A glucose biosensor based on the synergistic action of nanometer-sized TiO2 and polyaniline

Tang, Wenwei; Li, Lei; Zeng, Xin

doi:10.1016/j.talanta.2014.08.019

Cited by 97 publications

(35 citation statements)

References 20 publications

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“…The relatively low detection limit, wide detection range and the facile and rapid determination procedure make this detection system promising for direct, practical glucose determination in biological samples. Quenching of gold nanoparticles 0.0-11.11 mM - [52] pH induced on formational switch of i-motif DNA 5.0-100.0 µM 4.0 µM [53] Fe 3 O 4 peroxidase mimetic strategy 0.05-10.0 µM 0.025 µM [54] Nanometer-sized TiO 2 and polyaniline 0.02-6.0 mM 18.0 µM [55] Gold nanoclusters-based photoelectrochemical sensors 75.0-408.0 µM - [56] CdS quantum dots-based glucose sensing 0.1 µM-1.0 mM 20.0 nM [57] Anionic dye and a cationic quencher/receptor 1.0-20.0 mM 1.0 mM [58] Porphyrin-modified NiO nanoparticles 0.05-5.0 mM 0.02 mM [59] 3-Aminophenylboronicacid-functionalized CuInS 2 QDs 0.005-8.0 mM 1.2 µM [60] Polyethyleneimine-templated Cu nanoclusters 10.0-100.0 µM 8.0 µM [61] This method 25.0 µM-75.0 mM 0.5 µM -…”

Section: Resultsmentioning

confidence: 99%

A nanosensor for determination of glucose based on silver nanoparticles as fluorescence probes

et al. 2015

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afforded by nanoscale technologies are expected to have substantial impacts on almost all industries and areas of society (e.g., medicine, plastics, energy, electronics, and aerospace) [1][2][3][4][5]. The recent advancement of bioanalytical techniques involving the development of highly sensitive or selective analytical methods based on nanoscale materials/molecules have progressed with remarkable success. In this regard, silver nanoparticles (AgNPs) have received significant attention in constructing analytical and bioanalytical sensors. This relevance arises from their unusual optical, electronic, and chemical properties [6][7][8]. They have a high surface area, very small size (<20 nm) and high dispersion ability, spectrally selective coating [9, 10], surface-enhanced Raman scattering [11,12], and antibacterial activity [13]. Moreover, AgNPs exhibit optical properties which could not be observed either in molecular or in bulk metallic form [14][15][16][17].Fluorescence spectroscopy as a sensitive, simple, diverse, and nondestructive method can provide real time, in situ, and dynamic analytical information [18]. However, utilizing nanoparticles in fluorescence analysis has provided significant improvement in this area [19][20][21][22][23]. It should be mentioned that the fluorescence of metal clusters and thin films is well established based on Mooradian's observation of photoluminescence from bulk copper and gold [24]. Also several reports are available related to the fluorescence of silver nanoclusters [25,26].Glucose, a simple sugar (monosaccharide), is an important carbohydrate in biology. Cells use it as a source of energy and a metabolic intermediate and its lack or excess can produce detrimental influence on cellular functions. The glucose level in blood is considered as a clinical indicator for diabetes. Therefore, the monitoring of glucose level in blood with faster and more accurate methods is in great demand and a great deal of effort has been focused Abstract A nanosensor was designed for the enzymatic determination of glucose based on the fluorescence enhancement of silver nanoparticles (AgNPs). This enhancement is the result of the glucose oxidase-catalyzed oxidation of glucose. Silver nanoparticles were prepared through a chemical reduction by using trisodium citrate as the reducing agent and were characterized with UV-visible spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscopy. The experimental results showed that the increase of the AgNP fluorescence was linearly proportional to the concentration of glucose within its concentration ranges of 2.50 × 10 −5 -7.50 × 10 −3 M and 7.50 × 10 −3 -7.50 × 10 −2 M with a detection limit of 5.53 × 10 −7 M under the optimized experimental conditions. To evaluate the applicability of the nanobiosensor, determination of glucose in real samples was performed according to the developed procedure.

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Section: Resultsmentioning

confidence: 99%

A nanosensor for determination of glucose based on silver nanoparticles as fluorescence probes

et al. 2015

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“…Therefore, chitosan can be used as a gel matrix for enzyme immobilization through GAL or another reagent . Several electropolymers were used to fabricate the glucose biosensor such as polypyrrole (PPy) (Ozyilmaz et al 2011, Raicopol et al 2013, Olea et al 2008, polyaniline (PANI) (Ozdemir et al 2010, Yao et al 2015, Tang et al 2015, poly(o-anisidine) (POA) (Savale & Shirsat 2009, Borole et al 2007), poly(o-phenylenediamine) (Rothwell et al 2010), polythophen derivative (Abasiyanik et al 2010). …”

Section: Introductionmentioning

confidence: 99%

Poly(N-Methylpyrrole)-Chitosan layers for Glucose Oxidase Immobilization for Amperometric Glucose Biosensor Design

Özyılmaz¹,

Özyılmaz²,

Akyürekoğlu³

2017

Natural and Engineering Sciences

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In this study, Pt electrode was coated by poly(N-methypyrrol) (PNMP) film, then Glucose Oxidase (GOD) was immobilized onto PNMP layer with thin chitosan (Chi) gel and finally, electrode was reacted with glutaraldehyde (GAL) to form crosslinking between -NH2 groups of Chi and GOD to prevent enzyme leakage from Chi. GOD-based electrode was used to measure current response depending on glucose concentration by chronoamperometric method. Due to preparation of electrode conditions have significant effect on current values which were measured and optimized in presence of glucose, polymer synthesis and GOD immobilization conditions detailed. Therefore, the effect of N-methylpyrrole monomer concentration, scan rate, Chi concentration, GOD concentration and GAL concentration on biosensor response was investigated by classical method. In sight of obtained data, optimal monomer concentration and scan rates for PNMP synthesis were determined as 50 mM and 20 mV/s, respectively. Optimal Chi, GOD and GAL concentrations were found as 1,00%, 4 mg/mL and 0.025 %, respectively. SEM images of Pt, PNMP coated Pt and GOD immobilized Pt electrodes were obtained; Imax and KM values were calculated using Lineweaver-Burk plot. After 20 successive uses of same enzyme electrode in 5 mM glucose solution, it kept still its 91.3 % of initial activity.

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“…To improve the performance of electrochemical non-enzymatic glucose sensor, the above nanomaterials have been functionalized by conductive polymer in general, such as polyaniline (PANi), polypyrrole, polythiophene, and etc [25,26] . PANi has been used as a typical conducting polymer in glucose biosensor [27] . Owing to conjugation in PANi molecule, electrons possess a high degree of transferability [28] .…”

Section: Introductionmentioning

confidence: 99%

One-step electrochemical fabrication of a nickel oxide nanoparticle/polyaniline nanowire/graphene oxide hybrid on a glassy carbon electrode for use as a non-enzymatic glucose biosensor

et al. 2016

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We propose here a novel non-enzymatic glucose biosensor based on nickel oxide nanoparticles/polyaniline 10 nanowire/graphene oxide modified glassy carbon electrode (NiONPs/PANiNW/GO/GCE). The composite, prepared by mixing aniline with graphene oxide (GO) together, was transferred to the surface of bare glassy carbon electrode (GCE). The modified electrode was then immersed into deoxygenated 50 mM NiCl2 solution and electrodeposited at -0.8V for 400s to obtain NiONPs/PANiNW/GO/GCE. We characterized the morphology and electrochemical performance of the modified electrode using scanning electron microscopy (SEM) and cyclic voltammetry (CV), respectively. We found that 15NiONPs/PANiNW/GO/GCE exhibited higher electrocatalytic activity to glucose oxidation than nickel oxide nanosheets/graphene oxide modified glassy carbon electrode (NiONS/GO/GCE) in alkaline solution. The sensitivity of the sensor towards glucose oxidation was 376.22 μA mM −1 cm −2 with a linearity range of 2 μM -5.560 mM and a detection limit of 0.5 μM (S/N = 3). The sensor selectively detected glucose in presence of the common interfering species such as ascorbic acid, uric acid, and dopamine. Furthermore, we examined the applicability of this modified electrode as a sensing 20 probe for the detection of the glucose concentrations in the fetal bovine serum. We conclude that the highly selective and sensitive NiONPs/PANiNW/GO/GCE based non-enzymatic glucose sensor has potential to be applied for accurate measurement of glucose level for various practical purposes such as clinical diagnosis, food analysis, etc.

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A glucose biosensor based on the synergistic action of nanometer-sized TiO2 and polyaniline

Cited by 97 publications

References 20 publications

A nanosensor for determination of glucose based on silver nanoparticles as fluorescence probes

A nanosensor for determination of glucose based on silver nanoparticles as fluorescence probes

Poly(N-Methylpyrrole)-Chitosan layers for Glucose Oxidase Immobilization for Amperometric Glucose Biosensor Design

One-step electrochemical fabrication of a nickel oxide nanoparticle/polyaniline nanowire/graphene oxide hybrid on a glassy carbon electrode for use as a non-enzymatic glucose biosensor

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