A fully automated analyzer for multiple detection of allergen-specific immunoglobulin E

Abstract

Allergy testing is important for evidence-based care and treatment. In this paper, we report a new clinical laboratory instrument, AdvanSure AlloStation Smart, which is used for a multiple allergen-specific immunoglobulin (IgE) assay. For efficient automation of immunoblot procedures, a novel tilting carousel technology was incorporated. The analyzer showed 100.5-101.7% of pipetting accuracy, no sample carryover, and 0.2-3.2% coefficient of variation of optical precision. Assay performance on the system was comparable with that of a single allergen test, ImmunoCAP for 23 allergens. The slope and the intercept of this comparison were 0.994 and 0.070, respectively. The Pearson correlation coefficient between two methods was r ¼ 0.836. The coefficient of variation of intra-assay precision ranged from 3.2% to 11.1% and that of inter-assay precision varied from 4.0% to 17.6%. The AdvanSure AlloStation Smart provides a simple and reliable multiple assay platform to monitor allergen-specific IgE profiles.

A theoretical model of healthcare monitoring surveillance system for patients with severe allergies

“…It tests the allergic reaction when the patient takes oral food. It has identified allergic subjects 17 min earlier than the trained clinicians (Oh et al, 2015;Twomey et al, 2013). Regarding the monitoring of environmental allergens and airborne allergens (Wu et al, 2014;Bastl et al, 2016), atmospheric pressure cold plasma (APCP) treatment which includes further tests using blood sera from the allergen sensitized humans reduces the size of environmental allergens and airborne allergens and prevents the allergic diseases.…”