A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE–HPLC for determination of ochratoxin A and citrinin in lager beers

Lhotská, Ivona; Šatı́nský, Dalibor; Havlíková, Lucie; Solich, Petr

doi:10.1007/s00216-016-9402-6

Cited by 35 publications

(32 citation statements)

References 41 publications

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“…To fully elute the OTA from the adsorbents, the use of appropriate desorption solvent is important. According to the literatures, the desorption solvent should be acidified to around pH 3.3 [13,42]. So we chose pH 3.3 in this experiment.…”

Section: Desorption Solvent and Desorption Timementioning

confidence: 99%

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Wan

Zhang

Bai

et al. 2017

J of Separation Science

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A new, rapid, green, and cost-effective magnetic solid-phase extraction of ochratoxin A from red wine samples was developed using polydopamine-coated magnetic multi-walled carbon nanotubes as the absorbent. The polydopamine-coated magnetic multi-walled carbon nanotubes were fabricated with magnetic multi-walled carbon nanotubes and dopamine by an in situ oxidative self-polymerization approach. Transmission electron microscopy, dynamic light scattering, X-ray photoelectron spectroscopy and vibrating sample magnetometry were used to characterize the absorbents. Ochratoxin A was quantified with high-performance liquid chromatography coupled with fluorescence detection, with excitation and emission wavelengths of 338 and 455 nm, respectively. The conditions affecting the magnetic solid-phase extraction procedure, such as pH, extraction solution, extraction time, absorbent amount, desorption solution and desorption time were investigated to obtain the optimal extraction conditions. Under the optimized conditions, the extraction recovery was 91.8-104.5% for ochratoxin A. A linear calibration curve was obtained in the range of 0.1-2.0 ng/mL. The limit of detection was 0.07 ng/mL, and the limit of quantitation was 0.21 ng/mL. The recoveries of ochratoxin A for spiked red wine sample ranged from 95.65 to 100.65% with relative standard deviation less than 8%. The polydopamine-coated magnetic multi-walled carbon nanotubes showed a high affinity toward ochratoxin A, allowing selective extraction and quantification of ochratoxin A from complex sample matrixes.

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Section: Desorption Solvent and Desorption Timementioning

confidence: 99%

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Wan

Zhang

Bai

et al. 2017

J of Separation Science

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“…Therefore, rapid and convenient detection method for OTA was showed vital importance for food safety [5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Novel fluoroimmunoassays for detecting ochratoxin A using CdTe quantum dots

Yao

Xing

Han

et al. 2016

Journal of Biophotonics

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Novel direct and indirect competitive fluorescence-linked immunosorbent assays (cFLISA and icFLISA) for detection of ochratoxin A (OTA) were described using CdTe quantum dots (QDs) as fluorescent label. CdTe QDs were successfully synthesized, which had an emission wavelength of 615 nm. The high purity monoclonal antibody against OTA was prepared through cell thawing and the octylic acid-ammonium sulfate method. The OTA MAbs were successfully coupled with CdTe QDs, and which also retained the original biological activity. The 50% inhibition values (IC ) of the cFLISA and icFLISA were 0.630 ng/mL, 0.234 ng/mL, the limits of detection (LOD) were 7.06 × 10 and 4.15 × 10 ng/mL, and detection ranges were 7.06 × 10 - 18.34 ng/mL and 4.15 × 10 - 4.88 ng/mL, in-order. The recoveries were 96.0-104.7% along with coefficients of variation (CVs) below 10%. The FLISA provided novel method for determination of OTA and the potential of MAb-CdTe QDs for the establishment of fluorescent immunochromatographic test strip.

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“…The most commonly used technique to detect OTA is HPLC with fluorescence detection, because OTA has natural fluorescence [18][19][20][21][22]. Sample clean-up is a key step for detecting trace amounts of OTA in complex matrixes.…”

Section: Introductionmentioning

confidence: 99%

High‐performance liquid chromatography with fluorescence detection of ochratoxin A in cereal, coffee, and wine: Effective pretreatment with bovine serum albumin‐immobilized adsorbent

Yamamoto

Sawada

Yamato

et al. 2018

Separation Science Plus

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Ochratoxin A is a fluorescent mycotoxin that is present in many foods. It is difficult to detect by high‐performance liquid chromatography because of interfering substances. We immobilized bovine serum albumin on resin and used it to extract ochratoxin A from various food samples. Sample extracts were applied to a cartridge packed with bovine serum albumin‐immobilized resin. The adsorbed ochratoxin A was eluted by octanoate, which is a serum albumin ligand. The chromatograms of the eluate showed no endogenous peaks that interfered with ochratoxin A detection. The recovery rates of ochratoxin A from coffee and wine samples were more than 82% with relative standard deviations of less than 5%. The positive detection result in the certified reference material of wheat agreed with its certification value. This clean‐up method with bovine serum albumin‐immobilized resin has great potential for assessing compliance of foods with ochratoxin A regulations.

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A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE–HPLC for determination of ochratoxin A and citrinin in lager beers

Cited by 35 publications

References 41 publications

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Novel fluoroimmunoassays for detecting ochratoxin A using CdTe quantum dots

High‐performance liquid chromatography with fluorescence detection of ochratoxin A in cereal, coffee, and wine: Effective pretreatment with bovine serum albumin‐immobilized adsorbent

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