A general method for cell lysis and preparation of deoxyribonucleic acid from streptococci

Monsen, T

doi:10.1016/0378-1097(83)90296-3

Cited by 2 publications

(2 citation statements)

References 7 publications

(11 reference statements)

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“…Bacterial isolates TPC 10, TPC 2.2, TPC 2.3 and Strep. bovis strain 2B were harvested and genomic DNA was isolated by a modification of the Marmur method (Sly et al 1986), incorporating mutanolysin (Monsen et al 1983).…”

Section: S Rdna Sequencingmentioning

confidence: 99%

Streptococcus caprinus sp.nov., a tannin-resistant ruminal bacterium from feral goats

Brooker

O’Donovan

Skene

et al. 1994

Lett Appl Microbiol

132

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An undescribed bacterium capable of clearing tannic acid‐protein complexes has been isolated from ruminal contents of feral goats browsing tannin‐rich Acacia species. The bacterium is a Gram‐positive facultative anaerobe, characterized as a Streptococcus, but DNA‐DNA hybridization and 16S rDNA sequencing show that it is distinct from the common ruminal species Strep. bovis. We propose the name Streptococcus caprinus for this species. The type strain is Strep. caprinus 2.3, Australian Collection of Microorganisms (ACM) 3969. The bacterium grows in media containing at least 2.5% w/v tannic acid or condensed tannin and produces zones of clearing around colonies on nutrient agar plates with added tannic acid. Streptococcus caprinus is not a major inhabitant of domestic livestock, but is found in feral goats browsing tannin‐rich Acacia species, at a population of up to 2 times 106 cfu ml‐1 of rumen fluid.

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Section: S Rdna Sequencingmentioning

confidence: 99%

Streptococcus caprinus sp.nov., a tannin-resistant ruminal bacterium from feral goats

Brooker

O’Donovan

Skene

et al. 1994

Lett Appl Microbiol

132

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show abstract

“…We have developed a rapid screening method for detecting streptococcal plasmids, which works well for preliminary restriction enzyme analysis of these DNA molecules, thus allowing the screening of many transformants within a short time. The method is an adaptation of several procedures used in Escherichia coli [1][2][3][4] and in streptococci [5][6][7][8][9][10]. It employs only a small amount of growth medium and is completed within 4 h; it does not require an ultracentrifugation step.…”

Section: Introductionmentioning

confidence: 99%

A rapid screening method for streptococcal plasmids yielding plasmid DNA suitable for restriction enzyme analysis

Friedrich

1984

FEMS Microbiology Letters

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A general method for cell lysis and preparation of deoxyribonucleic acid from streptococci

Cited by 2 publications

References 7 publications

Streptococcus caprinus sp.nov., a tannin-resistant ruminal bacterium from feral goats

Streptococcus caprinus sp.nov., a tannin-resistant ruminal bacterium from feral goats

A rapid screening method for streptococcal plasmids yielding plasmid DNA suitable for restriction enzyme analysis

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