A Focused Multiple Reaction Monitoring (MRM) Quantitative Method for Bioactive Grapevine Stilbenes by Ultra-High-Performance Liquid Chromatography Coupled to Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ)

Abstract

Grapevine stilbenes are a family of polyphenols which derive from trans-resveratrol having antifungal and antimicrobial properties, thus being considered as phytoalexins. In addition to their diverse bioactive properties in animal models, they highlight a strong potential in human health maintenance and promotion. Due to this relevance, highly-specific qualitative and quantitative methods of analysis are necessary to accurately analyze stilbenes in different matrices derived from grapevine. Here, we developed a rapid, sensitive, and specific analysis method using ultra-high-performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ) in MRM mode to detect and quantify five grapevine stilbenes, trans-resveratrol, trans-piceid, trans-piceatannol, trans-pterostilbene, and trans-ε-viniferin, whose interest in relation to human health is continuously growing. The method was optimized to minimize in-source fragmentation of piceid and to avoid co-elution of cis-piceid and trans-resveratrol, as both are detected with resveratrol transitions. The applicability of the developed method of stilbene analysis was tested successfully in different complex matrices including cellular extracts of Vitis vinifera cell cultures, reaction media of biotransformation assays, and red wine.

Neuroprotective Mechanisms of Resveratrol in Alzheimer’s Disease: Role of SIRT1

“…The final steps involve the stepwise reactions that promote the decarboxylation, dehydration, and enolization of stilbene-2-carboxylic acid to yield the resveratrol molecule [ 52 ]. Resveratrol can undergo other biochemical reactions to produce new stilbenes, including ε -viniferin, t-piceid, t-piceatannol, and t-pterostilbene [ 53 ].…”

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

“…LC–MS/MS is a rapidly developing technique and has raised bioanalysis to a new level. Multiple reaction monitoring (MRM) based on LC–MS/MS (LC–MRM) is a “gold standard” for the bioanalysis of small molecules [168–181]. MRM assay is a target MS technique based on ESI–MS [182–191].…”

Characterization of Several West Georgian Autochthonous Grapes and Their Wine Stilbenes

“…To prepare a sample, an extraction is carried out using different solvents and concentrates. The best option for extracting from grapes is alcohol mixed with water, and in the case of wine, it is a concentrated using Amberlite resin (Hurtado-Gaitán et al, 2017;López-Hernández et al, 2016;Decendit, 2017). The interest in studying stilbenes is great due to its antioxidant properties.…”