Production of Secreted Carbohydrates that Present Immunologic Similarities with the Cryptococcus Glucuronoxylomannan by Members of the Trichosporonaceae Family: A Comparative Study Among Species of Clinical Interest

Andrade, Iara Bastos de; Almeida, Marcos de Abreu; Figueiredo-Carvalho, Maria Helena Galdino; Coelho, Rowena Alves; Chaves, Alessandra Leal da Silva; Frasés, Susana; Zancopé‐Oliveira, Rosely Maria; Brito‐Santos, Fabio; Almeida‐Paes, Rodrigo

doi:10.1007/s11046-021-00558-w

Cited by 4 publications

(5 citation statements)

References 34 publications

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“…epidermidis in the presence of commercial polysaccharides dextran (α1,6-glucan) and laminarin (β1,3-glucan) and PIS that were extracted from S. epidermidis by using the ethanol precipitation method. − Figure a shows that the presence of all three polysaccharides at a concentration of 4.5 μg/mL can significantly reduce the antibacterial activity of G3 and increase the MIC by more than 2 times. Compared with dextran and laminarin, PIS had the most obvious effects and could increase the MIC by 3 times.…”

Section: Resultsmentioning

confidence: 99%

Antibiofilm Mechanisms of the Helical G3 Peptide against Staphylococcus epidermidis

Chen,

Gu,

et al. 2024

Langmuir

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Antibacterial peptides (ABPs) have been recognized as promising alternatives to conventional antibiotics due to their broad antibacterial spectrum, high antibacterial activity, and low possibility of inducing bacterial resistance. However, their antibiofilm mechanisms have not yet reached a consensus. In this study, we investigated the antibiofilm activity of a short helical peptide G3 against Staphylococcus epidermidis, one of the most important strains of medical device contamination. Studies show that G3 inhibits S. epidermidis biofilm formation in a variety of ways. In the initial adhesion stage, G3 changes the properties of bacterial surfaces, such as charges, hydrophobicity, and permeability, by rapidly binding to them, thus interfering with their initial adhesion. In the mature stage, G3 prefers to target extracellular polysaccharides, leading to the death of outside bacteria and the disruption of the three-dimensional (3D) architecture of the bacterial biofilm. Such efficient antibiofilm activity of G3 endows it with great potential in the treatment of infections induced by the S. epidermidis biofilm.

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Section: Resultsmentioning

confidence: 99%

Antibiofilm Mechanisms of the Helical G3 Peptide against Staphylococcus epidermidis

Chen,

Gu,

et al. 2024

Langmuir

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“…reported that Trichosporon asahii Y2 could increase lipase activity in the digestive tract and promote obesity and hyperlipidemia. Andrade et al [ 23 ]. reported that T. asahii and T. mucoides could produce glucuronoxylomannan, which participates in several immunoregulatory mechanisms and causes inflammation.…”

Section: Discussionmentioning

confidence: 99%

Alteration of Colonic Bacterial and Fungal Composition and Their Inter- and Intra-Kingdom Interaction in Patients with Adenomas with Low-Grade Dysplasia

et al. 2023

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Colorectal cancer (CRC) develops from pre-cancerous cellular lesions in the gut epithelium and mainly originates from specific types of colonic adenomas with dysplasia. However, gut microbiota signatures among sampling sites in patients with colorectal adenomas with low-grade dysplasia (ALGD) and normal control (NC) remain uncharacterized. To characterize gut microbial and fungal profiles in ALGD and normal colorectal mucosa tissues. We used 16S and ITS1-2 rRNA gene sequencing and bioinformatics analysis on the microbiota of ALGD and normal colorectal mucosa from 40 subjects. Bacterial sequences in the ALGD group showed an increase in Rhodobacterales, Thermales, Thermaceae, Rhodobacteraceae, and several genera, including Thermus, Paracoccus, Sphingobium, and Pseudomonas, compared to the NC group. Fungal sequences in the ALGD group showed an increase in Helotiales, Leotiomycetes, and Basidiomycota, while several orders, families, and genera, including Verrucariales, Russulales, and Trichosporonales, were decreased. The study found various interactions between intestinal bacteria and fungi. The bacterial functional analysis showed increased glycogen and vanillin degradation pathways in the ALGD group. Meanwhile, the fungal functional analysis showed a decrease in pathways related to the biosynthesis of gondoate and stearate, as well as degradation of glucose, starch, glycogen, sucrose, L-tryptophan, and pantothenate, and an increase in the octane oxidation pathway in the ALGD group. The mucosal microbiota in ALGD exhibits altered fungal and microbial composition compared to the NC mucosa, potentially contributing to the development of intestinal cancer by regulating specific metabolic pathways. Therefore, these changes in microbiota and metabolic pathways may be potential markers for diagnosing and treating colorectal adenoma and carcinoma.

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“…The sensitivity with the serum samples is 100% and the specificity is 99%. Only the genus Trichosporon , which also produces glucoronoxylomannan-like molecules [ 141 ], caused a false positive reaction with this test [ 142 ].…”

Section: Cryptococcosismentioning

confidence: 99%

Immunologic Diagnosis of Endemic Mycoses

Almeida‐Paes

Bernardes-Engemann

Motta

et al. 2022

JoF

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The endemic mycoses blastomycosis, coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, cryptococcosis, sporotrichosis, talaromycosis, adiaspiromycosis, and emergomycosis are mostly caused by geographically limited thermally dimorphic fungi (except for cryptococcosis), and their diagnoses can be challenging. Usual laboratory methods involved in endemic mycoses diagnosis include microscopic examination and culture of biological samples; however, serologic, histopathologic, and molecular techniques have been implemented in the last few years for the diagnosis of these mycoses since the recovery and identification of their etiologic agents is time-consuming and lacks in sensitivity. In this review, we focus on the immunologic diagnostic methods related to antibody and antigen detection since their evidence is presumptive diagnosis, and in some mycoses, such as cryptococcosis, it is definitive diagnosis.

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Production of Secreted Carbohydrates that Present Immunologic Similarities with the Cryptococcus Glucuronoxylomannan by Members of the Trichosporonaceae Family: A Comparative Study Among Species of Clinical Interest

Cited by 4 publications

References 34 publications

Antibiofilm Mechanisms of the Helical G3 Peptide against Staphylococcus epidermidis

Antibiofilm Mechanisms of the Helical G3 Peptide against Staphylococcus epidermidis

Alteration of Colonic Bacterial and Fungal Composition and Their Inter- and Intra-Kingdom Interaction in Patients with Adenomas with Low-Grade Dysplasia

Immunologic Diagnosis of Endemic Mycoses

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