In this work, the start-ups of air-cathode microbial fuel cells (MFCs) seeds with conventional activated sludge cultivated at different solid retention times (SRTs) are compared. A clear influence of the SRT of the inoculum was observed, corresponding to an SRT of 10 days to the higher current density exerted, about 0.2 A m -2 . This observation points out that, in this type of electrochemical device, it is recommended to use high SRT seeds. The work also points out that in order to promote an efficient start-up, it is not only necessary to use high SRT seeds, but also to feed a high COD concentration. When feeding 10000 ppm COD and keeping SRT of 10 d differences of current densities up to 0.1 A m -2were observed within a cycle. Additionally it was observed that SRT influences direct and indirect electron transfer mechanisms, being the direct mechanisms the most relevant ones, accounting for more than 95% of the total electricity production.