A genetic map of<i>Cottus gobio</i>(Pisces, Teleostei) based on microsatellites can be linked to the physical map of<i>Tetraodon nigroviridis</i>

Stemshorn, Kathryn; Nolte, Arne W.; Tautz, Diethard

doi:10.1111/j.1420-9101.2005.00929.x

Cited by 35 publications

(36 citation statements)

References 27 publications

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“…These ECRs, which have a mean size of 199 bp, also showed a peculiar nucleotide frequency pattern at their borders, with a strong bias toward A-T (Walter et al 2005). The average size of ECRs identified between pufferfish and sea bream is similar to that reported in other studies, whereas for C. gobio the mean size of conserved regions was shorter (40 bp, Stemshorn et al 2005). The observation of numerous ECRs associated with microsatellite loci might be Two loci (Saimbb13 and Saimbb22) that remained unlinked showed a significant homology, respectively, with Tetraodon chromosome 7 and chromosome 2.…”

Section: Discussionsupporting

confidence: 70%

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A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

et al. 2006

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The gilthead sea bream (Sparus aurata L.) is a marine fish of great importance for fisheries and aquaculture. It has also a peculiar sex-determination system, being a protandrous hermaphrodite. Here we report the construction of a first-generation genetic linkage map for S. aurata, based on 204 microsatellite markers. Twenty-six linkage groups (LG) were found. The total map length was 1241.9 cM. The ratio between sex-specific map lengths was 1:1.2 (male:female). Comparison with a preliminary radiation hybrid (RH) map reveals a good concordance, as all markers located in a single LG are located in a single RH group, except for Ad-25 and CId-31. Comparison with the Tetraodon nigroviridis genome revealed a considerable number of evolutionary conserved regions (ECRs) between the two species. The mean size of ECRs was 182 bp (sequence identity 60-90%). Forty-one ECRs have a known chromosomal location in the pufferfish genome. Despite the limited number of anchoring points, significant syntenic relationships were found. The linkage map presented here provides a robust comparative framework for QTL analysis in S. aurata and is a step toward the identification of genetic loci involved both in the determination of economically important traits and in the individual timing of sex reversal. T HE gilthead sea bream (Sparus aurata L.) is a marine teleost fish that belongs to the family Sparidae. This family contains .100 species, which are divided into two large clades (groups A and B) according to the most recent molecular data (Orrell and Carpenter 2004). Sparids (porgies or sea breams) are demersal fish commonly found in temperate and tropical waters, with a maximum of diversity in the northeast Atlantic and the Mediterranean region (Bauchot and Hureau 1986), and they represent a key element of the coastal marine ecosystem. Sparids are also of great importance for fisheries and aquaculture, being excellent food fish, with high commercial value. S. aurata is the most prominent, with an average cultured production of 100 million metric tons per year. The great importance of the gilthead sea bream for marine aquaculture has fueled an increasing number of studies in many different areas such as immunology, endocrinology, bone morphology, and muscle physiology. Moreover, the genomic tool kit for S. aurata has been constantly improving, as a firstgeneration cDNA microarray was recently reported (Sarropoulou et al. 2005), a preliminary radiation hybrid (RH) map has been constructed (Senger et al. 2006), and a medium-scale expressed sequence tag (EST) sequencing project has been recently completed (A. Canario, personal communication). An improved RH map with .1000 markers (G. Kotoulas, personal communication) and an oligo array representing .10,000 unique transcripts (L. Bargelloni, personal communication) are expected by the end of 2006. Therefore, the genome of the gilthead sea bream is rapidly becoming one of the best characterized among teleost species, apart from classical models such as Brachydanio rerio (zebrafish...

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Section: Discussionsupporting

confidence: 70%

“…Apparently, this is not a general rule in teleost fish. In Cottus gobio, 77/171 (45%) of microsatellite loci could be matched with Tetraodon genomic sequences (Stemshorn et al 2005). In the present study, 62/204 (30%) of loci contain conserved sequence regions compared to the pufferfish genome.…”

Section: Discussionmentioning

confidence: 64%

A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

et al. 2006

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“…BLAST matches were against the T. nigroviridis (Tni), T. rubripes (Tru), G. aculeatus (Gac), O. latipes (Ola) and D. rerio (Dre) genomes using a significance threshold of E , 10 À5 (Stemshorn et al 2005); most of them were retained at E , 10 À10 (in boldface type). ''Unknown'' refers to genome sequences that have not been mapped in Tni.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, unique sequences of the turbot genomic clones containing the microsatellite loci at LG5 were compared by NCBI-BLAST against model fish genomes downloaded from ftp:/ /ftp.ensembl.org: Tetraodon nigroviridis, Takifugu rubripes, Danio rerio, Oryzias latipes, and Gasterosteus aculeatus. Hits were considered significant using a threshold of E , 10 À5 (Stemshorn et al 2005). Population analysis: The SSF broodstock was split by sex for analyzing population parameters at microsatellite loci.…”

Section: Statistical Proceduresmentioning

confidence: 99%

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

et al. 2009

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Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species.

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“…Hits with e , 10 À5 were considered as significant (Stemshorn et al 2005). The T. nigroviridis genome (ver.…”

Section: Methodsmentioning

confidence: 99%

A Microsatellite Genetic Map of the Turbot (Scophthalmus maximus)

et al. 2007

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A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an average distance between markers of 6.5 6 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revealed significant differences between sexes, $1.6 times higher in the female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81-100%). The comparative mapping revealed significant syntenic regions among fish species. This study represents the first linkage map in the turbot, one of the most important flatfish in European aquaculture. This map will be suitable for QTL identification of productive traits in this species and for further evolutionary studies in fish and vertebrate species.

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A genetic map ofCottus gobio(Pisces, Teleostei) based on microsatellites can be linked to the physical map ofTetraodon nigroviridis

Cited by 35 publications

References 27 publications

A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

A Microsatellite Genetic Map of the Turbot (Scophthalmus maximus)

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