A glucuronoxylomannan-like glycan produced by Trichosporon mucoides

Zimbres, Ana Claudia G.; Albuquerque, Priscila Costa; Joffe, Luna S.; Souza, Taiane N.; Nimrichter, Leonardo; Frazão, Stefânia O.; Albuquerque, Patrícia; Fonseca, Fernanda L.; Rodrigues, Márcio L.

doi:10.1016/j.fgb.2018.09.009

Cited by 7 publications

(20 citation statements)

References 38 publications

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“…MAb 18B7 labeling ranged from a dotted to a ring pattern, confirming previous indications by our group (Cordero et al, 2016). This same binding pattern was originally reported for Cn (Casadevall et al, 1998) and other GXM-like polysaccharide producing fungi, such as C. liquefaciens (Araujo et al, 2017), T. asahii (Fonseca et al, 2009), T. mucoides (Zimbres et al, 2018) and P. brasiliensis (Albuquerque et al, 2012).…”

Section: Discussionsupporting

confidence: 89%

“…Regardless of the relative composition of the GXM-like polysaccharides and the serological reactivity to Cn GMX mAbs, C-gly-Hc and E-gly-Hc were effectively incorporated onto the surface of Cn cap59 acapsular mutants impaired phagocytosis by macrophages and enhancing yeast intracellular survival in these phagocytes. These results provide additional evidence for the similarity among the GXM-like polysaccharides and further support their protective activity to other fungi against phagocytes, suggesting that they might be involved in fungal pathogenesis in distinct models (Fonseca et al, 2009;Albuquerque et al, 2012;Araujo et al, 2017;Zimbres et al, 2018).…”

Section: Discussionsupporting

confidence: 68%

“…As a GXM-like component, C-gly-Hc could be promptly incorporated by the Cn cap59 acapsular mutant, forming a capsule-like structure, with some dotted regions, resembling the capsule formed when C-gly-Cn was used. A similar profile was also observed for the incorporation of other GXM-like components of the most diverse origin, likely indicating a shared property among them (Fonseca et al, 2009;Albuquerque et al, 2012;Araujo et al, 2017;Zimbres et al, 2018). As for Cn GXM, C-gly-Hc incorporation could occur via a-1,3-glucan (Reese and Doering, 2003) or attachment to cell wall chitin (Ramos et al, 2012).…”

Section: Discussionsupporting

confidence: 62%

“…T. asahii GXM-like, in turn, displays a relatively higher number of mannosyl units and distinct positions of xylosyl substitutions per motif (Fonseca et al, 2009). The closely related T. mucoides displays a very similar composition to T. asahii, except for the higher number of glucuronic acid substitutions (Zimbres et al, 2018). The P. brasiliensis GXM-like polysaccharide is mainly composed of mannose and galactose, and traces of glucose, xylose, and rhamnose, with an absence of glucuronic acid (Albuquerque et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Trichosporon asahii produces a functional GXM-like molecule with similar glycosyl composition to cryptococcal GXM that also manifests antiphagocytic activities (Fonseca et al, 2009). Recently, Zimbres et al also described the production of GXM-like polysaccharides by T. mucoides (Zimbres et al, 2018). In the phylum Ascomycota, GXM-like structures have been identified in the dimorphic fungus Paracoccidioides brasiliensis (Albuquerque et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan

Gonçalves

Noval

Ferreira

et al. 2021

Front. Cell. Infect. Microbiol.

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The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species. Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties. The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM. Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo. In this work, we characterized the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs. These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition. Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database. Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions. These C-gly-Hc and E-gly-Hc also reacted with sera of cryptococcosis patients. In turn, sera from histoplasmosis patients recognized Cn glycans, suggesting immunogenicity and the presence of cross-reacting antibodies. Additionally, C-gly-Hc and E-gly-Hc coated Cn cap59 were more resistant to phagocytosis and macrophage killing. C-gly-Hc and E-gly-Hc coated Cn cap59 were also able to kill larvae of Galleria mellonella. These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.

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