Blood Transfusion Unit (BTU) is a blood bank of Indonesian Red Cross (IRC) which duties are to give service, collect blood donation, store and distribute blood according to the request of people in need. Administration process of BTU Merauke is in trouble due to conventional blood donation data management. Therefore, it difficult to specify the number of blood bag in BTU. Also, people who need blood donation must come to BTU office. E-Blood Bank Application is an information system which used to organize blood donation data and order blood online. The application is developed electronically so that it can provide information on blood stock in real time and connect it to BTU, people who need blood donors, and the donor community. Hence, blood stock information can be accessed through easily accessed media at any time and anywhere as long as it is connected to the network. Based on the result of implementation and testing application, it can be concluded that organizing blood donor data electronically through application may present real time blood stock, donor history and order blood bag online. Thus, it helps the service of BTU to people.