A gene family homologous to the S‐phase specific gene in higher plants is essential for cell proliferation in <i>Saccharomyces cerevisiae</i>

Ito, Masaki; Yasui, Akira; Komamine, Atsushi

doi:10.1016/0014-5793(92)80203-s

Cited by 15 publications

(24 citation statements)

References 13 publications

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“…Therefore, the decrease in arsenic accumulation by Ntcyc07 expression is not due to altered cell growth or division, suggesting that expression of Ntcyc07 promotes tolerance by reducing the intracellular levels of arsenic. Although the disruption of the genes for two cyc07 homologues inhibited cell proliferation in S. cerevisiae [26], our results imply that over-expression of foreign (tobacco) cyc07 may not affect cell division in yeast. Over-expression of cyc07 has never been found to influence cell proliferation in any organism.…”

Section: Cell Division and Cell Size Were Not Altered In Yeast Cellsmentioning

confidence: 54%

“…Is there a relationship between cell division and metalloid accumulation? To examine the idea that cyc07 might play a role in cell proliferation in S. cerevisiae [26], growth rates of Y800-pYES5 and Y800-Ntcyc07 cells in ura(À) CM medium were compared with and without As(III). Y800-pYES5 and Y800-Ntcyc07 cells grew at similar rates in CM medium without arsenite, but Y800-Ntcyc07 cells grew faster in CM medium with 400 lM arsenite, whereas Y800-pYES5 did not grow at all (Fig.…”

Section: Cell Division and Cell Size Were Not Altered In Yeast Cellsmentioning

confidence: 99%

“…Cyc07 mRNA was detected specifically in the S phase of synchronous cultures as well as in intact plants and was found preferentially in the meristematic tissue of root tips [25]. Two yeast genes encoding homologues of Cyc07 are essential for cell proliferation in Saccharomyces cerevisiae [26]. Expression of an Oryza sativa homologue of Cyc07 is regulated by external stresses such as high osmotic pressure, salinity, low temperature and submergence [27].…”

Section: Introductionmentioning

confidence: 99%

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The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

et al. 2008

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We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1.

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Section: Cell Division and Cell Size Were Not Altered In Yeast Cellsmentioning

confidence: 54%

Section: Cell Division and Cell Size Were Not Altered In Yeast Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

et al. 2008

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“…1B) did not show the ts phenotype [3]. Furthermore, we found another ORF (ORF-2) located downstream from PLC2 gene with an opposite orientation to PLC2, which was partially con- tamed in the PstI 2.3 kb fragment (Fig.…”

Section: Introductionmentioning

confidence: 83%

“…In our previous work, the yeast, Saccharomyces cerevisiae, was found to have a gene family constituted by two closely related genes, namely PLCI and PLC2, which were homologous to cycU7 from C. roseus. The lethal phenotype resulted from a double PLCl and PLC2 gene disruption suggesting an important role for this gene in cell cycle progression [3]. The gene sequence that is identical to PLC2 has recently been isolated from the yeast, S. cerevisiue by Garrett et al [4].…”

Section: Introductionmentioning

confidence: 99%

Precise mapping and molecular characterization of the MFT1 gene involved in import of a fusion protein into mitochondria in Saccharomyces cerevisiae

1993

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Garrett et al. [Mol. Gen. Genet. 225 (1991) 483‐491] recently reported that an Atp2‐lacZ fusion protein was transported into mitochondria in yeast, thus identifying the MFT1 (mitochondrial fusion targeting) gene as a genomic fragment which complements a mutation (mftI) that failed in targeting a fusion protein into mitochondria [4]. They mapped this gene to the ORF, which we have independently identified as a gene homologous to the cyc07 gene, which is expressed specifically in the S phase during the plant cell cycle. We have mapped the MFT1 gene precisely and found that this gene should correspond to the neighboring ORF, rather than the ORF they identified.

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Meristem-specific gene expression directed by the promoter of the S-phase-specific gene, cyc07, in transgenic Arabidopsis

et al. 1994

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A genomic clone for the cyc07 gene, which is expressed specifically at the S phase during the cell cycle in synchronous cultures of periwinkle (Catharanthus roseus) cells, was isolated. Determination of the nucleotide sequence of the clone revealed that the cyc07 gene consists of seven exons separated by six introns. Genomic Southern analysis indicated that the cyc07 gene is present as a single copy per haploid genome in periwinkle. Expression of related genes was detected in a wide range of other plants. Transgenic Arabidopsis plants were generated that expressed the gene for beta-glucuronidase (GUS) under the control of the promoter of the cyc07 gene. The tissue-specific pattern of expression directed by the promoter was investigated by analysis of GUS activity. Histochemical tests demonstrated that 589 bp of the 5'-upstream sequence of the cyc07 gene could direct specifical expression of the GUS reporter gene in meristematic tissues in transgenic plants. The spatial pattern of expression directed by the promoter was closely correlated with meristematic activity and cell proliferation, suggesting an association between the function of the cyc07 gene and cell proliferation.

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A gene family homologous to the S‐phase specific gene in higher plants is essential for cell proliferation in Saccharomyces cerevisiae

Cited by 15 publications

References 13 publications

The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

Precise mapping and molecular characterization of the MFT1 gene involved in import of a fusion protein into mitochondria in Saccharomyces cerevisiae

Meristem-specific gene expression directed by the promoter of the S-phase-specific gene, cyc07, in transgenic Arabidopsis

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