A gel-free proteomic analysis of Taenia solium and Taenia crassiceps cysticerci vesicular extracts

Abstract

The taeniasis/cysticercosis complex is a zoonosis caused by the presence of the parasite Taenia solium in humans. It is considered a neglected disease that causes serious public health and economic problems in developing countries. In humans, the most common locations for the larval form are the skeletal muscles, ocular system, and the central nervous system, which is the most clinically important. Several glycoproteins of T. solium and Taenia crassiceps cysticerci have been characterized and studied for their use in the immunodiagnosis of neurocysticercosis and/or the development of synthetic or recombinant vaccines against cysticercosis. The aim of this study was to perform a gel-free shotgun proteomic analysis to identify saline vesicular extract (SVE) proteins of T. solium and T. crassiceps cysticerci. After solubilization of the SVE with and without surfactant reagent and in-solution digestion, the proteins were analyzed by LC-MS/MS. Use of a surfactant resulted in a significantly higher number of proteins that were able to be identified by LC-MS/MS. Novel proteins were identified in T. solium and T. crassiceps SVE. The qualitative analysis revealed a total of 79 proteins in the Taenia species: 29 in T. solium alone, 11 in T. crassiceps alone, and 39 in both. These results are an important contribution to support future investigations and for establishing a Taenia proteomic profile to study candidate biomarkers involved in the diagnosis or pathogenesis of neurocysticercosis.

Proteomic analysis of Taenia solium cysticercus and adult stages

“…Proteomics of worms had been shown to understand their biology/pathogenesis, diagnostic biomarkers, new drug targets, or potential vaccine candidates. Several reports that investigated the proteome of T. solium across its developmental stages have discovered that cytoskeleton, actin, and paramyosin can be used as targets for applied research on cysticercosis vaccines and potential diagnostic antigens (e.g., enolase, calcium-binding protein, small molecule shock protein, 14-3-3 protein, tropomyosin α, α-1 tubulin, β tubulin, annexin B1, and cAMP cyclic adenylate protein-dependent kinase) ( 11 – 14 ). However, we expect to increase our knowledge of their pathogenesis through the T. solium and cysticercus proteomes.…”

Comparative Proteomic Analysis of Taenia solium Cysticercus and Adult Stages

“…Identi ed Proteins of Cysticercus (Down-regulated) Several reports that investigated the proteome of T. solium across its developmental stages have discovered that cytoskeleton, actin, and paramyosin could be used as targets for applied research on cysticercosis vaccines and potential diagnostic antigens (e.g., enolase, calcium binding protein, small molecule shock protein, 14-3-3 protein, tropomyosin α, α-1 tubulin, β tubulin, annexin B1 and cAMP cyclic adenylate protein dependent kinase) [16][17][18][19]. Calcium-binding protein was involved in various regulatory functions of host invasion by parasites, these mainly including members of the calmodulin family (CAM), the calcineurin B-like (CBL) family, and the calcium-dependent protein kinase (CDPK) family: all these harbor a highly conserved helix-loop-helix structure, namely the EF-hand domain.…”

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

“…So far, to our knowledge, proteomics has described the fallowing main Taenia spp. features: total protein composition of cysticerci of T. solium by 2-DE [ 196 ]; in T. solium, using LC-MS/MS, a set of oncosphere proteins involved in gut penetration and immune evasion machineries in adhesion [ 197 ]; candidate antigens through immunoproteomics [ 198 , 199 , 200 , 201 ]; T. solium cysts proteomes obtained from different host tissues [ 202 , 203 ]; saline vesicular extract proteins of T. solium [ 204 ]; and T. solium excretory-secretory proteome [ 205 ].…”