Crystalline Bacterial Cell Surface Layers (S-Layers) as Combined Carrier/Adjuvants for Conjugate Vaccines

Malcolm, Andrew J.; Messner, Paul; Sleytr, Uwe B.; Smith, Richard H.; Unger, Frank M.

doi:10.1007/978-1-4471-3479-4_13

Cited by 17 publications

(6 citation statements)

References 25 publications

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“…Because a reproducible immobilization of peptide epitopes to common carriers which were used as monomers in solution or as dispersions of unstructured aggregates on aluminum salts could not be achieved (Brown et al ., ; Powell & Newman, ), the use of regularly structured S‐layer self‐assembly products as immobilization matrices was envisaged. Therefore, several conjugate vaccines with S‐layer (glyco)proteins and carbohydrate antigens (poly‐ and oligosaccharides), haptens or the recombinant birch pollen allergen Bet v1 were produced which showed promising results in vaccination trials (Sleytr et al ., ; Messner et al ., ; Malcolm et al ., , b; Smith et al ., ; Jahn‐Schmid et al ., , b; Messner et al ., ; Jahn‐Schmid et al ., ).…”

Section: Applicationsmentioning

confidence: 99%

S-layers: principles and applications

et al. 2014

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Monomolecular arrays of protein or glycoprotein subunits forming surface layers (S-layers) are one of the most commonly observed prokaryotic cell envelope components. S-layers are generally the most abundantly expressed proteins, have been observed in species of nearly every taxonomical group of walled bacteria, and represent an almost universal feature of archaeal envelopes. The isoporous lattices completely covering the cell surface provide organisms with various selection advantages including functioning as protective coats, molecular sieves and ion traps, as structures involved in surface recognition and cell adhesion, and as antifouling layers. S-layers are also identified to contribute to virulence when present as a structural component of pathogens. In Archaea, most of which possess S-layers as exclusive wall component, they are involved in determining cell shape and cell division. Studies on structure, chemistry, genetics, assembly, function, and evolutionary relationship of S-layers revealed considerable application potential in (nano)biotechnology, biomimetics, biomedicine, and synthetic biology.

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Section: Applicationsmentioning

confidence: 99%

S-layers: principles and applications

et al. 2014

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“…Secondary and tertiary immunization could be performed using the same hapten(s) coupled to different immunologically non-cross-reactive S-layers, thus preventing the tolerance frequently observed with toxoid carriers. The data gathered also showed that S-layers vaccines have an intrinsic adjuvant property Malcolm et al 1993).…”

Section: Conjugated Vaccinesmentioning

confidence: 88%

“…An even broader application potential for S-layers can be seen in their use as immobilization matrix and adjuvant for weakly immunogenic antigens and haptens Messner et al 1992a;Malcolm et al 1993). Both glycosylated and non-glycosylated S-layers have been used as vaccine carriers.…”

Section: Conjugated Vaccinesmentioning

confidence: 99%

“…The observation that prokaryotes carrying S-layers are ubiquitously found in every part of the biosphere supports the concept that these supramolecular structures can fulfil a broad spectrum of functions. Based on the data obtained by fundamental studies on Slayers, a considerable potential for the crystalline arrays in biotechnological, biomedical and non-biological applications including areas such as molecular nanotechnology became evident (for reviews see Pum et al 1992see Pum et al , 1993Sleytr et al 1992;Malcolm et al 1993;Sara et al 1993).…”

Section: Introductionmentioning

confidence: 99%

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Crystalline bacterial cell surface layers: general principles and application potential

Sleytr

Messner

Pum

et al. 1993

Journal of Applied Bacteriology

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“…S-layer proteins have been crystallized and cross-linked on the surface of liposomes to stabilize the lipid membrane by modulating the lateral diffusion of the free lipid molecules, thus changing their fluidity and increasing their mechanical stability (Paul et al, 1992). And the suitability of S-layers as carrier/adjuvants for the production of conjugate vaccines has been described by Malcolm et al (1993).…”

Section: Immobilization Matrix/carriersmentioning

confidence: 99%

Two-dimensional (2D) crystallization of engineered proteins: A review

Lü¹,

Shen²,

Li³

et al. 2012

Afr. J. Microbiol. Res.

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Protein two-dimensional (2D) crystallization denotes the supreme degree of its ordering, thus 2D protein crystals can be applied in diverse fields ranging from structural biology to materials science with unique performances. To date, 2D crystallization of proteins, especially engineered proteins, is emerging as a powerful tool for bottom-up approaches to the nanofabrication of functional structures. However, till now there is still lack of general method of forming 2D crystals of proteins due to limited understanding of the 2D crystallization process, and the self-assembly of 2D protein crystals often suffers from point defects and grain boundaries resulting from homogeneous nucleation and uncontrollable crystal growth. In this review, we will introduce some aspects of 2D protein crystallization, including key approaches to growing 2D protein crystals and their potential applications in various fields.

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Crystalline Bacterial Cell Surface Layers (S-Layers) as Combined Carrier/Adjuvants for Conjugate Vaccines

Cited by 17 publications

References 25 publications

S-layers: principles and applications

S-layers: principles and applications

Crystalline bacterial cell surface layers: general principles and application potential

Two-dimensional (2D) crystallization of engineered proteins: A review

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