A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications

Tang, Qian; Lim, Thou; Wei, Xiaojuan; Wang, Qiyang; Jian-chen, XU; Shen, Liyan; Zhu, Zhenzhong; Zhang, Changqing

doi:10.1016/j.biomaterials.2020.120138

Cited by 42 publications

(30 citation statements)

References 63 publications

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“…Immunohistochemistry and Masson staining showed that, in the process of wound healing, UA promoted collagen deposition and remodeling, which was characterized by a large number of collagen fibers arranged under the wound ( Leivonen et al, 2005 ; Werner et al, 2007 ; Brem et al, 2009 ). During the healing process, the proper deposition of these two types of collagen fibers (collagen I and III) will facilitate wound healing and obtain a better healing area ( Tang et al, 2020 ). In addition, the expression of cytokeratin, that is, the degree of keratinization, could increase its expression after UA treatment, indicating that UA could promote re-epithelialization of wound regeneration.…”

Section: Discussionmentioning

confidence: 99%

Urolithin A Promotes Angiogenesis and Tissue Regeneration in a Full-Thickness Cutaneous Wound Model

et al. 2022

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The treatment of chronic wound is an important topic of current clinical issue. Neovascularization plays a crucial role in skin wound healing by delivering fresh nutrients and oxygen to the wound area. The aim of this study was to investigate the mechanisms of urolithin A (UA) in angiogenesis during wound healing. The results of in vitro experiments showed that treatment with UA (5–20 μM) promoted the proliferation, migration, and angiogenic capacity of HUVECs. Furthermore, we investigated the effect of UA in vivo using a full-thickness skin wound model. Subsequently, we found that UA promoted the regeneration of new blood vessels, which is consistent with the results of accelerated angiogenesis in vitro experiments. After UA treatment, the blood vessels in the wound are rapidly formed, and the deposition and remodeling process of the collagen matrix is also accelerated, which ultimately promotes the effective wound healing. Mechanistic studies have shown that UA promotes angiogenesis by inhibiting the PI3K/AKT pathway. Our study provides evidence that UA can promote angiogenesis and skin regeneration in chronic wounds, especially ischemic wounds.

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Section: Discussionmentioning

confidence: 99%

Urolithin A Promotes Angiogenesis and Tissue Regeneration in a Full-Thickness Cutaneous Wound Model

et al. 2022

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“…In vitro studies on primary human umbilical vein endothelial cells and fibroblasts showed that they were effective substrates for angiogenesis and fibrogenesis. The release of the proteins was sustained for many days while crosslinked films showed slower kinetics [100].…”

Section: Polyelectrolyte-based Films For Sustained Drug Releasementioning

confidence: 99%

“…These materials had adequate thermal properties and a glass transition temperature that could be increased by the content of sodium alginate. Highly hydrophilic hyaluronic acid/poly-l-lysine multilayer constructions could create free standing films for the delivery of platelet lycates in healing of cutaneous injuries [100]. In vitro studies on primary human umbilical vein endothelial cells and fibroblasts showed that they were effective substrates for angiogenesis and fibrogenesis.…”

Section: Polyelectrolyte-based Films For Sustained Drug Releasementioning

confidence: 99%

Current Research on Polyelectrolyte Nanostructures: From Molecular Interactions to Biomedical Applications

Papagiannopoulos¹

2021

Macromol

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Polyelectrolytes have been at the center of interdisciplinary research for many decades. In the field of polymer science and soft matter, they have provided the dimensions of electrostatic interactions, which opens a vast variety of opportunities for new physical properties and applications. In biological matter, polyelectrolytes are present in many forms, from extracellular polysaccharides to complex DNA molecules and proteins. This review discusses the recent research on polyelectrolytes covering the fundamental level of their conformations and nanostructures, their molecular interactions with materials that have close relevance to bioapplications and their applications in the biomedical field. This approach is motivated by the fact that the polyelectrolyte research is constantly active in all the aforementioned levels and continually affects many critical scientific areas.

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“… 28 , 29 Previous reports showed that PL exerted regenerative effects on various tissues and cells, such as skin, cartilage, tendon cells and nerve cells. 30 , 31 , 32 Recently, our studies demonstrated that PL exerted protective effects on chondrocytes and cartilage against osteoarthritis through regulation of NF‐κB signalling pathway, and that PL benefited umbilical cord‐derived MSCs (huc‐MSCs) by promoting cell proliferation, cell cycle progression and cell migration through activation of AMPK/mTOR signalling pathway‐mediated autophagy. 28 Moreover, we found that the growth factors (PDGF, IGF‐1, TGF‐β and EGF) contributed to the regenerative effects of PL in varying degrees.…”

Section: Introductionmentioning

confidence: 99%

Growth factors‐based platelet lysate rejuvenates skin against ageing through NF‐κB signalling pathway: In vitro and in vivo mechanistic and clinical studies

Lu²,

Zhou

et al. 2022

Cell Proliferation

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Introduction Platelets benefit tissue regeneration by secreting growth factors, and platelet products, for example, platelet lysate (PL), have been clinically applied for tissue rejuvenation. To determine the anti‐ageing efficacy and mechanism of human PL (hPL) on skin, this study conducted clinical retrospective analysis, nude mice‐based in vivo study and human dermal fibroblasts (HDFs)‐based in vitro study. Methods Flow cytometry was employed for quality control of hPL, and ELISA was used for quantification of growth factors (EGF, IGF‐1, PDGF and TGF‐β) in hPL. After d ‐galactose modelling, skin texture grading, histopathological observation, immunofluorescence analysis and oxidative stress assays were conducted on nude mice, while SA‐β‐gal staining, CCK‐8 and wound healing assays were conducted on HDFs. qPCR and western blot were conducted to clarify hPL's mechanism. Results The clinical retrospective data showed that hPL obviously rejuvenated human skin appearances without adverse events. The animal data showed that hPL exerted rejuvenative effects on skin, and the cellular data showed that hPL significantly promoted the proliferation and migration of HDFs and suppressed senescence‐associated secretory protein secretion and senescence state of senescent HDFs by suppressing NF‐κB pathway. The NF‐κB‐dependent mechanism was verified positively by using P65 siRNA and negatively by using prostratin. Furthermore, EGF, IGF‐1, PDGF and TGF‐β were found as the main ingredients in hPL, which contributed to the efficacy and mechanism of hPL. Conclusion This study provided novel knowledge of hPL, making it ideal for skin rejuvenation.

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A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications

Cited by 42 publications

References 63 publications

Urolithin A Promotes Angiogenesis and Tissue Regeneration in a Full-Thickness Cutaneous Wound Model

Urolithin A Promotes Angiogenesis and Tissue Regeneration in a Full-Thickness Cutaneous Wound Model

Current Research on Polyelectrolyte Nanostructures: From Molecular Interactions to Biomedical Applications

Growth factors‐based platelet lysate rejuvenates skin against ageing through NF‐κB signalling pathway: In vitro and in vivo mechanistic and clinical studies

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