A Full Expression of the Genome of Rous Sarcoma Virus in Heterokaryons Formed after Fusion of Virogenic Mammalian Cells and Chicken Fibroblasts

Machala, O; Donner, Ludvik R.; Svoboda, Jan

doi:10.1099/0022-1317-8-3-219

Cited by 30 publications

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“…obtained with the virogenic line of Chinese hamster cells transformed by SR-RSV (Machala et al, 1970) and suggests that infectious RSV is, in general, produced after fusion of virogenic mammalian cells with indicator cells.…”

Section: Testing Of the Capacity Of Differentiated Chicken Cells To Amentioning

confidence: 97%

Comparative study of RSV rescue from RSV‐transformed mammalian cells

Svoboda

Machala

Donner

et al. 1971

Intl Journal of Cancer

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All individual heterokaryons obtained by fusion of cells of the five different mammalian RSV-transformed virogenic cells with indicator chicken fibroblasts produced infectious RSV. In contrast, non-virogenic mammalian cells derived from tumours which arose in mice injected with RSV did not give rise to infectious RSV, even when fused with chicken fibroblasts preinfected with three different "helper viruses". Fusion of four lines of vlrogenic cells with mammalian fibroblasts as well as of virogenic Chinese hamster cells with chicken liver, mesonephros, thymus and macrophage cells did not lead to the formation of detectable RSVor RSV coat antigen. Similarly, experiments on complementation by mutual fusion of virogenic cells in five different crosses were negative. The presence of a " natural" Gs antigen in the indicator cells is not necessary for RS V rescue because the fusion ofcells derived from Gs negative chicken and duck embryos with virogenic cells leads to the formation of infectious RSV.The results are discussed in relation to the specificity of the cells participating in the induction of RS V formation by cell fusion.

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Section: Testing Of the Capacity Of Differentiated Chicken Cells To Amentioning

confidence: 97%

Comparative study of RSV rescue from RSV‐transformed mammalian cells

Svoboda

Machala

Donner

et al. 1971

Intl Journal of Cancer

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“…During a stay in London at Imperial Cancer Research Fund, I performed quantitative experiments showing a 100-fold increase in the efficiency of RSV rescue and obtained a good correlation between the degree of virus rescue and the number of heterokaryons (18). Finally, the study of individual heterokaryons provided evidence that they produced infectious RSV (19).…”

Section: Rsv Rescue From Virogenic Cellsmentioning

confidence: 99%

On board a raft or boat in the retrovirus sea

Svoboda¹

2016

Proc. Natl. Acad. Sci. U.S.A.

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This article summarizes the essential steps in understanding the chicken Rous sarcoma virus (RSV) genome association with a nonpermissive rodent host cell genome. This insight was made possible by in-depth study of RSV-transformed rat XC cells, which were called virogenic because they indefinitely carry virus genetic information in the absence of any infectious virus production. However, the virus was rescued by association of XC cells with chicken fibroblasts, allowing cell fusion between both partners. This and additional studies led to the interpretation that the RSV genome gets integrated into the host cell genome as a provirus. Study of additional rodent virogenic cell lines provided evidence that the transcript of oncogene v-src can be transmitted to other retroviruses and produce cell transformation by itself. As discussed in the text, two main questions related to nonpermissiveness to retrovirus infection remain to be solved. The first is changes in the retrovirus envelope gene allowing virus entry into a nonpermissive cell. The second is the nature of the permissive cell functions required by the nonpermissive cell to ensure infectious virus production. Both lines of investigation are being pursued.

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“…However, the virus can be rescued if conditions are created that make possible heterokaryon formation between trans formed mammalian cells and CEF [Svoboda, 1962;SimkoviC et al, 1962;Svoboda et al, 1967;Vigier, 1967;Machala et al, 1970], All of these findings, of course, hold only for mammalian cells transformed by nondefec tive RSV strains, such as those used through out the above studies. Nevertheless, even af ter using an initially nondefective RSV, transformed mammalian cell lines that can be rescued only by fusion with CEF prein fected with a helper virus can often be ob tained [Popovic et al, 1977;Steimer and Boettiger, 1977], In this case, the helper virus complements some defect in the provirus that is integrated in the mammalian cell.…”

Section: Virus-cell Interactionmentioning

confidence: 99%

Rous Sarcoma Virus

Svoboda¹

1986

Intervirology

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A Full Expression of the Genome of Rous Sarcoma Virus in Heterokaryons Formed after Fusion of Virogenic Mammalian Cells and Chicken Fibroblasts

Cited by 30 publications

References 0 publications

Comparative study of RSV rescue from RSV‐transformed mammalian cells

Comparative study of RSV rescue from RSV‐transformed mammalian cells

On board a raft or boat in the retrovirus sea

Rous Sarcoma Virus

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