A genome-wide association study to identify candidate genes for erectile dysfunction

Kazemi, Elham; Zargooshi, Javaad; Kaboudi, Marzieh; Heidari, Parviz; Kahrizi, Danial; Mahaki, Behzad; Mohammadian, Youkhabeh; Khazaei, Habibolah; Ahmed, Kawsar

doi:10.1093/bib/bbaa338

Cited by 76 publications

(73 citation statements)

References 87 publications

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“…According to recent reviews, cell-based and cell-free therapies (exosomes, extracellular vesicles, microRNAs) have been successfully applied to angiogenesis-mediated tissue regeneration with great potential in the treatment of ischemic heart disease, stroke and bone defect [16] . In addition, exosomatic derived communication between endothelial cells and cardiomyocytes shows the potential to induce local neovascularization during heart injury [17,18] . In this study, we found that the protein expressions of angiogenic factors VEGFA and VEGFR2 decreased in MCAO rats, which was related to the decrease of angiogenesis and the regulation of VEGFA and VEGFR2 was released.…”

Section: Resultsmentioning

confidence: 99%

Molecular Mechanism of Bone Marrow Mesenchymal Stem Cell Derived Exosomes on Vascular Repair in Rats with Brain Injury

Ma¹,

Cao²,

Sun³

et al. 2021

ijps

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To explore the molecular mechanism of exosomes derived from bone marrow mesenchymal stem cells on vascular repair in rats with brain injury. 30 Sprague-Dawley rats were used in this study. Bone marrow was extracted from the posterior iliac crest of the rat under general anesthesia and bone marrow mesenchymal stem cells were isolated and cultured. Some rats were treated with middle cerebral artery occlusion surgery to induce acute brain injury models. The protein expressions of vascular endothelial growth factor A and vascular endothelial growth factor receptor 2 were analyzed by western blot. Polymerase chain reaction was used to analyze the messenger RNA expressions of endothelin-1, vascular cell adhesion molecule 1 and endothelial nitric oxide synthase related to endothelial cell damage. The protein expressions of vascular endothelial growth factor A and vascular endothelial growth factor receptor 2 in the model group was lower than that in the control group (p<0.05) and the protein expressions in the treatment group was higher than that in the model group (p<0.05). The messenger RNA expressions of endothelin-1 and vascular cell adhesion molecule 1 in the model group increased while the expression of endothelial nitric oxide synthase messenger RNA decreased (p<0.05). The messenger RNA expressions of endothelin-1 and vascular cell adhesion molecule 1 in the treatment group decreased and the messenger RNA expression of endothelial nitric oxide synthase increased (p<0.05). The apoptosis level of endothelial cells in the model group increased and in the treatment group was reduced. Exosomes derived from bone marrow mesenchymal stem cells could effectively regulate cell survival, promote angiogenesis, protect nerve cells and improve the prognosis of stroke in rats by up regulating the expression of C-X-C chemokine receptor type 4 in bone marrow mesenchymal stem cells.

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Section: Resultsmentioning

confidence: 99%

Molecular Mechanism of Bone Marrow Mesenchymal Stem Cell Derived Exosomes on Vascular Repair in Rats with Brain Injury

Ma¹,

Cao²,

Sun³

et al. 2021

ijps

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show abstract

“…Protein-protein interactions can significantly modulate various cellular functions, such as replication, transcriptional adjustment, growth and development, signaling processes, and coordination of multiple metabolic systems [50][51][52]. The role of StSOT proteins in biosynthetic processes of secondary metabolites indicates their critical functions during proper potato growth and tuberization and stress responses through signaling pathways [50,51].…”

Section: Discussionmentioning

confidence: 99%

Investigation and Computational Analysis of the Sulfotransferase (SOT) Gene Family in Potato (Solanum tuberosum): Insights into Sulfur Adjustment for Proper Development and Stimuli Responses

Faraji

Heidari

Amouei

et al. 2021

Plants

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Various kinds of primary metabolisms in plants are modulated through sulfate metabolism, and sulfotransferases (SOTs), which are engaged in sulfur metabolism, catalyze sulfonation reactions. In this study, a genome-wide approach was utilized for the recognition and characterization of SOT family genes in the significant nutritional crop potato (Solanum tuberosum L.). Twenty-nine putative StSOT genes were identified in the potato genome and were mapped onto the nine S. tuberosum chromosomes. The protein motifs structure revealed two highly conserved 5′-phosphosulfate-binding (5′ PSB) regions and a 3′-phosphate-binding (3′ PB) motif that are essential for sulfotransferase activities. The protein–protein interaction networks also revealed an interesting interaction between SOTs and other proteins, such as PRTase, APS-kinase, protein phosphatase, and APRs, involved in sulfur compound biosynthesis and the regulation of flavonoid and brassinosteroid metabolic processes. This suggests the importance of sulfotransferases for proper potato growth and development and stress responses. Notably, homology modeling of StSOT proteins and docking analysis of their ligand-binding sites revealed the presence of proline, glycine, serine, and lysine in their active sites. An expression essay of StSOT genes via potato RNA-Seq data suggested engagement of these gene family members in plants’ growth and extension and responses to various hormones and biotic or abiotic stimuli. Our predictions may be informative for the functional characterization of the SOT genes in potato and other nutritional crops.

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“…Also, the fertility rate in the group that had a positive culture with pathological bacteria was significantly lower than the group with a negative culture. Embryo transfer can overcome many of the problems of sexual dysfunction that result in unorganized marriages (19)(20)(21).…”

Section: Caspase 3 Expressionmentioning

confidence: 99%

Comparison of pregnancy rates following fresh and frozen embryo transfer in women undergoing assisted reproductive techniques

Haje

Shabila

2022

Cell Mol Biol (Noisy-le-grand)

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Freezing embryos is the best way to increase fertility for women with ovarian hyper-stimulation syndrome and women at risk for ovarian dysfunction. Due to the importance of freezing and pregnancy embryos, the present study was conducted to compare the fertility rate and the affecting factors following fresh embryos and frozen embryos in women treated with assisted reproductive techniques. In this study, 250 infertile women and IVF/ICSI candidates were studied. Embryos were used in fresh or frozen groups for transfer to the uterus. The expression of the caspase-3 gene was also examined for further evaluation. Data analysis was performed using SPSS 16 software, Chi-square, independent t-test, and Kruskal-Wallis tests. Out of 250 infertile women, 96 (38.4%) became pregnant, of which 54 were in the fresh embryo group and 42 were in the frozen embryo group, which was not statistically significant (P=0.32). Infertility causes, number of embryonic cells and grading of transferred embryos, delivery complications, embryo implantation methods, number of produced embryos after delivery, and endometrial thickness were not significantly different between the two groups (P=0.53). The difference between the mean number of transferred embryos in the two groups was significant (P<0.05), which was no longer significant after excluding non-pregnant women, and in comparing with only pregnant women (P=0.36). The result of caspase-3 gene expression showed that there was significant differences between fresh embryos, healthy thawed frozen embryos, and destroyed thawed frozen embryos. But these results were totally different from the results of the Pregnancy rate section. Therefore, it is inferred that although caspase-3 genes are expressed in frozen embryos after thawing and are ready to destroy the embryo, there are probably a number of involved factors that prevent the activity of caspase-3 and do not allow the apoptotic process to occur. What these factors are and how they prevent this process needs further study.

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A genome-wide association study to identify candidate genes for erectile dysfunction

Cited by 76 publications

References 87 publications

Molecular Mechanism of Bone Marrow Mesenchymal Stem Cell Derived Exosomes on Vascular Repair in Rats with Brain Injury

Molecular Mechanism of Bone Marrow Mesenchymal Stem Cell Derived Exosomes on Vascular Repair in Rats with Brain Injury

Investigation and Computational Analysis of the Sulfotransferase (SOT) Gene Family in Potato (Solanum tuberosum): Insights into Sulfur Adjustment for Proper Development and Stimuli Responses

Comparison of pregnancy rates following fresh and frozen embryo transfer in women undergoing assisted reproductive techniques

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