A genome-wide analysis of wide compatibility in rice and the precise location of the S5 locus in the molecular map

Liu, K. D.; Wang, J.; Li, H. B.; Xu, Caiguo; Liu, A. M.; Li, X. H.; Zhang, Q.

doi:10.1007/s001220050629

Cited by 107 publications

(54 citation statements)

References 8 publications

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“…Through RFLP analysis, three loci (one major and two minor) conferring significant effects on fertility restoration have been identified 23 . The segregation pattern of crosses showing trigenic epistatic interaction has also been reported by many workers 8,[11][12][13] .…”

Section: Resultsmentioning

confidence: 99%

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Hossain¹,

Singh²,

Fasih-uz-Zaman³

2010

ScienceAsia

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Exploitation of the higher degree of heterosis manifested in inter sub-specific (indica and indica/japonica) derivatives is one of the current trends in hybrid rice breeding. The success in developing indica/japonica hybrids using new plant type restorers developed from indica/japonica derivatives largely depends on the availability of effective restorers and knowledge of the genetics of fertility restoration of such derivative lines. A study using three indica

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Section: Resultsmentioning

confidence: 99%

Untitled

Hossain¹,

Singh²,

Fasih-uz-Zaman³

2010

ScienceAsia

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“…For example, hybrid sterility between different varieties of the cultivated rice, O. sativa, is often genetically simple (Oka 1974;Liu et al 1997;Yoshimura 2002, 2005, but also see . In this system, Kubo and Yoshimura (2002) have genetically mapped both partners of a two-locus incompatibility with major effects on hybrid viability and fertility.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the genetic complexity that typifies Drosophila hybrid male sterility is not necessarily mirrored in other systems. Remarkably, hybrid sterility between varieties of cultivated rice, Oryza sativa, is often genetically simple (Oka 1974;Liu et al 1997;Kubo and Yoshimura 2002;Kubo and Yoshimura 2005). It also is not clear that hybrid male sterility should always evolve more readily than female sterility or lethality.…”

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confidence: 99%

A Simple Genetic Incompatibility Causes Hybrid Male Sterility in Mimulus

2006

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Much evidence has shown that postzygotic reproductive isolation (hybrid inviability or sterility) evolves by the accumulation of interlocus incompatibilities between diverging populations. Although in theory only a single pair of incompatible loci is needed to isolate species, empirical work in Drosophila has revealed that hybrid fertility problems often are highly polygenic and complex. In this article we investigate the genetic basis of hybrid sterility between two closely related species of monkeyflower, Mimulus guttatus and M. nasutus. In striking contrast to Drosophila systems, we demonstrate that nearly complete hybrid male sterility in Mimulus results from a simple genetic incompatibility between a single pair of heterospecific loci. We have genetically mapped this sterility effect: the M. guttatus allele at the hybrid male sterility 1 (hms1) locus acts dominantly in combination with recessive M. nasutus alleles at the hybrid male sterility 2 (hms2) locus to cause nearly complete hybrid male sterility. In a preliminary screen to find additional small-effect male sterility factors, we identified one additional locus that also contributes to some of the variation in hybrid male fertility. Interestingly, hms1 and hms2 also cause a significant reduction in hybrid female fertility, suggesting that sex-specific hybrid defects might share a common genetic basis. This possibility is supported by our discovery that recombination is reduced dramatically in a cross involving a parent with the hms1-hms2 incompatibility.

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“…The experimental procedures for Southern blot analysis, including DNA extraction, enzyme digestion, electrophoresis, and hybridization, were essentially as described previously (37). The PCR product of GUS (β-glucuronidase) gene using GUS1.6F and GUS1.6R as primers was purified and prepared as probe for Southern blot analysis.…”

Section: Methodsmentioning

confidence: 99%

Linking differential domain functions of the GS3 protein to natural variation of grain size in rice

Mao¹,

Sun²,

Yao

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

610

511

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Grain yield in many cereal crops is largely determined by grain size. Here we report the genetic and molecular characterization of GS3, a major quantitative trait locus for grain size. It functions as a negative regulator of grain size and organ size. The wild-type isoform is composed of four putative domains: a plant-specific organ size regulation (OSR) domain in the N terminus, a transmembrane domain, a tumor necrosis factor receptor/nerve growth factor receptor (TNFR/NGFR) family cysteine-rich domain, and a von Willebrand factor type C (VWFC) in the C terminus. These domains function differentially in grain size regulation. The OSR domain is both necessary and sufficient for functioning as a negative regulator. The wild-type allele corresponds to medium grain. Loss of function of OSR results in long grain. The C-terminal TNFR/NGFR and VWFC domains show an inhibitory effect on the OSR function; loss-offunction mutations of these domains produced very short grain. This study linked the functional domains of the GS3 protein to natural variation of grain size in rice.grain weight | Oryza sativa L. | protein domain | yield I n recent years, genes for grain and fruit sizes have been isolated from several plant species (1-8), providing opportunities for understanding genetic and molecular mechanisms regulating these traits. In rice, yield per plant is determined by three component traits: number of panicles (tillers) per plant, number of grains per panicle, and grain weight. Extensive genome mapping studies have identified hundreds of QTLs (quantitative trait loci) for yield traits (9). Although a number of QTLs for tillering (10), grain number (11, 12), grain size (8, 13-15), and panicle size and plant architecture (16-18) have been cloned, molecular characterization of these and many more genes is needed to understand the genetic and molecular bases of yield (9).A major QTL for grain size (GS3) in rice was previously identified on chromosome 3 in a number of studies across different genetic backgrounds and environments (19)(20)(21). Fan et al. (8) identified the candidate gene for GS3. By comparative sequencing analysis, they found a nonsense mutation in the second exon of the putative GS3 shared among all of the large-grain varieties tested in comparison with varieties with smaller grains. This mutation caused a 178-aa truncation in the C terminus of the predicted protein, which was widespread in the global rice germplasm collections (22, 23), indicating that this mutation had an ancient origin and played an important role in grain size variation and domestication of the cultivated rice.Here we report the genetic and molecular analysis of GS3, which revealed several important structural and functional features of the GS3 protein in grain size regulation. ResultsValidation of GS3 on Grain Size Regulation. We validated the effect of GS3 on grain size by transformation. A construct CT9.8 (Fig. S1A), containing a 9.8-kb genomic DNA fragment encompassing GS3 amplified by PCR from rice cultivar Chuan 7 (short grain)...

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A genome-wide analysis of wide compatibility in rice and the precise location of the S5 locus in the molecular map

Cited by 107 publications

References 8 publications

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Untitled

A Simple Genetic Incompatibility Causes Hybrid Male Sterility in Mimulus

Linking differential domain functions of the GS3 protein to natural variation of grain size in rice

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