A freeze-dried formulation of bacteriophage encapsulated in biodegradable microspheres

Puapermpoonsiri, Utsana; Spencer, Janice; Walle, Christopher F. van der

doi:10.1016/j.ejpb.2008.12.001

Cited by 98 publications

(75 citation statements)

References 38 publications

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“…This study found that freeze-drying was an efficient method to dry phages with little decrease in phage activity, which would help in developing different paper-coating approaches for the commercial manufacture of phage-containing bioactive membranes. The same technique has been used for the encapsulation of Staphylococcus aureus and Pseudomonas aeruginosa phages without affecting their lytic activity (29,30).…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Anany

Chen

Pelton

et al. 2011

Appl Environ Microbiol

199

140

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The ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. The range of applications of bacteriophage can be extended by their immobilization on inert surfaces. A novel method for the oriented immobilization of bacteriophage has been developed. The method was based on charge differences between the bacteriophage head, which exhibits an overall net negative charge, and the tail fibers, which possess an overall net positive charge. Hence, the head would be more likely to attach to positively charged surfaces, leaving the tails free to capture and lyse bacteria. Cellulose membranes modified so that they had a positive surface charge were used as the support for phage immobilization. It was established that the number of infective phages immobilized on the positively charged cellulose membranes was significantly higher than that on unmodified membranes. Cocktails of phages active against Listeria or Escherichia coli immobilized on these membranes were shown to effectively control the growth of L. monocytogenes and E. coli O157:H7 in ready-to-eat and raw meat, respectively, under different storage temperatures and packaging conditions. The phage storage stability was investigated to further extend their industrial applications. It was shown that lyophilization can be used as a phage-drying method to maintain their infectivity on the newly developed bioactive materials. In conclusion, utilizing the charge difference between phage heads and tails provided a simple technique for oriented immobilization applicable to a wide range of phages and allowed the retention of infectivity.

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Section: Discussionmentioning

confidence: 99%

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Anany

Chen

Pelton

et al. 2011

Appl Environ Microbiol

199

140

View full text Add to dashboard Cite

show abstract

“…Однако исследования микрокристаллов и микросфер не показывают значительных результатов, так как при хранении происходит потеря титра относительно быстро (не более 6 недель при 4⁰C) [41][42][43].…”

Section: микрокристаллы и микросферыunclassified

Design of Therapeutic Phage Cocktails Based on T4-Type Bacteriophages: Advantages and Disadvantages

Nikulin¹,

Коnоnеnко²,

Koshchayev³

et al. 2017

Polythematic Online Scientific Journal of Kuban State Agrarian University

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“…Puapermpoonsiri et al [56] showed that the stability of the bacteriophage in the nasal inserts was far greater than for encapsulated phage in polyestermatrices, wherein no lytic activity was observed after 1 week and Wright et al [57] showed that the bacteriophage titer in the inserts after 12 months may still represent a therapeutic dose. The first controlled clinical trial of a therapeutic bacteriophage preparation showed efficacy and safety in chronic otitis because of chemo-resistant P. Aeruginosa [56]. Following bacteriophage therapy of methicillin-resistant Staphylococcus aureus with a nasal insert was studied by Alfadhel et al [58].…”

Section: Overview Of Work On Nasal Insertsmentioning

confidence: 99%

“…Bacteriophage include nasal delivery was studied by some researhers [56][57][58]. Puapermpoonsiri et al [56] showed that the stability of the bacteriophage in the nasal inserts was far greater than for encapsulated phage in polyestermatrices, wherein no lytic activity was observed after 1 week and Wright et al [57] showed that the bacteriophage titer in the inserts after 12 months may still represent a therapeutic dose.…”

Section: Overview Of Work On Nasal Insertsmentioning

confidence: 99%

“…In the context of the foregoing, nasal delivery studies are few despite its many advantages, probably owing to some limitations. However, studies on nasal inserts of some active molecules and proteins using mucoadhesive polymers such as cellulose derivatives, acrylic acid derivatives, polysaccarides and gums are outlined here [38,[46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62].…”

Section: Overview Of Work On Nasal Insertsmentioning

confidence: 99%

See 1 more Smart Citation

Nasal Inserts for Drug Delivery: An Overview

Algin-Yapar¹

2014

Trop. J. Pharm Res

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A freeze-dried formulation of bacteriophage encapsulated in biodegradable microspheres

Cited by 98 publications

References 38 publications

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Design of Therapeutic Phage Cocktails Based on T4-Type Bacteriophages: Advantages and Disadvantages

Nasal Inserts for Drug Delivery: An Overview

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