A gene responsible for vegetative incompatibility in the fungus Podospora anserina encodes a protein with a GTP-binding motif and Gβ homologous domain

Saupe, Sven J.; Turcq, Béatrice; Bégueret, Joël

doi:10.1016/0378-1119(95)00272-8

Cited by 81 publications

(80 citation statements)

References 13 publications

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“…1). We reasoned that as for the het-d and het-e genes, diVerences in the alleles of the het-r locus might lie in the number of WD40 repeats of the WD domain (Saupe et al 1995a(Saupe et al , 1995b; Espagne et al 2002). Therefore, we PCR ampliWed and compared the WD domains of the HNWD1, HNWD2 and HNWD3 loci between the het-R het-V1 and het-r het-V strains.…”

Section: Hnwd2 Shows Length Polymorphism Between Het-r and Het-r Strainsmentioning

confidence: 99%

“…In a number of cases there is now strong evidence that het genes are evolving rapidly (Saupe et al 1996;). These genes are under particular evolutionary regimens characterized by positive diversifying selection and/or balancing selection favouring diversiWcation and maintenance of high levels of polymorphism (Saupe et al 1995a, b;Wu et al 1998;.…”

Section: Introductionmentioning

confidence: 99%

“…het-c encodes for a glycolipid transfer protein (Saupe et al 1994). het-d (Saupe et al 1995a, b) and het-e (Espagne et al 2002) are paralogues, and analysis of the recently sequenced P. anserina genome (Espagne et al 2008) revealed that they belong to a gene family termed HNWD containing Wve members [ Fig. 1; ].…”

Section: Introductionmentioning

confidence: 99%

“…The sequence of the WD domain of het-d and het-e deWnes the speciWcity of interaction with het-C alleles (Saupe et al 1995a, b;Espagne et al 2002). We have previously reported that a combination of original evolutionary mechanisms governs the generation of extensive polymorphism at loci of the HNWD family.…”

Section: Introductionmentioning

confidence: 99%

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Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family

et al. 2009

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In fungi, vegetative incompatibility is a conspeciWc non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of speciWc het loci interact. In Podospora anserina, three nonallelic incompatibility systems have been genetically deWned involving interactions between het-c and het-d, het-c and het-e, het-r and het-v. het-d and het-e are paralogues belonging to the HNWD gene family that encode proteins of the STAND class. HET-D and HET-E proteins comprise an N-terminal HET eVector domain, a central GTP binding site and a C-terminal WD repeat domain constituted of tandem repeats of highly conserved WD40 repeat units that deWne the speciWcity of alleles during incompatibility. The WD40 repeat units of the members of this HNWD family are undergoing concerted evolution. By combining genetic analysis and gain of function experiments, we demonstrate that an additional member of this family, HNWD2, corresponds to the het-r non-allelic incompatibility gene. As for het-d and het-e, allele speciWcity at the het-r locus is determined by the WD repeat domain. Natural isolates show allelic variation for het-r.

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Section: Hnwd2 Shows Length Polymorphism Between Het-r and Het-r Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family

et al. 2009

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“…Vegetative incompatibility regulates the fusion of hyphae, which is generally followed by the exchange of cytoplasm and nuclei. ESTs displaying similarity to the Podospora anserina vegetative incompatibility protein HET-E-1 were identified (Saupe et al, 1995). Finally, an EST with similarity to a novel antifungal peptide presumed to be an important element of the innate immunity system from the insect Acrocinus longimanus (Coleoptera) was identified (Barbault et al, 2003).…”

Section: Functional Analysis Of the Est Librariesmentioning

confidence: 99%

EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia

et al. 2006

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The entomopathogenic fungus Beauveria (Cordyceps) bassiana holds much promise as a pest biological control agent. B. bassiana produces at least three in vitro single cell infectious propagules, including aerial conidia, vegetative cells termed blastospores and submerged conidia, that display different morphological, biochemical and virulence properties. Populations of aerial conidia, blastospores and submerged conidia were produced on agar plates, rich liquid broth cultures and under conditions of nutrient limitation in submerged cultures, respectively. cDNA libraries were generated from mRNA isolated from each B. bassiana cell type and ∼2500 5′ end sequences were determined from each library. Sequences derived from aerial conidia clustered into 284 contigs and 963 singlets, with those derived from blastospores and submerged conidia forming 327 contigs with 788 singlets, and 303 contigs and 1079 contigs, respectively. Almost half (40–45 %) of the sequences in each library displayed either no significant similarity (e value >10−4) or similarity to hypothetical proteins found in the NCBI database. The expressed sequence tag dataset also included sequences representing a significant portion of proteins in cellular metabolism, information storage and processing, transport and cell processes, including cell division and posttranslational modifications. Transcripts encoding a diverse array of pathogenicity-related genes, including proteases, lipases, esterases, phosphatases and enzymes producing toxic secondary metabolites, were also identified. Comparative analysis between the libraries identified 2416 unique sequences, of which 20–30 % were unique to each library, and only ∼6 % of the sequences were shared between all three libraries. The unique and divergent representation of the B. bassiana transcriptome in the cDNA libraries from each cell type suggests robust differential gene expression profiles in response to environmental conditions.

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Microscopic and Ultrastructural Examination of Vegetative Incompatibility in Partial Diploids Heterozygous athetLoci inNeurospora crassa

Jacobson

Beurkens

Klomparens

1998

Fungal Genetics and Biology

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A gene responsible for vegetative incompatibility in the fungus Podospora anserina encodes a protein with a GTP-binding motif and Gβ homologous domain

Cited by 81 publications

References 13 publications

Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family

Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family

EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia

Microscopic and Ultrastructural Examination of Vegetative Incompatibility in Partial Diploids Heterozygous athetLoci inNeurospora crassa

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