A fully automated light/dark apparatus useful for comparing anxiolytic agents

Young, Richard; Johnson, D.

doi:10.1016/0091-3057(91)90078-g

Cited by 116 publications

(65 citation statements)

References 9 publications

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Section: Discussionmentioning

confidence: 99%

“…The light dark test has been widely used for modeling anxiety, and it has been developed for predicting the efficacy of clinically used compounds for treating this disease (Young & Johnson, 1991). It has been assumed that the time mice spend in the illuminated side of the box is the most useful and consistent parameter of anxiety (Young & Johnson, 1991). Administration of OS produces a significant response in the light dark test of anxiety, as the time spent by animals in the light chamber increases significantly as compared with the control group, indicating an anxiolytic activity of the extract.…”

Section: Discussionmentioning

confidence: 99%

“…Each animal was placed at the junction of the light dark compartment, facing the illuminated compartment. The time spent in illuminated places was recorded for 5 min (Young & Johnson, 1991). After each test, the box was carefully cleaned up with a wet tissue paper (10% ethanol solution).…”

Section: Light Dark Testmentioning

confidence: 99%

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Evaluation of ethanol leaf extract of Ocimum sanctum in experimental models of anxiety and depression

Chatterjee

Verma

Maurya

et al. 2011

Pharmaceutical Biology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of ethanol leaf extract of Ocimum sanctum in experimental models of anxiety and depression

Chatterjee

Verma

Maurya

et al. 2011

Pharmaceutical Biology

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“…The light-dark and elevated plus maze tests which were used to explore the anxiolytic potentials of H. annuus, where 200mg/kg dose showed moderate anxiolytic properties. Our study showed significant anxiolytic effect in light-dark box test (Young and Johnson, 1991) in compared to elevated plus maze test which showed moderate anxiolytic activity.…”

Section: Discussionmentioning

confidence: 84%

Central nervous system activity of the methanol extracts of Helianthus annuus seeds in mice model

Islam¹,

Islam

Hossain³

et al. 2015

Int Curr Pharm J

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Helianthus annuus seeds contain various chemical components and evaluate different biological activities. The present study was carried out to investigate the central nervous system (CNS) activity of methanolic extract of Helianthus annuus seeds in mice model. General behaviour, antidepressant activity and anxiolytic activity was observed. The results revealed that the methanol extract of Helianthus annuus seeds at 100 and 200 mg/kg caused a significant increase in the spontaneous activity (general behavioural profile), moderate increase in anxiolytic activity (light-dark box and elevated plus maze test) and remarkable increase in antidepressant activity (tail suspension test). The results suggest that methanol extract of Helianthus annuus exhibit significant antidepressant and moderate anxiolytic activity in tested animal models.Islam et al., International Current Pharmaceutical Journal, December 2015, 5(1): 1-4

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“…Η χορήγηση κροκινών σε χαμηλότερες δόσεις (15 και 30 mg/kg) δεν επηρέασε τα συμπεριφορά των πειραματοζώων, γεγονός που υποδηλώνει πως η δράση των κροκινών στην εν λόγω πειραματική δοκιμασία ήταν δοσοεξαρτώμενη. Έχει βρεθεί ότι ο συνολικός χρόνος παραμονής στο φωτεινό διαμέρισμα είναι πιο ευαίσθητος δείκτης αγχολυτικής δράσης από ό,τι ο αριθμός των διελεύσεων μεταξύ των διαμερισμάτων, γιατί ο αριθμός των διελεύσεων αποτελεί και δείκτη της εξερευνητικής δραστηριότητας του ζώου (Kilfoil et al, 1989;Young & Johnson, 1991;Lepicard et al, 2000). Τα ευρήματά μας συνάδουν με τα δεδομένα αυτά.…”

Section: κροκίνες και άγχοςunclassified

Διερεύνηση της δράσης των παραγώγων του Crocus sativus L. επί του κεντρικού νευρικού συστήματος στον επίμυ

Γεωργιάδου¹

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Crocus sativus L. is a rare plant, known since antiquity, which is cultivated in countries ofthe Mediterranean sea and of central Asia. The dried red stigmas of its flowers consist the drugand contain significant amounts of water-soluble carotenoids, known as crocins. Crocins arecrocetin glycosides and are considered responsible for the plant’s extensive therapeuticproperties.Various studies have demonstrated crocins’ chemopreventive, antitumoral, antioxidant,anti-inflammatory and cardioprotective properties. Regarding the central nervous system there isevidence that crocins act beneficially in learning and memory processes, exhibit analgesic effectsand alleviate symptoms in experimental models of epilepsy. Furthermore clinical studies haverevealed their antidepressant effect. Since it remains unknown if this drug affects otherpathophysiological routes of the central nervous system, the aim of the present study was toinvestigate the possible beneficial action of crocins on experimental models of anxiety,obsessive-compulsive disorder and schizophrenia.In order to examine the effect of crocins on anxiety we selected the light/dark test, anexperimental model which evaluates the anxiolytic action of compounds in rodents and is basedon their innate aversion to brightly illuminated areas. Results showed that crocins exhibit aremarkable anxiolytic effect, similar to that of diazepam.For the evaluation of crocins’ effects on obsessive-compulsive disorder we selectedm–chlorophenylpiperazine (m-CPP), a serotoninergic 5–ΗΣ2c agonist, which is known to induceexcessive self-grooming in rats. Excessive self- grooming is considered as a model ofobsessive-compulsive behavior in rodents. The analysis of the results demonstrated that crocinsantagonized the action of mCPP and reduced the time spent by rats in self-grooming.Finally in order to examine crocins’ efficacy in experimental models of schizophrenia weused ketamine, a non-competitive NMDA antagonist, which in subanesthetic dosage produces apsychotomimetic syndrome, characterized by hyperlocomomotion, ataxia, stereotypies, socialwithdrawal and working memory deficits. To determine crocins’ action against the whole spectrumof symptoms we performed three behavioral techniques: an open field test for the motordisorders, which are considered analogs of positive symptoms of schizophrenia, the socialinteraction test for the evaluation of social withdrawal, which serves as a model for the negativesymptoms and the novel object recognition task for the memory deficits, which mimic thecognitive deficits of patients with schizophrenia. Administration of crocins alleviated the motordisorders and antagonized the social withdrawal and memory deficits, caused by ketamine

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A fully automated light/dark apparatus useful for comparing anxiolytic agents

Cited by 116 publications

References 9 publications

Evaluation of ethanol leaf extract of Ocimum sanctum in experimental models of anxiety and depression

Evaluation of ethanol leaf extract of Ocimum sanctum in experimental models of anxiety and depression

Central nervous system activity of the methanol extracts of Helianthus annuus seeds in mice model

Διερεύνηση της δράσης των παραγώγων του Crocus sativus L. επί του κεντρικού νευρικού συστήματος στον επίμυ

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