A gold nanoparticles based immuno-bioprobe for detection of Vi capsular polysaccharide of Salmonella enterica serovar Typhi

Pandey, Satish Kumar; Suri, C. Raman; Chaudhry, Muhammad Tausif; Tiwari, Raj Gaurang; Rishi, Praveen

doi:10.1039/c2mb25048a

Cited by 25 publications

(11 citation statements)

References 31 publications

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“…50 Each of the tested conditions increases in the diameter size more than 30 nm, a result regularly seen during the formation of pAb–AuNP nanoprobes. 44,51 This is a primary disadvantage to this type of the bioconjugation method as crosslinking of the carbodiimide bridge can attribute to the increase in the particle size. 17 The DLS diameters by number match the ζ-potential changes (Figures 2 and S2, S3), with the highest Δζ-potential values corresponding to the largest size increase.…”

Section: Results and Discussionmentioning

confidence: 99%

Optimization and Structural Stability of Gold Nanoparticle–Antibody Bioconjugates

et al. 2019

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Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors exploiting unique surface chemistries and optical properties. Many efforts have focused on antibody bioconjugation to AuNPs resulting in a sensitive bioconjugate to detect specific types of bacteria. Unfortunately, bacteria thrive under various harsh environments, and an understanding of bioconjugate stability is needed. Here, we show a method for optimizing Listeria monocytogenes polyclonal antibodies bioconjugation mechanisms to AuNPs via covalent binding at different pH values, from 2 to 11, and 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid, NaOH, HCl conditions. By fitting Lorentz curves to the amide I and II regions, we analyze the stability of the antibody secondary structure. This shows an increase in the apparent breakdown of the antibody secondary structure during bioconjugation as pH decreases from 7.9 to 2. We find variable adsorption efficiency, measured as the percentage of antibody adsorbed to the AuNP surface, from 17 to 27% as pH increases from 2 to 6 before decreasing to 8 and 13% at pH 7.9 and 11, respectively. Transmission electron microscopy (TEM) analysis reveals discrepancies between size and morphological changes due to the corona layer assembly from antibody binding to single nanoparticles versus aggregation or cluster self-assembly into large aggregates. The corona layer formation size increases from 3.9 to 5.1 nm from pH 2 to 6, at pH 7.9, there is incomplete corona formation, whereas at pH 11, there is a corona layer formed of 6.4 nm. These results indicate that the covalent binding process was more efficient at lower pH values; however, aggregation and deactivation of the antibodies were observed. We demonstrate that optimum bioconjugation condition was determined at pH 6 and MES buffer-type by indicators of covalent bonding and stability of the antibody secondary structure using Fourier transform-infrared, the morphological characteristics and corona layer formation using TEM, and low wavelength shifts of ultraviolet–visible after bioconjugation.

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Section: Results and Discussionmentioning

confidence: 99%

Optimization and Structural Stability of Gold Nanoparticle–Antibody Bioconjugates

et al. 2019

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“…This model was applied for some other samples whose details have been reported in previous articles. 24,[45][46][47][48] In Table 2, the refractive index of the coating shell (n 2 ) is obtained for the samples. The near values of the refractive index of citrate ions (in cases 1, 2, 6, and 8) can be assigned to considerable validity of this method.…”

Section: Resultsmentioning

confidence: 99%

Effect of conjugation with organic molecules on the surface plasmon resonance of gold nanoparticles and application in optical biosensing

Koushki

2021

RSC Adv.

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“…In another effort, a sandwich immunoassay was developed using nitrocellulose membranes immobilized with anti-Salmonella Abs to bind with Salmonella typhi expressing Vi capsular polysaccharide vaccine (or ViCPS) using anti-Vi Ab-functionalized 30-nm AuNPs. This method can detect not only Vi, but also whole Salmonella bacteria (Pandey et al, 2012).…”

Section: Pathogensmentioning

confidence: 99%

LSPR‐based colorimetric biosensing for food quality and safety

Wang

You

Gunasekaran

2021

Comp Rev Food Sci Food Safe

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Ensuring consistently high quality and safety is paramount to food producers and consumers alike. Wet chemistry and microbiological methods provide accurate results, but those methods are not conducive to rapid, onsite testing needs. Hence, many efforts have focused on rapid testing for food quality and safety, the advances in nanotechnology, this sensing technique lends itself easily for further development on field-deployable platforms such as smartphones for onsite and end-user applications.

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A gold nanoparticles based immuno-bioprobe for detection of Vi capsular polysaccharide of Salmonella enterica serovar Typhi

Cited by 25 publications

References 31 publications

Optimization and Structural Stability of Gold Nanoparticle–Antibody Bioconjugates

Optimization and Structural Stability of Gold Nanoparticle–Antibody Bioconjugates

Effect of conjugation with organic molecules on the surface plasmon resonance of gold nanoparticles and application in optical biosensing

LSPR‐based colorimetric biosensing for food quality and safety

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