A functional extracellular matrix biomaterial derived from ovine forestomach

Lun, S.; Irvine, Sharleen M.; Johnson, Keryn D.; Fisher, Neil J.; Floden, Evan; Negron, Leonardo; Dempsey, Sandi G.; McLaughlin, René; Vasudevamurthy, Madhusudan; Ward, Brian R.

doi:10.1016/j.biomaterials.2010.02.025

Cited by 78 publications

(108 citation statements)

References 47 publications

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“…Although not directed specifically at chronic wounds, the proteomic analyses, both in vitro and in vivo studies of cellular interactions with the wide variety of scaffolds being used for tissue regeneration will provide an opportunity to better understand the composition of the ECM and may lead to development of customized tissue-engineered products that are targeted at specific deficits. 47,77,78 …”

Section: Clinical Application Of Collagen-based Biomaterialsmentioning

confidence: 99%

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Topical Collagen-Based Biomaterials for Chronic Wounds: Rationale and Clinical Application

Gould

2016

Advances in Wound Care

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Section: Clinical Application Of Collagen-based Biomaterialsmentioning

confidence: 99%

“…Decellularization may be accomplished by acid, detergent, enzymatic, and mechanical methods, all of which can affect the biochemical and structural characteristics [47][48][49] ( Fig. 4).…”

Section: Decellularized Ecmmentioning

confidence: 99%

Topical Collagen-Based Biomaterials for Chronic Wounds: Rationale and Clinical Application

Gould

2016

Advances in Wound Care

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“…23 With this method, the collagen better retains its fibril length and density. 23 Also retained during processing are the ECM-associated secondary molecules, including laminin, fibronectin, and glycosaminoglycans, 23 which may also help protect from degradation the de novo matrix produced by the wound fibroblast. The ovine-based CECM dressing has been shown to support cell growth and promote cell differentiation, and its collagen structure is completely remodeled during the regenerative process.…”

Section: Ovine-based Collagen Matrix Dressingmentioning

confidence: 99%

“…Ovine-based CECM dressing material tested in vitro has been shown to enhance cellular adhesion and infiltration. 23 Study of ECMs has more clearly defined the importance of cellular signaling and participation in healing by the structural components of an ECM. As a provisional matrix, cellular signaling and differentiation could be affected to enhance wound healing.…”

Section: Structural Components Of Cecm Dressingmentioning

confidence: 99%

Ovine-Based Collagen Matrix Dressing: Next-Generation Collagen Dressing for Wound Care

Bohn¹,

Liden²,

Schultz

et al. 2016

Advances in Wound Care

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Significance: Broad-spectrum metalloproteinase (MMP) reduction along with inherent aspects of an extracellular matrix (ECM) dressing can bring about improved wound healing outcomes and shorter treatment duration. Initial reports of clinical effectiveness of a new ovine-based collagen extracellular matrix (CECM) dressing demonstrate benefits in chronic wound healing. Recent Advances: CECM dressings are processed differently than oxidized regenerated cellulose/collagen dressings. CECM dressings consist primarily of collagens I and III arranged as native fibers that retain the three-dimensional architecture present in tissue ECM. As such, ovine-based ECM dressings represent a new generation of collagen dressings capable of impacting a broad spectrum of MMP excess known to be present in chronic wounds. Critical Issues: While MMPs are essential in normal healing, elevated presence of MMPs has been linked to wound failure. Collagen has been shown to reduce levels of MMPs, acting as a sacrificial substrate for excessive proteases in a chronic wound. Preserving collagen dressings in a more native state enhances bioactivity in terms of the ability to affect the chronic wound environment. Clinical observation and assessment may not be sufficient to identify a wound with elevated protease activity that can break down ECM, affect wound fibroblasts, and impair growth factor response. Future Directions: Collagen dressings that target broad-spectrum excessive MMP levels and can be applied early in the course of care may positively impact healing rates in difficult wounds. Next-generation collagen dressings offer broader MMP reduction capacity while providing a provisional dermal matrix or ECM. SCOPE AND SIGNIFICANCESelecting treatment based on a scientific understanding of the pathological condition of a chronic wound is important in addressing cost and patient needs. Harmful effects of excessive metalloproteinase (MMP) activity in chronic wound healing should be a consideration of wound care decision making. Collagen dressings have been shown to reduce MMP levels, acting as a sacrificial substrate for excessive proteases in chronic wounds. 1-3New ovine-based collagen extracellular matrix (CECM) dressings (EndoformÔ dermal template; Hollister Wound Care, Libertyville, IL) exhibit broad-spectrum MMP inhibition and can serve as a provisional matrix to affect cellular signaling and differentiation that may enhance wound healing. 2 TRANSLATIONAL RELEVANCEDegradation of collagen is important for the physiological remodeling of connective tissues during wound healing. Extracellular degradation of collagen fibers is mediated by MMPs and is a multistep process. A collagen dressing with a preserved structural component can serve as a provisional ECM dermal template and influence cellular interaction necessary to encourage keratinocyte migration. 4 CECM dressings have been shown to retain the complex collagen architecture of native tissue ECM as well as the ECM-associated secondary molecules, including laminin, fibronectin, and glycosam...

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“…The Endoform dermal template consists of a naturally derived ovine collagen ECM. 42 All wounds were covered with a compressible secondary dressing and covered with Vetrap (3 M).…”

Section: Ischemic Wound Modelmentioning

confidence: 99%

Evaluation of a bilayered, micropatterned hydrogel dressing for full-thickness wound healing

Magin

Neale

Drinker

et al. 2016

Exp Biol Med (Maywood)

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Nearly 12 million wounds are treated in emergency departments throughout the United States every year. The limitations of current treatments for complex, full-thickness wounds are the driving force for the development of new wound treatment devices that result in faster healing of both dermal and epidermal tissue. Here, a bilayered, biodegradable hydrogel dressing that uses microarchitecture to guide two key steps in the proliferative phase of wound healing, re-epithelialization, and revascularization, was evaluated in vitro in a cell migration assay and in vivo in a bipedicle ischemic rat wound model. Results indicate that the Sharklet TM -micropatterned apical layer of the dressing increased artificial wound coverage by up to 64%, P ¼ 0.024 in vitro. In vivo evaluation demonstrated that the bilayered dressing construction enhanced overall healing outcomes significantly compared to untreated wounds and that these outcomes were not significantly different from a leading clinically available wound dressing.Collectively, these results demonstrate high potential for this new dressing to effectively accelerate wound healing.Keywords: Micropattern, wound healing, biomaterials, hydrogel, gelatin, microarchitectureExperimental Biology and Medicine 2016; 241: 986-995.

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A functional extracellular matrix biomaterial derived from ovine forestomach

Cited by 78 publications

References 47 publications

Topical Collagen-Based Biomaterials for Chronic Wounds: Rationale and Clinical Application

Topical Collagen-Based Biomaterials for Chronic Wounds: Rationale and Clinical Application

Ovine-Based Collagen Matrix Dressing: Next-Generation Collagen Dressing for Wound Care

Evaluation of a bilayered, micropatterned hydrogel dressing for full-thickness wound healing

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