications

References 38 publications

Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

A comparative study of the extractability of arsenic species from silverbeet and amaranth vegetables

Arsenic Speciation Analysis by Ion Chromatography - A Critical Review of Principles and Applications

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