A gain‐of‐function mutation of plastidic invertase alters nuclear gene expression with sucrose treatment partially via <scp>GENOMES UNCOUPLED</scp>1‐mediated signaling

Maruta, Takanori; Miyazaki, Nozomi; Nosaka, Ryota; Tanaka, Hiroyuki; Padilla‐Chacón, Daniel; Otori, Kumi; Kimura, Ayako; Tanabe, Noriaki; Yoshimura, Kazuya; Tamoi, Masahiro; Shigeoka, Shigeru

doi:10.1111/nph.13309

Cited by 14 publications

(13 citation statements)

References 42 publications

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“…These observations are in line with the model suggesting that plastid gene transcription is controlled by retrograde signalling networks including sigma factors (SIG2, SIG6) and plastid encoded RNA polymerase (PEP) which might be crucial for proper plastid RNA transcription (Woodson et al, 2013). It seems that GUN1 activates PEP (Maruta et al, 2015) and a perturbed PEP activation in the gun1 mutant may prevent the upregulation of the plastid encoded genes in the treated gun1 mutant compared to the treated wild type.…”

Section: Discussionsupporting

confidence: 86%

Impact of small RNAs in retrograde signalling pathways inArabidopsis thaliana

Habermann

Tiwari

Krantz

et al. 2019

Preprint

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Chloroplast perturbations activate retrograde signalling pathways causing dynamic changes of gene expression. Besides transcriptional control of gene expression different classes of small non-coding RNAs (sRNAs) act in gene expression control, but comprehensive analyses regarding their role in retrograde signalling is lacking. We performed sRNA profiling in response to norflurazon (NF) that provokes retrograde signals in A. thaliana wild type and the two retrograde signalling mutants gun1 and gun5. The RNA samples were also used for mRNA and long non-coding RNA (lncRNA) profiling to link altered sRNA levels to changes of their cognate target RNAs. We identified 122 sRNAs from all known sRNA classes that were responsive to NF in wild type. Strikingly, 140 and 213 sRNAs were found to be differentially regulated in both mutants indicating a retrograde control of these sRNAs. Concomitant with the changes in sRNA expression we detected about 1500 differentially expressed mRNAs in the NF treated wild type and around 900 and 1400 mRNAs that were differentially regulated in the gun1 and gun5 mutant with a high proportion (~30%) of genes encoding plastid proteins. Furthermore, around 20% of predicted miRNA targets code for plastid localised proteins. The analyses of sRNA-target pairs identified pairs with an anticorrelated expression as well pairs showing other expressional relations pointing to a role of sRNAs in balancing transcriptional changes upon retrograde signals. Based on the comprehensive changes in sRNA expression we assume a considerable impact of sRNAs in retrograde-dependent transcriptional changes to adjust plastidic and nuclear gene expression. Significance statementPerturbations of plastid functions trigger retrograde signalling to adjust plastidic and nuclear gene expression, however, the role of small non-coding RNAs acting as regulators in these pathways is not well understood. We analysed small non-coding RNA expression in response to retrograde signals in A. thaliana wild type and two retrograde signalling mutants and identified members of all known small non-coding RNA classes pointing to a functional role of these RNA classes in retrograde pathways.

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Section: Discussionsupporting

confidence: 86%

Impact of small RNAs in retrograde signalling pathways inArabidopsis thaliana

Habermann

Tiwari

Krantz

et al. 2019

Preprint

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“…The signaling pathway by which the status of the developing chloroplast is relayed to the nucleus is one of the few remaining plant signaling pathways that we know of, but for which we have little idea of the signaling components involved. We believe this study resolves one of the major discrepancies in plastid signaling research by eliminating a major role for PTM, and paves the way for more focused studies that build on recent progress on the role of tetrapyrroles and chloroplast protein homeostasis in plastid retrograde signaling (Woodson et al, 2011;Maruta et al, 2015;Ibata et al, 2016;Tadini et al, 2016).…”

mentioning

confidence: 66%

Seedlings Lacking the PTM Protein Do Not Show a genomes uncoupled (gun) Mutant Phenotype

et al. 2017

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“…In this study, gene expression analysis showed that the lpe2 mutation generated a retrograde signal, thus altering the expression of several different kinds of genes (Figure 4), including those involved in C and N response ( Figure 5). Since the expression of C and N response genes is regulated by C/N balance (Oliveira and Coruzzi 1999;Martin et al 2002;Maruta et al 2015), our data suggest that LPE2 is also involved in C/N balance. This was supported by the C/N stress test, in which the loss of LPE2 suppressed C/N response ( Figure 6).…”

Section: Discussionmentioning

confidence: 75%

Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana

Dong

Duan

Wang

et al. 2020

JIPB

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The balance between cellular carbon (C) and nitrogen (N) must be tightly coordinated to sustain optimal growth and development in plants. In chloroplasts, photosynthesis converts inorganic C to organic C, which is important for maintenance of C content in plant cells. However, little is known about the role of chloroplasts in C/N balance. Here, we identified a nuclear‐encoded protein LOW PHOTOSYNTHETIC EFFICIENCY2 (LPE2) that it is required for photosynthesis and C/N balance in Arabidopsis. LPE2 is specifically localized in the chloroplast. Both loss‐of‐function mutants, lpe2‐1 and lpe2‐2, showed lower photosynthetic activity, characterized by slower electron transport and lower PSII quantum yield than the wild type. Notably, LPE2 is predicted to encode the plastid ribosomal protein S21 (RPS21). Deficiency of LPE2 significantly perturbed the thylakoid membrane composition and plastid protein accumulation, although the transcription of plastid genes is not affected obviously. More interestingly, transcriptome analysis indicated that the loss of LPE2 altered the expression of C and N response related genes in nucleus, which is confirmed by quantitative real‐time‐polymerase chain reaction. Moreover, deficiency of LPE2 suppressed the response of C/N balance in physiological level. Taken together, our findings suggest that LPE2 plays dual roles in photosynthesis and the response to C/N balance.

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A gain‐of‐function mutation of plastidic invertase alters nuclear gene expression with sucrose treatment partially via GENOMES UNCOUPLED1‐mediated signaling

Cited by 14 publications

References 42 publications

Impact of small RNAs in retrograde signalling pathways inArabidopsis thaliana

Impact of small RNAs in retrograde signalling pathways inArabidopsis thaliana

Seedlings Lacking the PTM Protein Do Not Show a genomes uncoupled (gun) Mutant Phenotype

Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana

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