A Glycoprotein Surface Layer Covers the Pseudomurein Sacculus of the Extreme Thermophile Methanothermus fervidus

Nußer, Elisabeth; Hartmann, Erwin; Allmeier, Helmut; König, Helmut; Paul, Gisela; Stetter, Karl-Otto

doi:10.1007/978-3-642-73537-0_5

Cited by 10 publications

(4 citation statements)

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“…Some archaeal S‐layers have shown to be highly resistant to common denaturizing agents (Beveridge, ). Special isolation procedures are required for S‐layers in Archaea where they are associated with the cytoplasmic membrane (Nußer et al ., ; König et al ., ; Rachel, ). With many solubilized S‐layers, it has been demonstrated that isolated subunits reassemble into lattices identical to those observed on intact cells upon removal of the disrupting agent (see also section ‘’).…”

Section: Isolation and Chemistrymentioning

confidence: 98%

“…Generally, S‐layers are isolated from cell wall fragments which were obtained by breaking up the cells and removing the content including the cytoplasmic membrane by addition of hydrogen bond‐breaking agents [e.g. guanidine hydrochloride (GHCl) or urea] (Sleytr & Messner, ; Schuster et al ., ; Schuster & Sleytr, ), trichloroacetic acid (Nußer et al ., ), detergents, or cation substitution (e.g. Na + or Li + , replacing Ca 2+ ; Koval & Murray, ; Lortal et al ., , ), and ethylene diamine tetraacetic acid (EDTA; Cline et al ., ).…”

Section: Isolation and Chemistrymentioning

confidence: 99%

“…With the exception of selected methanogenic Archaea (e.g. M. fervidus ) where the S‐layer is located on the surface of a rigid pseudomurein sacculus (Nußer et al ., ; Fig. c) in most Archaea , S‐layers assemble as exclusive wall component in close association with the plasma membrane and consequently have been connected with a cell shape maintaining role (Mescher & Strominger, ; Messner et al ., ; Pum et al ., ; Claus & König, ; Albers & Meyer, ).…”

Section: Assembly and Morphogenesismentioning

confidence: 99%

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S-layers: principles and applications

et al. 2014

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Monomolecular arrays of protein or glycoprotein subunits forming surface layers (S-layers) are one of the most commonly observed prokaryotic cell envelope components. S-layers are generally the most abundantly expressed proteins, have been observed in species of nearly every taxonomical group of walled bacteria, and represent an almost universal feature of archaeal envelopes. The isoporous lattices completely covering the cell surface provide organisms with various selection advantages including functioning as protective coats, molecular sieves and ion traps, as structures involved in surface recognition and cell adhesion, and as antifouling layers. S-layers are also identified to contribute to virulence when present as a structural component of pathogens. In Archaea, most of which possess S-layers as exclusive wall component, they are involved in determining cell shape and cell division. Studies on structure, chemistry, genetics, assembly, function, and evolutionary relationship of S-layers revealed considerable application potential in (nano)biotechnology, biomimetics, biomedicine, and synthetic biology.

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Section: Isolation and Chemistrymentioning

confidence: 98%

Section: Isolation and Chemistrymentioning

confidence: 99%

Section: Assembly and Morphogenesismentioning

confidence: 99%

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S-layers: principles and applications

et al. 2014

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“…However, a few other examples are also known (9,25). Surface layers appear to be more frequently glycosylated in archaebacteria (12,19,21,27) than in eubacteria (25). Most data available on eubacterial glycoproteins originate from S-layers of different strains of the family Bacillaceae (25).…”

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confidence: 99%