Microscopic and Ultrastructural Examination of Vegetative Incompatibility in Partial Diploids Heterozygous athetLoci inNeurospora crassa

Jacobson, David J.; Beurkens, K.; Klomparens, Karen L.

doi:10.1006/fgbi.1997.1020

Cited by 77 publications

(83 citation statements)

References 29 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many researchers suggest that a filamentous fungus cannot form heterokaryons with genetically similar ones (Worral, 1997;Debets, 1998;Jacobson et al, 1998). In the present study, crossing between strains IB16/02 and Cs14 was observed, which have similar genetic constitution according to RAPD analysis.…”

Section: Resultssupporting

confidence: 61%

Genetic variability and vegetative compatibility of Erythricium salmonicolor isolates

Sebastianes

Maki

Andreote

et al. 2007

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

The Pink Disease is caused by Erythricium salmonicolor, which attacks broad hosts, such as citrus, coffee, rubber, Eucalyptus spp. and Acacia spp., infecting mainly branches. This disease became a serious problem in Brazil, reducing the citrus production up to 10%. However the genetic diversity and compatibility of the fungus E. salmonicolor from Brazilian citrus plants is not yet evaluated. Therefore, the aims of this study were to evaluate: i) the genetic variability of E. salmonicolor in the São Paulo and Minas Gerais States by the RAPD technique, and ii) the vegetative compatibility between these isolates. After RAPD analysis, six distinct groups were observed without correlation between the isolation site or host species. In the vegetative compatibility test, the contact of fungal hyphae between all evaluated crosses was observed, of which 84% presented hyphal fusion. Although the compatibility between strains was observed, no correlation between RAPD haplotypes and hyphal anastomosis was verified. These results show the importance of future studies on the sexual cycle of E. salmonicolor, since hyphal fusion, which precedes the formation of heterokaryons (sexual and parasexual reproduction) that could be responsible for the genetic variability in this species.

show abstract

Section: Resultssupporting

confidence: 61%

Genetic variability and vegetative compatibility of Erythricium salmonicolor isolates

Sebastianes

Maki

Andreote

et al. 2007

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

show abstract

“…Interactions between genetically distinct individuals of the same species result in an incompatible reaction that triggers localized programmed cell death (PCD), forming a line of demarcation, or barrage, along the zone of contact (10)(11)(12) and restricting cytoplasmic transmission of viruses and other cytoplasmic elements (1,(13)(14)(15).…”

mentioning

confidence: 99%

Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes

Zhang

Nuss

2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Transmission of mycoviruses that attenuate virulence (hypovirulence) of pathogenic fungi is restricted by allorecognition systems operating in their fungal hosts. We report the use of systematic molecular gene disruption and classical genetics for engineering fungal hosts with superior virus transmission capabilities. Four of five diallelic virus-restricting allorecognition [vegetative incompatibility (vic)] loci were disrupted in the chestnut blight fungus Cryphonectria parasitica using an adapted Cre-loxP recombination system that allowed excision and recycling of selectable marker genes (SMGs). SMG-free, quadruple vic mutant strains representing both allelic backgrounds of the remaining vic locus were then produced through mating. In combination, these super donor strains were able to transmit hypoviruses to strains that were heteroallelic at one or all of the virus-restricting vic loci. These results demonstrate the feasibility of modulating allorecognition to engineer pathogenic fungi for more efficient transmission of virulence-attenuating mycoviruses and enhanced biological control potential.M ycovirus infections have been reported to reduce virulence (hypovirulence) for a wide range of plant pathogenic fungi, providing potential for biological disease control (1-6). For hypovirulence to be effective, the virulence-attenuating viruses must be efficiently transmitted from infected hypovirulent strains to uninfected virulent strains (5, 7). Mycoviruses generally have evolved exclusive intracellular lifestyles (8). With very few exceptions (9), mycovirus infections cannot be initiated by exposure of uninfected hyphae to cell extracts or secretions from infected fungal isolates. Transmission is limited to intracellular mechanisms, vertical transmission through asexual spores, and horizontal transmission through anastomosis (fusion of hyphae).Horizontal mycovirus transmission to uninfected isolates of the same fungal species is complicated by nonself allorecognition genetic systems, termed "heterokaryon" or "vegetative incompatibility" (vic), which operate widely in filamentous fungi (10). Interactions between genetically distinct individuals of the same species result in an incompatible reaction that triggers localized programmed cell death (PCD), forming a line of demarcation, or barrage, along the zone of contact (10-12) and restricting cytoplasmic transmission of viruses and other cytoplasmic elements (1,(13)(14)(15).A negative correlation between vic diversity and virus transmission has been reported for several fungal hosts (1, 16), but has most extensively been demonstrated for the chestnut blight fungus Cryphonectria parasitica infected with virulence-attenuating hypoviruses (7,(17)(18)(19)(20). Genetic analyses have defined six diallelic vic genetic loci for C. parasitica (21). These loci and associated genes were recently identified at the molecular level through a comparative genomics approach (22, 23) ( Table 1). Independent gene disruption analysis of 12 genes associated with these loci (22, 23) ...

show abstract

“…To establish if PCD is associated with vegetative incompatibility in A. areolatum, Evans blue (Jacobson et al, 1998) and Giemsa (Hutchison et al, 2009) staining were employed. Evans blue penetrates only dead cells and it was used it to compare the proportion of dead compartments between compatible and incompatible interactions.…”

Section: Evans Blue Giemsa and α-Naphthol Stainingmentioning

confidence: 99%

“…Three days after hyphal contact, segments of hyphae of the interaction or contact zone were positioned on microscope slides after which the fungal tissue was stained with Evans blue (0.05 %; Sigma, St. Louis, MO) for 45 minutes at room temperature and washed with phosphate buffered saline (Jacobson et al, 1998). A Zeiss Axiocam light microscope using Zeis software (Zeis, Germany) was then used to determine the proportion of dead compartments.…”

Section: Evans Blue Giemsa and α-Naphthol Stainingmentioning

confidence: 99%

Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Nest¹,

Steenkamp²,

Slippers³

et al. 2011

Fungal Genetics and Biology

View full text Add to dashboard Cite

In filamentous fungi, vegetative compatibility among individuals of the same species is determined by the genes encoded at the heterokaryon incompatibility (het) loci. The hyphae of genetically similar individuals that share the same allelic specificities at their het loci are able to fuse and intermingle, while different allelic specificities at the het loci result in cell death of the interacting hyphae. In this study, suppression subtractive hybridization (SSH) followed by pyrosequencing and quantitative reverse transcription PCR were used to identify genes that are selectively expressed when vegetatively incompatible individuals of Amylostereum areolatum interact. The SSH library contained genes associated with various cellular processes, including cell-cell adhesion, stress and defence responses, as well as cell death. Some of the transcripts encoded proteins that were previously implicated in the stress and defence responses associated with vegetative incompatibility. Other transcripts encoded proteins known to be associated with programmed cell death, but have not previously been linked with vegetative incompatibility.Results of this study have considerably increased our knowledge of the processes underlying vegetative incompatibility in Basidiomycetes in general and A. areolatum in particular.

show abstract

Microscopic and Ultrastructural Examination of Vegetative Incompatibility in Partial Diploids Heterozygous athetLoci inNeurospora crassa

Cited by 77 publications

References 29 publications

Genetic variability and vegetative compatibility of Erythricium salmonicolor isolates

Genetic variability and vegetative compatibility of Erythricium salmonicolor isolates

Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes

Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Contact Info

Product

Resources

About