Cell Patterning for Liver Tissue Engineering via Dielectrophoretic Mechanisms

Yahya, Wan Nurlina Wan; Kadri, Nahrizul Adib; Ibrahim, Fatimah

doi:10.3390/s140711714

Cited by 16 publications

(11 citation statements)

References 91 publications

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“…Sample preparation is an essential step in clinical diagnostic applications, which includes centrifugation, extraction, concentration, chemical reactions, washing, and other laborious practices . Various technologies have been developed for sample preparation prior to analysis using microfluidic systems; however, dielectrophoresis (DEP) has proven to be a promising technique for the manipulation of micro/nanoscale objects, including cells, viruses, DNA, bacteria, and proteins, in aqueous suspensions .…”

Section: Introductionmentioning

confidence: 99%

Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

et al. 2015

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Dielectrophoresis (DEP), the induced movement of dielectric particles placed in a nonuniform electric field, has been used as a potential technique for manipulation and separation of many biological samples without destructive consequences to the cell. Cells of the same genotype in different physiological and pathological states have unique morphological and structural features, therefore, it is possible to differentiate between them using their DEP responses. This paper reports the experimental discrimination of normal and dengue-infected human hepatic fetal epithelial cells (WRL-68 cells) based on their DEP crossover frequency, at which no resultant movement occurs in the cells in response to the DEP force. A microarray dot electrode was used to conduct the DEP experiments. The DEP forces applied to the cells were quantified by analyzing the light intensity shift within the electrode's dot region based on the Cumulative Modal Intensity Shift image analysis technique. The differences in dielectric properties between infected and uninfected cells were exploited by plotting a unique DEP spectrum for each set of cells. We observed that the crossover frequency decreased from 220 kHz for the normal WRL-68 cells to 140 kHz after infection with the dengue virus in a medium conductivity of 100 μS/cm. We conclude that the change in the DEP crossover frequency between dengue-infected cells and their healthy counterparts should allow direct characterization of these cell types by exploiting their electrophysiological properties.

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Section: Introductionmentioning

confidence: 99%

Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

et al. 2015

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“…Dielectrophoresis (DEP) has been used to detect bacteria [ 1 ], measure the electric properties of cells [ 2 , 3 ], and to form cell patterns [ 4 , 5 , 6 , 7 , 8 ] to apply to biomedical sciences [ 9 ]. Additionally, it has been especially studied as an external force for separating cells in microfluidic devices [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Separation of Blood Cells Based on the Negative Dielectrophoresis Operated by Three Dimensional Microband Electrodes

et al. 2020

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A microfluidic device is presented for the continuous separation of red blood cells (RBCs) and white blood cells (WBCs) in a label-free manner based on negative dielectrophoresis (n-DEP). An alteration of the electric field, generated by pairs of slanted electrodes (separators) that is fabricated by covering parts of single slanted electrodes with an insulating layer is used to separate cells by their sizes. The repulsive force of n-DEP formed by slanted electrodes prepared on both the top and bottom substrates led to the deflection of the cell flow in lateral directions. The presence of gaps covered with an insulating layer for the electric field on the electrodes allows the passing of RBCs through gaps, while relatively large WBCs (cultured cultured human acute monocytic leukemia cell line (THP-1 cells)) flowed along the slanted separator without passing through the gaps and arrived at an edge in the channel. The passage efficiency for RBCs through the gaps and the arrival efficiency for THP-1 cells to the upper edge in the channel were estimated and found to be 91% and 93%, respectively.

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“…Almost all dielectric particles can be subjected to dielectrophoresis. Dielectrophoresis find application in clinical diagnostics, disease treatment, pharmacology, immunology, biotechnology, separation of cell cultures and others .…”

Section: Introductionmentioning

confidence: 99%

Barrier contactless dielectrophoresis: A new approach to particle separation

Podoynitsyn

Sorokina

Klimov

et al. 2019

Separation Science Plus

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Barrier contactless dielectrophoresis is proposed as a method for microbiological particle separation. Design of a separation device for barrier contactless dielectrophoresis is discussed. The principle of separation device is based on formation of barriers of gradient fields in the volume of separation chamber by electrodes with passivation dielectric coating of silicon carbide. A feature of the method is absence of contact between separated samples and conductive elements of electrode structure. The proposed method is highly efficient and easy to use in comparison with dielectrophoresis on isolated structures and conventional planar systems. Computer simulation of electric field distribution over dielectrophoretic barriers is carried out, and effect of the thickness of the passivation coating on dielectrophoretic properties of barriers is shown. Forces acting on a particle (a yeast cell with a diameter of 7 μm) in separation chamber are evaluated using computer simulation data. Ability of the cell to be captured by the method of contactless barrier dielectrophoresis is theoretically predicted and confirmed experimentally, using yeast cells. The yeast cells subjected to positive dielectrophoresis can be effectively captured (up to 90%) by the separation device at the applied voltage of 20 V and 100 kHz and at flow rate of 20 mL/h.

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Cell Patterning for Liver Tissue Engineering via Dielectrophoretic Mechanisms

Cited by 16 publications

References 91 publications

Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

Microfluidic Separation of Blood Cells Based on the Negative Dielectrophoresis Operated by Three Dimensional Microband Electrodes

Barrier contactless dielectrophoresis: A new approach to particle separation

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