A Global eDNA Comparison of Freshwater Bacterioplankton Assemblages Focusing on Large-River Floodplain Lakes of Brazil

Abstract

With its network of lotic and lentic habitats that shift during changes in seasonal connection, the tropical and subtropical large-river systems represent possibly the most dynamic of all aquatic environments. Pelagic water samples were collected from Brazilian floodplain lakes (total n = 58) in four flood-pulsed systems (Amazon [n = 21], Araguaia [n = 14], Paraná [n = 15], and Pantanal [n = 8]) in 2011–2012 and sequenced via 454 for bacterial environmental DNA using 16S amplicons; additional abiotic field and laboratory measurements were collected for the assayed lakes. We report here a global comparison of the bacterioplankton makeup of freshwater systems, focusing on a comparison of Brazilian lakes with similar freshwater systems across the globe. The results indicate a surprising similarity at higher taxonomic levels of the bacterioplankton in Brazilian freshwater with global sites. However, substantial novel diversity at the family level was also observed for the Brazilian freshwater systems. Brazilian freshwater bacterioplankton richness was relatively average globally. Ordination results indicate that Brazilian bacterioplankton composition is unique from other areas of the globe. Using Brazil-only ordinations, floodplain system differentiation most strongly correlated with dissolved oxygen, pH, and phosphate. Our data on Brazilian freshwater systems in combination with analysis of a collection of freshwater environmental samples from across the globe offers the first regional picture of bacterioplankton diversity in these important freshwater systems.Electronic supplementary materialThe online version of this article (doi:10.1007/s00248-016-0834-5) contains supplementary material, which is available to authorized users.

Metagenomic profiling of the microbial freshwater communities in two Bulgarian reservoirs

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“…In general, BCC is shaped by a combination of different mechanisms, which might vary with the environmental characteristics of each system (Szekely & Langenheder, 2014). However, few studies have addressed these questions in tropical river-floodplain systems (but see Lemke et al, 2009;Tessler et al, 2017) where high temporal variability and spatial heterogeneity add additional layers of complexity.…”

“…In the Amazon basin, most studies have investigated only the spatial dynamics of BCC (Tessler et al, 2017) or were restricted to sampling either the river's main channel and tributaries or the plume/ estuary (Ghai et al, 2011;Satinsky et al, 2015). In contrast, few studies have considered both spatial and seasonal scales (Doherty et al, 2017).…”

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