A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of fusarium vascular wilt resistance

Abstract

Microsatellites have currently become the markers of choice for molecular mapping and marker-assisted selection for key traits such as disease resistance in many crop species. We report here on the mapping of microsatellites which had been identified from a genomic library of lentil (Lens culinaris Medik.). The majority of microsatellite-bearing clones contained imperfect di-nucleotide repeats. A total of 41 microsatellite and 45 amplified fragment length polymorphism (AFLP) markers were mapped on 86 recombinant inbred lines derived from the cross ILL 5588 x L 692-16-1(s), which had been previously used for the construction of a random amplified polymorphic DNA and AFLP linkage map. Since ILL 5588 was resistant to fusarium vascular wilt caused by the fungus Fusarium oxysporum Shlecht. Emend. Snyder & Hansen f.sp. lentis Vasud. & Srini., the recombinant inbreds were segregating for this character. The resulting map contained 283 markers covering about 751 cM, with an average marker distance of 2.6 cM. The fusarium vascular wilt resistance was localized on linkage group 6, and this resistance gene was flanked by microsatellite marker SSR59-2B and AFLP marker p17m30710 at distances of 8.0 cM and 3.5 cM, respectively. These markers are the most closely linked ones known to date for this agronomically important Fw gene. Using the information obtained in this investigation, the development and mapping of microsatellite markers in the existing map of lentil could be substantially increased, thereby providing the possibility for the future localization of various loci of agronomic interest.

QTLs for iron concentration in seeds of the cultivated lentil (Lens culinaris Medic.) via genotyping by sequencing

“…The total length of the linkage map was 497.1 cM in the current study. In previous lentil mapping studies, lengths of the linkage maps varied from 751 cM to 4060.6 cM (Eujayl et al, 1998;Rubeena et al, 2003;Duran et al, 2004;Hamwieh et al, 2005;Tanyolac et al, 2010;Gupta et al, 2012;De la Puente et al, 2013;Sharpe et al, 2013;Gujaria-Verma, 2014;Ates et al, 2016). The length of the map reported here was shorter than in these previous studies, but the value was close to the findings (751 cM) published by Hamwieh et al (2005).…”

Cross-genera amplification of informative microsatellite markers from common bean and lentil for the assessment of genetic diversity in pigeonpea

“…Biol. Plants, 16(2)-April-June, 2010 based on the sequences reported by Blair et al (2003) and Hamwieh et al (2005), respectively. Primers were custom synthesized from Operon Technologies, USA.…”

“…Despite its importance in nutritional security, pigeonpea has not benefited much from the advances made in the field of genomic tools and molecular markers (Datta et al, 2009). As compared to large number of markers in common bean (L'taief et al, 2008;Blair et al, 2003), chickpea (Choudhary et al, 2009;Sethy et al, 2006), and lentil (Hamwieh et al, 2009;Hamwieh et al, 2005), only 109 microsatellite markers are reported so far in pigeonpea (Burns et al, 2001;Odeny et al, 2007;Odeny et al, 2009). Therefore, there is an urgent need to increase the number of polymorphic microsatellite markers in pigeonpea for diversity analysis and mapping of important traits.…”

Diversification of indigenous gene- pool by using exotic germplasm in lentil (Lens culinaris Medikus subsp. culinaris)

“…Among the various molecular markers, microsatellites or simple sequence repeats (SSR) have shown to be very useful, because these markers showed high polymorphism, reproducible and easy to handle (Varshney et al 2005(Varshney et al , 2009Datta et al 2011). Though in lentil, a number of SSR markers have been developed (Hamwieh et al 2005;Kaur et al 2011;Datta et al 2011), availability of polymorphic SSR markers and their use in analysis of the genetic diversity is still limited in lentil compared to other pulses such as chickpea (Hamwieh et al 2005(Hamwieh et al , 2009Kaur et al 2011;Datta et al 2011). Therefore, present investigation was aimed to assess diversification of Indian gene-pool on the basis of SSR markers and morphological traits among 21 lentil genotypes involving the exotic lines.…”