A gel-free multi-well microfluidic device utilizing surface tension for cell culturing

Shih, Ming-Cheng; Tseng, Shih-Heng; Weng, Yu-Shih; Chu, I‐Ming; Liu, Cheng-Hsien

doi:10.1016/j.snb.2012.11.001

Cited by 3 publications

(4 citation statements)

References 42 publications

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“…In the microfluidic system, the fluid has three characteristics including extremely efficient mass and heat exchange, domination of fluid resistance on fluid behavior, and a significant influence of surface tension . The controlled delivery systems can achieve precise manipulation of the individual fluid through making full use of the three characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…11 In the microfluidic system, the fluid has three characteristics including extremely efficient mass and heat exchange, domination of fluid resistance on fluid behavior, and a significant influence of surface tension. 12 The controlled delivery systems can achieve precise manipulation of the individual fluid through making full use of the three characteristics. The microparticle-based materials are prepared by microfluidic methods that have controllable morphology and uniform composition, which brings convenience to quality evaluation and applications in the fields of nutraceutical delivery.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Assembly: An Innovative Tool for the Encapsulation, Protection, and Controlled Release of Nutraceuticals

Liu

Singh

Zhang

et al. 2021

J. Agric. Food Chem.

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Nutraceuticals have been gradually accepted as food ingredients that can offer health benefits and provide protection against several diseases. It is widely accepted due to potential nutritional benefits, safety, and therapeutic effects. Most nutraceuticals are vulnerable to the changes in the external environment, which leads to poor physical and chemical stability and absorption. Several researchers have designed various encapsulation technologies to promote the use of nutraceuticals. Microfluidic technology is an emerging approach which can be used for nutraceutical delivery with precise control. The delivery systems using microfluidic technology have obtained much interest in recent years. In this review article, we have summarized the recently introduced nutraceutical delivery platforms including emulsions, liposomes, microspheres, microgels, and polymer nanoparticles based on microfluidic techniques. Emphasis has been made to discuss the advantages, preparations, characterizations, and applications of nutraceutical delivery systems. Finally, the challenges, several up-scaling methods, and future expectations are discussed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic Assembly: An Innovative Tool for the Encapsulation, Protection, and Controlled Release of Nutraceuticals

Liu

Singh

Zhang

et al. 2021

J. Agric. Food Chem.

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“…The formation of gap junctions and extended bile canaliculi during in vitro hepatocyte culturing indicated that a 3D microenvironment could be induced in the absence of ECM provided with dense cell-cell interactions on a perfused microfluidic platform. More recently, another hepatocyte culturing technique utilizing gel-free microfluidic platforms was presented [ 36 ]. The authors proposed a multi-row square-pillar microstructure as the perfusion mechanism with a larger cell culture area.…”

Section: Livermentioning

confidence: 99%

Cell Patterning for Liver Tissue Engineering via Dielectrophoretic Mechanisms

Yahya

Kadri

Ibrahim

2014

Sensors

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Liver transplantation is the most common treatment for patients with end-stage liver failure. However, liver transplantation is greatly limited by a shortage of donors. Liver tissue engineering may offer an alternative by providing an implantable engineered liver. Currently, diverse types of engineering approaches for in vitro liver cell culture are available, including scaffold-based methods, microfluidic platforms, and micropatterning techniques. Active cell patterning via dielectrophoretic (DEP) force showed some advantages over other methods, including high speed, ease of handling, high precision and being label-free. This article summarizes liver function and regenerative mechanisms for better understanding in developing engineered liver. We then review recent advances in liver tissue engineering techniques and focus on DEP-based cell patterning, including microelectrode design and patterning configuration.

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“…pillar structures) in microfluidic devices can induce significant flow deformation and migration of microparticles or cells. 22,23 Based on the addressed challenges and adopting structural benefits from previous reports, the incorporation of microstructures into a microfluidic device could be an alternative approach to separate, 24,25 isolate, 21 and trap 26 the agglomerated microbials. Additionally, the currently developed droplet-based microfluidic system provides an ideal platform for isolation of cells in hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Micropillar arrays enabling single microbial cell encapsulation in hydrogels

et al. 2014

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Single microbial cell encapsulation in hydrogels is an important task to find valuable biological resources for human welfare. The conventional microfluidic designs are mainly targeted only for highly dispersed spherical bioparticles. Advanced structures should be taken into consideration for handling such aggregated and non-spherical microorganisms. Here, to address the challenge, we propose a new type of cylindrical-shaped micropillar array in a microfluidic device for enhancing the dispersion of cell clusters and the isolation of individual cells into individual micro-hydrogels for potential practical applications. The incorporated micropillars act as a sieve for the breaking of Escherichia coli (E. coli) clusters into single cells in a polymer mixture. Furthermore, the combination of hydrodynamic forces and a flow-focusing technique will improve the probability of encapsulation of a single cell into each hydrogel with a broad range of cell concentrations. This proposed strategy and device would be a useful platform for genetically modified microorganisms for practical applications.

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A gel-free multi-well microfluidic device utilizing surface tension for cell culturing

Cited by 3 publications

References 42 publications

Microfluidic Assembly: An Innovative Tool for the Encapsulation, Protection, and Controlled Release of Nutraceuticals

Microfluidic Assembly: An Innovative Tool for the Encapsulation, Protection, and Controlled Release of Nutraceuticals

Cell Patterning for Liver Tissue Engineering via Dielectrophoretic Mechanisms

Micropillar arrays enabling single microbial cell encapsulation in hydrogels

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